The invention relates to a digital holography method for detecting the vibration amplitude of an object (15) having a vibration frequency ω, comprising: generating object illumination waves (W.sub.t) and reference waves (W.sub.LO); acquiring interferograms between the reference wave (W.sub.LO) and a signal wave (W.sub.s) by means of a bandwidth ω s detector (19), the reference wave comprising two components E.sub.LO1, E.sub.LO1 of frequencies ω.sub.1, ω.sub.2 that are respectively staggered in relation to the laser frequency ω.sub.L by a quantity δ.sub.1=γ.sub.1ω.sub.s and δ2=qω+γ2ω.sub.s, where q is an integer and −0.5≦γ1, γ.sub.2≦0.5; and calculating the vibration amplitude of the object from the optical beats spectrum deduced from the complex amplitude of an interferogram.

A portable inspection probe for the inspection of a recessed mating surface of an optical fiber connector is provided. In one variant, the portable inspection probe includes a digital holographic detection module operable to digitally record a hologram of the recessed mating surface, and a rigid probe tip configured to be optically coupled to the digital holographic detection module and shaped to provide optical access to the recessed mating surface. In another variant, the portable inspection probe is to be used with a rigid probe tip connectable thereto, and the digital holographic detection module includes a probing optical assembly not traversed by a reference beam and configured to direct an object beam onto the recessed mating surface and to collect the object beam upon reflection thereof by the recessed mating surface. An inspection system and an inspection method are also provided.

A super-resolution holographic microscope includes a light source configured to emit input light, a diffraction grating configured to split the input light into first diffracted light and second diffracted light, a mirror configured to reflect the first diffracted light, a wafer stage arranged on an optical path of the second diffracted light and on which a wafer is configured to be arranged, and a camera configured to receive the first diffracted light that is reflected by the mirror and the second diffracted light that is reflected by the wafer to generate a plurality of hologram images of the wafer.

A method for lens-free imaging of a sample or objects within the sample uses multi-height iterative phase retrieval and rotational field transformations to perform wide FOV imaging of pathology samples with clinically comparable image quality to a benchtop lens-based microscope. The solution of the transport-of-intensity (TIE) equation is used as an initial guess in the phase recovery process to speed the image recovery process. The holographically reconstructed image can be digitally focused at any depth within the object FOV (after image capture) without the need for any focus adjustment, and is also digitally corrected for artifacts arising from uncontrolled tilting and height variations between the sample and sensor planes. In an alternative embodiment, a synthetic aperture approach is used with multi-angle iterative phase retrieval to perform wide FOV imaging of pathology samples and increase the effective numerical aperture of the image.

A method of extracting particles from a two-dimensional (2D) hologram recorded as part of a digital inline holography system includes reconstructing a three-dimensional (3D) optical field from the recorded 2D hologram. In addition, particles are extracted/segmented from the 3D optical field, wherein segmented particles are identified by particle location in three-dimensional space and a cross-sectional area of the segmented particle. Based on the identified particle location and cross-sectional area, extracted particles are removed from the 2D hologram to generate an updated 2D hologram. These steps are repeated iteratively until a threshold is met.

Embodiments are directed to an apparatus for creating a scene comprising: a plurality of micro-mirrors configured to rotate between an off position and at least two on positions to generate a plurality of holograms, and a processor configured to select positions for the micro-mirrors based on an input specification of the scene.

The present disclosure relates to systems and methods for cell recognition. At least one embodiment relates to a method for recognizing cell. The method includes receiving an image of the cell. The method also includes performing edge detection on the image of the cell. Further, the method includes detecting ridges within the image of the cell. In addition, the method includes quantifying an internal complexity of the cell by gauging a contrast of the ridges with an average of a Laplacian on the detected ridges.

At least one embodiment relates to an autofocus method for determining a focal plane for at least one object. The method includes reconstructing a holographic image of the at least one object such as to provide a reconstructed image at a plurality of different focal depths. The reconstructed image includes a real component and an imaginary component. The method also include performing a first edge detection on the real component for at least two depths of the plurality of different focal depths and a second edge detection on the imaginary component for the at least two depths. Further, the method includes obtaining a first measure of clarity for each of the at least two depths based on a first measure of statistical dispersion with respect to the first edge detection and a second measure of clarity.

In-line holography to create images of a specimen, such as one or more particles dispersed in a transparent medium. Analyzing these images with results from light scattering theory yields the particles' sizes with nanometer resolution, their refractive indexes to within one part in a thousand, and their three dimensional positions with nanometer resolution. This procedure can rapidly and directly characterize mechanical, optical and chemical properties of the specimen and its medium.

A method and system of imaging a moving object within a microfluidic environment includes illuminating a first side of a flow cell configured to carry the moving object within a flow of carrier fluid with an illumination source emitting at least partially coherent light, the at least partially coherent light passing through an aperture prior to illuminating the flow cell. A plurality of lower resolution frame images of the moving object are acquired with an image sensor disposed on an opposing side of the flow cell, wherein the image sensor is angled relative to a direction of flow of the moving object within the carrier fluid. A higher resolution image is reconstructed of the moving object based at least in part on the plurality of lower resolution frame images.