A method for characterizing cancer organoid response to an immune cell based therapy, includes providing a panel of different combinations of cancer organoid cells and immune cells to culturing wells and culturing the different combination under conditions that support organoid growth. Brightfield and corresponding fluorescence images of the culturing wells are captured and provided to one or more trained machine learning algorithms that identify and distinguish cancer organoid cells from immune cells and characterize cancer organoid morphology changes caused by an immune cell based therapies, from which an analytical report including a characterization of cancer organoid cell death caused by the immune cell based therapy is provided.

The method of the present invention classifies the nuclei in prostate tissue images with a trained deep learning network and uses said nuclear classification to classify regions, such as glandular regions, according to their malignancy grade. The method according to the present disclosure also trains a deep learning network to identify the category of each nucleus in prostate tissue image data, said category representing the malignancy grade of the tissue surrounding the nuclei. The method of the present disclosure automatically segments the glands and identifies the nuclei in a prostate tissue data set. Said segmented glands are assigned a category by at least one domain expert, and said category is then used to automatically assign a category to each nucleus corresponding to the category of said nucleus' surrounding tissue. A multitude of windows, each said window surrounding a nucleus, comprises the training data for the deep learning network.

This invention makes it possible to build an image identification model having high accuracy of identification using divided training images into which training images are divided. An image identification system includes: an image dividing unit which divides training images of a first training data set and assigns a label assigned to a training image from which dividing occurs to the divided training images as tentative labels; a texture index computing unit which computes texture indexes for each of the divided training images; a tentative label prediction model building unit which builds a tentative label prediction model to predict tentative labels assigned to the divided training images based on the texture indexes; and a label comparison unit which compares first tentative labels assigned to the divided training images with second tentative labels predicted with respect to the divided training images by the tentative label prediction model and extracts divided training images for which there is discrepancy between the first and second tentative labels as those images for which it is highly necessary to modify tentative labels.

A system and method for de novo drug discovery using machine learning algorithms. In a preferred embodiment, de novo drug discovery is performed via data enrichment and interpolation/perturbation of molecule models within the latent space, wherein molecules with certain characteristics can be generated and tested in relation to one or more targeted receptors. Filtering methods may be used to determine active novel molecules by filtering out non-active molecules and contain activity predictors to better navigate the molecule-receptor domain. The system may comprise neural networks trained to reconstruct known ligand-receptors pairs and from the reconstruction model interpolate and perturb the model such that novel and unique molecules are discovered. A second preferred embodiment trains a variational autoencoder coupled with a bioactivity model to predict molecules exhibiting a range of desired properties.

In some embodiments, a process and system are provided for generating a user interface for classification of a sample image of a cell that includes receiving a sample image of a sample particle from a biological sample and selecting reference images that each portray a reference particle of a biological sample. The reference images can be ordered based on similarity and the reference images can be selected based on the order. The first selected reference image can be aligned with the sample image and expanded such that the adjacent edges of the reference image and sample image are the same. The expanded image can be dynamically filled. The sample image and the expanded reference image can be displayed in a user interface.

A method for classifying images of oligolayer exfoliation attempts. In some embodiments, the method includes forming a micrograph of a surface, and classifying the micrograph into one of a plurality of categories. The categories may include a first category, consisting of micrographs including at least one oligolayer flake, and a second category, consisting of micrographs including no oligolayer flakes, the classifying comprising classifying the micrograph with a neural network.

Disclosed is a method for diagnosing a neurodegenerative disease in a subject. The method comprises obtaining from the subject a sample comprising at least one live blood cell, and optionally isolating at least one live blood cell from the sample. The method further comprises generating one or more multispectral or hyperspectral images of the at least one cell, and analysing spectral characteristics of autofluorescence from the at least one cell. Also disclosed is a system configured to aid in the detection or diagnosis of a neurodegenerative disease. Also disclosed is a method for selecting a subject for treatment for a neurodegenerative disease. Also disclosed is a method for monitoring the response of a subject to a therapeutic treatment for a neurodegenerative disease. Also disclosed is a protocol for monitoring the efficacy of a therapeutic treatment for a neurodegenerative disease.

A reference image and at least one object image and at least one analysis image are used in a method for analyzing a structure within a fluidic system. A reference image section with the structure to be analyzed, which is isolated from a reference image, is provided, the reference image having been recorded with a first camera setting. An object image which has the same fluidic state as the reference image and which was recorded with the first or a second camera setting is selected. Using the object image and using the reference image section, an image registration is performed and an edge recognition is applied for the purposes of creating a mask. At least one analysis image is selected beforehand or afterwards, the at least one analysis image and the object image having been recorded with the same camera setting. The mask is applied to the analysis image for the purposes of isolating the image section of the analysis image to be analyzed. Subsequently, the image section to be analyzed can be examined using an image-analytical evaluation.

A method, computer program, and computer system is provided for predicting data from an image. An image is divided into one or more patch images. Spatial features corresponding to the one or more patch images are compressed. Output data corresponding to the compressed spatial features is predicted. The output data is predicted based on minimizing one or more loss functions corresponding to the compressed spatial features.

A system for typing biological cells includes a tunable Fabry-Perot etalon, and imaging sensor, and a processor. The imaging sensor acquires one or more images of one or more biological cells from light transmitted through the tunable Fabry-Perot etalon. Each image represents signal associated with one or more wavelengths transmitted through the tunable Fabry-Perot etalon. The processor is configured to determine a type of each of the one or more biological cells. Determining the type uses a machine learning algorithm and is based at least in part on one or more of an image segmentation, a patch extraction, a feature extraction, a feature compression, a deep feature extraction, a feature fusion, a feature classification, and a prediction map reconstruction.