Provided herein are methods of amplifying nucleic acids. In particular, methods are provided for amplifying circular RNA molecules. In certain embodiments, circular DNA molecules for amplification are generated from circular RNA molecules.

A post-surgery brassiere comprises at least two catches for engaging two ring assemblies that detachably couple to collection bulbs and detachably couple to the two catches.

A brassiere includes bra cups holding a wearer's breasts. A shaping foam layer is sandwiched between outer and inner layers of each bra cup and extends from a lower to an upper edge of each cup. A support shelf assembly spans across a lower portion of each bra cup and includes a support component located proximate the lower edge of the cups, a non-stretch stabilizer fabric layer spanning between both cups and along a full length of the support component, and a supportive foam layer spanning between both cups and along the support component. The inner, outer, shaping foam, stabilizer fabric, and supportive foam layers span across a center gore that connects the bra cups to form a front panel of the brassiere. The support shelf assembly is molded between the inner layer and the shaping foam layer and is an embedded, integral part of the front panel.

A brassiere includes bra cups holding a wearer's breasts. A shaping foam layer is sandwiched between outer and inner layers of each bra cup and extends from a lower to an upper edge of each cup. A support shelf assembly spans across a lower portion of each bra cup and includes a support component located proximate the lower edge of the cups, a non-stretch stabilizer fabric layer spanning between both cups and along a full length of the support component, and a supportive foam layer spanning between both cups and along the support component. The inner, outer, shaping foam, stabilizer fabric, and supportive foam layers span across a center gore that connects the bra cups to form a front panel of the brassiere. The support shelf assembly is molded between the inner layer and the shaping foam layer and is an embedded, integral part of the front panel.

A brassiere and method of making including a front portion configured to receive the breasts of the wearer and a back panel including a first lateral side affixed to the front portion and a second lateral side affixed to the front portion. The back panel includes an inner fabric layer configured to contact the wearer's skin, an outer fabric layer opposing the inner fabric layer, and reinforcing panels positioned between the inner fabric layer and the outer fabric layer. A first reinforcing panel extends from the first lateral side, a second reinforcing panel extends from a second lateral side and a third reinforcing panel extends between, but not in contact with, the first and second reinforcing panels.

A brassiere and method of making including a front portion configured to receive the breasts of the wearer and a back panel including a first lateral side affixed to the front portion and a second lateral side affixed to the front portion. The back panel includes an inner fabric layer configured to contact the wearer's skin, an outer fabric layer opposing the inner fabric layer, and reinforcing panels positioned between the inner fabric layer and the outer fabric layer. A first reinforcing panel extends from the first lateral side, a second reinforcing panel extends from a second lateral side and a third reinforcing panel extends between, but not in contact with, the first and second reinforcing panels.

A nursing garment computing system includes a nursing garment having a pair of breast cups with an electronic sensor to indicate the status of a breast in the pair of breast cups and a computing device to record the sensor data indicative of whether the breast cup is open or closed. The data can be electronically sent to an external device such as a cell phone or personal digital assistant to keep track of which breast cup has been opened for breastfeeding event, time between breastfeeding event, amount of time for breastfeeding from each side of the breast, a record of which side was nursed last, and a record of which side was nursed first during the last breastfeeding event.

A nursing garment computing system includes a nursing garment having a pair of breast cups with an electronic sensor to indicate the status of a breast in the pair of breast cups and a computing device to record the sensor data indicative of whether the breast cup is open or closed. The data can be electronically sent to an external device such as a cell phone or personal digital assistant to keep track of which breast cup has been opened for breastfeeding event, time between breastfeeding event, amount of time for breastfeeding from each side of the breast, a record of which side was nursed last, and a record of which side was nursed first during the last breastfeeding event.

The front enclosure adjustable bra of the present invention includes an adjustment feature that allows adjustment of the length of the front, adjustment of the length of the side and adjustment of the length of the back, and/or adjustment of the cup size. A bra comprises of a pair (two) of bra cups to hold the breasts and a band reaching around the torso to secure the bra in place. The band comprises of a front under bust band attached to and underlying the bra cups, with a front enclosure or not, a back band comprising of two bands that meet at the centre with a buckle and fold out with hooks (or buckles & tunnels) on each side, right and left, a length adjustment feature for independently adjusting the length of the band conducive to the need of the wearer. Or this may be a band in the middle of the back with two buckles, one on each side. This adjustment allows for it to be bigger or smaller and may be adjusted throughout time of the wearer according to their needs.

A post-surgery medical bra garment is described for implant based, breast constructions, and reconstructions involving mastectomies, and mammoplasty procedures constructed on symmetrical (left and right) elastic power-net fabric panels.