Systems for treating a subject's tissue can include a thermally conductive cup, a tissue-receiving cavity, and a vacuum port. The vacuum port is in fluid communication with the tissue-receiving cavity to provide a vacuum for drawing the submental tissue, or other targeted tissue, into the tissue-receiving cavity. A thermal device can cool and/or heat the conductive cup such that the conductive cup non-invasively controls the temperature of subcutaneous lipid-rich cells in the tissue. A restraint apparatus can hold a the conductive cup in thermal contact with the target region.

Systems for treating a subject's tissue can include a thermally conductive cup, a sealing member, and/or a liner assembly. The systems can include an applicator capable of being reconfigured for a particular treatment site. Components of the applicator can be replaced to achieve a desired configuration. The replaceable components can include contoured heads, liners, and/or sensors. The applicator can draw a vacuum to install various components and/or draw tissue into the applicator. The applicator can cool and/or heat the tissue to affect targeted tissue.

Disclosed herein are cryoablation devices and methods of using the same, including to ablate gallbladders. In certain implementations, the device is a catheter that has an expandable cryogen balloon for expansion within a gallbladder and at least one suction opening for applying suction within the gallbladder. According to other implementations, the device has a cryogen probe and a suction catheter slidably positioned on the probe.

The cooling system (100, 300, 400, 500, 700, 750) includes an applicator (101, 501, 701) configured to hold a predetermined amount of solid coolant and fluid coolant (117) to cool a targeted area of the body (162, 570) to crystalize the lipid-rich cells underneath the targeted area to reduce the fat cells. The applicator may include a thermoelectric cooler (TEC, 136, 704, 706) where the hot side is cooled by coolant held within the applicator. The cold side of the TEC may be thermally coupled to a cooling plate (138, 560) configured to cool a targeted area of the skin at a predetermined temperature range for a predetermined period of time. The cooling system may also be used to relieve localized pain at certain area of the body and/or utilized for cryotherapy.

Systems and methods for a medical device configured to provide cooling to a tissue region are provided. The medical device may be configured to noninvasively cool the tissue region to a predetermined operating temperature via a two-phase heat transfer process. In some aspects, the medical device can be configured to be in communication with a vacuum to provide a suction to the medical device. In some aspects, the medical device can be adapted to a medical device to noninvasively cool the tissue region near a fractional treatment area.

A system and method of monitoring, controlling and/or detecting events during the removal of heat from subcutaneous lipid-rich tissue is described. In some examples, the system detects an increase in temperature at a treatment device in contact with the skin of a subject, determines that the increase in temperature is related to a treatment event, and performs an action based on the determination. In some examples, the system shuts off the treatment device, alerts an operator, or reduces the cooling in response to a determined treatment event.

The present disclosure refers to a non-shivering cryothermogenesis method for locally reducing adipose tissue of a subject's body, including: (a) applying at least one applicator to the subject's skin; (b) applying at least one additional applicator to a local region wherein adipose tissue reduction is desired; (c) the applicator maintaining skin superficial temperature between 4 C. and 12 C.; and (d) applying the applicators for a total period of time between 20 and 60 min.

A method and apparatus in accordance with a particular embodiment of the present invention includes applying adhesive onto skin of a human subject. An applicator is then brought into contact with the adhesive such the adhesive is disposed between the applicator and the subject's skin. The applicator is activated to cool a tissue region via the subject's skin, via the heat-transfer surface of the applicator, and via the adhesive. While the tissue region cools, the adhesive also cools, thereby reversibly strengthening adhesion between the subject's skin and the heat-transfer surface and forming a strong bond therebetween. The strengthened adhesion inhibits any movement of the applicator relative to the skin. After cooling the tissue region, the adhesive is warmed, thereby weakening the adhesion which allows the heat-transfer surface of the applicator to be easily separated from the skin.

Compositions and formulations having one or more anti-freeze proteins for use with devices and systems that enable tissue cooling, such as cryotherapy applications, for alteration and reduction of adipose tissue are described. Embodiments of the technology are further directed to methods, compositions having one or more anti-freeze proteins and devices that protect non-targeted cells from freeze damage during dermatological and related aesthetic procedures requiring sustained exposure to cold temperatures. Further embodiments of the technology include systems for enhancing sustained and/or replenishing release of a freezing point depressant having one or more anti-freeze proteins to a treatment site prior to and during cooling applications.

Systems for treating a subject's tissue can include a thermally conductive cup, a tissue-receiving cavity, and a vacuum port. The vacuum port is in fluid communication with the tissue-receiving cavity to provide a vacuum for drawing the submental tissue, or other targeted tissue, into the tissue-receiving cavity. A thermal device can cool and/or heat the conductive cup such that the conductive cup non-invasively controls the temperature of subcutaneous lipid-rich cells in the tissue. A restraint apparatus can hold a the conductive cup in thermal contact with the target region.