Compositions, devices, and systems comprising a corneal tissue carrier, wherein the corneal tissue carrier comprises a corneal tissue sample and a fluid; wherein the fluid comprises resazurin. Additionally, methods including measuring cell viability.

The invention provides an isolated dehydrated corneal tissue, comprising a full thickness corneal stroma, and substantially all, or all, of the Bowman's membrane, wherein the stroma contains cellular material. Also provided is a method to product the tissue and uses of the tissue.

A multilayer cornea harvesting apparatus capable of obtaining layers of cornea from one cornea enucleation object and a cornea harvesting method is disclosed. The apparatus includes a base supported on a bottom surface, a mount detachably coupled to the base, allowing a cornea enucleation object to be placed thereon, having a first opening part formed at a portion corresponding to the cornea of the cornea enucleation object, and having a first medicinal fluid charging space formed between the base and the mount, a stabilizer fixing, to the mount, a sclera portion of the cornea enucleation object placed on the mount and having a second opening part formed at a portion corresponding to the cornea, and an upper cover detachably provided on the upper side of the cornea, supported by the mount or supporting the mount, and forming a second medicinal fluid charging space on the upper side of the cornea.

A method for performing a corneal transplantation comprising the steps of: inserting at least part of a corneal implant into the anterior chamber of the eye, the corneal implant comprising an implant portion and a manipulating portion, the manipulating portion comprising non-endothelial tissue; positioning the implant portion of the implant to adhere to the posterior surface of the cornea using the manipulating portion.

Corneal implant (1) designed for correcting irregularities of the corneal curvature of a subject, the implant (1) having a generally dome-shaped structural body (2) configured to impose a regular curvature to the corneal portions designed to be in contact with the implant, wherein the structural body comprises an outer peripheral ring (10) and an inner reticular structure (20), wherein the inner reticular structure (20) comprises at least one first and one second series of beams (21, 22) intersecting each other, the beams (21) of the first series having a respective first end (31) connected to the outer peripheral ring (10), wherein the total area of void portions (24) within the meshes of the reticular structure (20) is between 50 and 99.9% of the surface area of the reticular structure (20).

A structured artificial construct that allows corneal endothelium to be regenerated from isolated cells outside the human or animal body is provided. The structured artificial construct is formed from a dome-shaped base body with a honeycomb structure formed in a concave side of the base body. Methods for generating the structured artificial construct are also provided.

A corneal implant designed for correcting irregularities of the corneal curvature of a subject, the implant having a dome-shaped structural body configured to impose a regular curvature to the corneal portions designed to be in contact with the implant. The structural body includes an outer peripheral ring and an inner reticular structure. The inner reticular structure includes at least one first and one second series of beams intersecting each other. The beams of the first series have a respective first end connected to the outer peripheral ring, wherein the total area of void portions within the meshes of the reticular structure is between 50 and 99.9% of the surface area of the reticular structure, wherein the beams of both the first and second series have both ends connected to the outer peripheral ring.

The present solution can temporarily impart the handling characteristics of corneal stroma to the otherwise very thin, flimsy, coiling, and fragile Descemet membrane endothelial keratoplasty (DMEK) tissue during its insertion into the anterior chamber and positioning in apposition against the cornea of the recipient eye. The device of the present solution can be configured in a number of ways. In a first configuration, a scaffold can be coupled with the endothelial side of the DMEK graft. In a second configuration, the scaffold can be coupled with the stromal side of the DMEK graft. In a third configuration, one or more scaffolds can be coupled with both the endothelial and stromal side of the DMEK graft.

A technique can consistently achieve thicknesses of ≤ 50 .Math.m for corneal tissue for for Descemet stripping automated endothelial keratoplasty (DSAEK). Grafts with thicknesses of ≤ 50 .Math.m are also known as nanothin DSAEK (NT-DSAEK) grafts. Evidence shows that using thinner DSAEK grafts, particularly NT-DSAEK grafts, can significantly improve visual outcomes. According to an example embodiment, a method for producing a corneal graft includes drying a donor cornea to cause a pre-cut thickness of the donor cornea to decrease. The method includes, concurrently with drying the donor cornea, determining pre-cut thickness measurements for the donor cornea. The method includes, in response to the precut thickness measurements indicating the pre-cut thickness of the donor cornea has decreased to a predetermined value, cutting the donor cornea to a post-cut thickness of ≤ 100 .Math.m, or more particularly ≤ 50 .Math.m, to produce a corneal graft.

A method for fabricating a cornea includes affixing a frame to at least one cell culture insert comprising a generally cylindrical structure having a proximal end and a distal end, a base disposed at the proximal end, and a porous membrane disposed between the proximal end and the distal end; affixing a dome-shaped member to the porous membrane within the frame, the dome-shaped member comprising a crown, a dome base, and a surface connecting the crown and the dome base; depositing a material comprising a matrix-forming compound on the frame such that the crown and at least a portion of the surface of the dome-shaped member is coated with the material comprising the matrix-forming compound; and removing the dome-shaped member to produce a fabricated cornea attached to the frame. A system for fabricating a cornea and a cornea scaffold are also described herein.