Techniques to improve image quality in holography utilizing lenses made from materials with non-quantized anisotropic electromagnetic properties, such as birefringent materials, to advantageously split an incoming beam of light into two coincident beams with different focal lengths that interfere with one another and thus create holograms free of electro-optical or pixelated devices are disclosed. Corresponding systems, methods and apparatuses are described.

A method for performing color image reconstruction of a single super-resolved holographic sample image includes obtaining a plurality of sub-pixel shifted lower resolution hologram images of the sample using an image sensor by simultaneous illumination at multiple color channels. Super-resolved hologram intensity images for each color channel are digitally generated based on the lower resolution hologram images. The super-resolved hologram intensity images for each color channel are back propagated to an object plane with image processing software to generate a real and imaginary input images of the sample for each color channel. A trained deep neural network is provided and is executed by image processing software using one or more processors of a computing device and configured to receive the real input image and the imaginary input image of the sample for each color channel and generate a color output image of the sample.

According to an embodiment, a holographic microscope comprises a light source, an optical system splitting light emitted from the light source into an object and a reflective mirror and inducing interference between light reflected by the object or transmitted through the object and light reflected by the reflective mirror, a first image sensor receiving the interference light and sensing interference information for the interference light, a second image sensor receiving the light reflected by the object or transmitted through the object and sensing information for the received light, and an image processor deriving a shape of the object based on the interference information sensed by the first image sensor and the information sensed by the second image sensor.

According to an aspect of the present inventive concept there is provided a device for imaging of a microscopic object, the device comprising: an array of light sensitive areas, each being sensitive to detect light spanning a wavelength range of at least 400-1200 nm; at least one light source configured to generate light at a plurality of wavelengths within the wavelength range, comprising at least one wavelength in a visible part of the wavelength range and at least one wavelength in a short-wave infrared, SWIR, part of the wavelength range, and arranged to illuminate the microscopic object with the generated light such that at least part of the light is scattered by the microscopic object; wherein the device is configured to transmit the scattered light and non-scattered light, from the same light source, to the array of light sensitive areas configured to detect an interference pattern formed between the scattered light and the non-scattered light, for each wavelength.

According to an aspect of the present inventive concept there is provided a device for imaging of a microscopic object, the device comprising: an array of light sensitive areas sensitive to detect light spanning a wavelength range of at least 400-1200 nm; at least one light source comprising at least a first point of operation in which the at least one light source is configured to generate visible light, and a second point of operation in which the at least one light source is configured to generate infrared light, and being arranged to illuminate the microscopic object such that light is scattered by the microscopic object; wherein the array of light sensitive areas is configured to detect an interference pattern formed between the scattered light and non-scattered light; the device being configured to be set in a selected point of operation from the at least first and second points of operation, for detecting the interference pattern for imaging the microscopic object at a wavelength defined by the selected point of operation.

A polarization holographic microscope system is disclosed. The polarization holographic microscope system can acquire a birefringence image and a three-dimensional phase image with high sensitivity by aperture synthesis of sample beams at various angles, and a sample image acquisition method using the microscope system.

A totagram is produced by an iterative spectral phase recovery process resulting in complete information recovery using special masks, without a reference beam. Using these special masking systems reduce computation time, number of masks, and number of iterations. The special masking system is (1) a unity mask together with one or more bipolar binary masks with elements equal to 1 and −1, or (2) a unity mask together with one or more phase masks, or (3) a unity mask together with one pair of masks or more than one pair of masks having binary amplitudes of 0's and 1's, in which the masks in the pair are complementary to each other with respect to amplitude, or (4) one or more pairs of complementary masks with binary amplitudes of 0's and 1's without a unity mask.

A microscope comprising a coherent light source producing a coherent light beam, a light beam guide system comprising a beam splitter configured to split the coherent light beam into a reference beam and a sample illumination beam, a sample holder configured to hold a sample to be observed, a sample illumination device configured to direct the sample illumination beam through the sample and into a microscope objective, a beam reuniter configured to reunite the reference beam and sample illumination beam after passage of the sample illumination beam through the sample to be observed, and a light sensing system configured to capture at least phase and intensity values of the coherent light beam downstream of the beam reuniter.

An apparatus and method are introduced to produce a hologram of an object from electromagnetic radiation, such as incoherent light, received from the object. The electromagnetic radiation is received by a receiving assembly and transformed into a plurality of co-linear co-propagating beams with different focal distances. The interference of the plurality of beams is enabled by projecting components of each beam along a common polarization direction. The interference patterns thus formed are recorded and then processed to form the hologram of the object.

A digital hologram is represented by a set of coefficients respectively associated with a plurality of definition wavelets each defined by a tuple of coordinates in a multidimensional space. A method for reconstructing the digital hologram in order to display it by a display, includes the following steps: depending on at least one data item representative of a characteristic of the display, determining a transformation of the multidimensional space; and generating a reconstructed hologram by assigning each coefficient of at least some of the coefficients to a reconstruction wavelet defined by an image reconstruction tuple by the predetermined transformation of the tuple of coordinates defining the definition wavelet associate with the coefficient in question. An associated display method, reconstruction device and system are also described.