A hologram profile optimization method includes: setting a first hologram profile as a variable; and performing an optimization cycle a predetermined number of times, wherein the optimization cycle includes encoding the first hologram profile into a binary hologram profile by using an ApproxSign function; calculating a field value of a holographic image on a display surface for the binary hologram profile, considering high-order diffraction term noise of the holographic image by using a tiling function; calculating an intensity of the holographic image on the display surface; calculating a loss function value based on a difference between the intensity of the holographic image and an intensity of a target image; and updating the first hologram profile to a second hologram profile based on the loss function value.

A method for lens-free imaging of a sample or objects within the sample uses multi-height iterative phase retrieval and rotational field transformations to perform wide FOV imaging of pathology samples with clinically comparable image quality to a benchtop lens-based microscope. The solution of the transport-of-intensity (TIE) equation is used as an initial guess in the phase recovery process to speed the image recovery process. The holographically reconstructed image can be digitally focused at any depth within the object FOV (after image capture) without the need for any focus adjustment, and is also digitally corrected for artifacts arising from uncontrolled tilting and height variations between the sample and sensor planes. In an alternative embodiment, a synthetic aperture approach is used with multi-angle iterative phase retrieval to perform wide FOV imaging of pathology samples and increase the effective numerical aperture of the image.

A method for three-dimensional imaging of a sample (302) comprises: receiving (102) interference patterns (208) acquired using light-detecting elements (212), wherein each interference pattern (208) is formed by scattered light from the sample (302) and non-scattered light from a light source (206; 306), wherein the interference patterns (208) are acquired using different angles between the sample (302) and the light source (206; 306); performing digital holographic reconstruction applying an iterative algorithm to change a three-dimensional scattering potential of the sample (302) to improve a difference between the received interference patterns (208) and predicted interference patterns based on the three-dimensional scattering potential; wherein the iterative algorithm reduces a sum of a data fidelity term and a non-differentiable regularization term and wherein the iterative algorithm includes a forward-backward splitting method alternating between forward gradient descent (108) on the data fidelity term and backward gradient descent (110) on the regularization term.

Apparatuses and methods for comparative holographic imaging to improve structural and molecular information of reconstructions is disclosed herein. An example method at least includes acquiring a plurality of holograms of a sample, wherein each hologram of the plurality of holograms is acquired at a different electron beam energy, and determining atomic and structural information of the sample based at least on a comparison of at least two of the holograms of the plurality of holograms.

An ultrasound emitter launches an ultrasonic signal into a diffuse medium such as tissue. The diffuse medium is illuminated with an infrared illumination signal. activating an ultrasound emitter to launch an ultrasonic signal into a diffuse medium. An infrared reference beam is interfered with an infrared exit signal having an infrared wavelength that is the same as the infrared illumination signal. An infrared image is captured of the interference of the infrared reference beam with the infrared exit signal.

A device and method for forming an image of a sample includes illuminating the sample with a light source; acquiring a plurality of images of the sample using an image sensor, the sample being placed between the light source and the image sensor, no magnifying optics being placed between the sample and the image sensor, the image sensor lying in a detection plane, the image sensor being moved with respect to the sample between two respective acquisitions, such that each acquired image is respectively associated with a position of the image sensor in the detection plane, each position being different from the next; and forming an image, called the high-resolution image, from the images thus acquired.

A holographic imaging device is disclosed. In one aspect, the holographic imaging device comprises an imaging unit comprising at least two light sources, wherein the imaging unit is configured to illuminate an object by emitting at least two light beams with the at least two light sources. A first and second light beams have different wave-vectors and wavelengths. The holographic imaging device further comprises a processing unit configured to obtain at least two holograms of the object by controlling the imaging unit to sequentially illuminate the object with respectively the first light beam and the second light beam, construct at least two 2D image slices based on the at least two holograms, wherein each 2D image slice is constructed at a determined depth within the object volume, and generate a three-dimensional image of the object based on a combination of the 2D image slices.

A system for generating multi-depth image sequence comprising a modulation array. The modulation array comprising a plurality of light modulators which may shift light incident upon the modulators by a number of degrees. The plurality of light modulators may shift light in concert according to a modulation shift pattern. The modulation shift pattern can be configured to focus incident light to a voxel or to form a 3-D image. One or more modulation shift patterns can be changed or cycled through to raster one or more image objects in one or more image depth planes.

An optical device (100) for forming a distribution of a three-dimensional light field comprises: an array (102) of unit cells (104), a unit cell (104) being individually addressable for switching the optical property of the unit cell (104) between a first and a second condition; wherein the unit cells (104) are configured to be selectively active or inactive and wherein the array (102) comprises at least a first and a second disjoint subset (110; 112; 114; 116), and wherein the unit cells (104) in a subset (110; 112; 114; 116) are configured to be jointly switched from inactive to active, wherein the active unit cells (104) are configured to interact with an incident light beam (106) for forming the distribution of the three-dimensional light field; and wherein the optical device (100) is configured to address inactive unit cells (104) for switching the optical property of unit cells (104).

A light detection and ranging system arranged to scan a scene is provided. A light source outputs light having a first characteristic. A spatial light modulator receives output light from the light source and outputs spatially-modulated light in accordance with computer-generated holograms represented thereon. A light detector receives light having the first characteristic from the scene and outputs a light response signal. A holographic controller is arranged to output a plurality of computer-generated holograms to the spatial light modulator. Each computer-generated hologram is arranged to form structured light having a corresponding pattern within the scene. The holographic controller is further arranged to change the pattern of the structured light formed by at least one of the plurality of computer-generated holograms.