A system for a high resolution optical spectrum analyzer (OSA) using an efficient multi-pass configuration is disclosed. The system may include an entrance slit to allow inward passage of an optical beam. The system may also include a grating element to diffract the optical beam. The system may further include a retroreflective element to retroreflect the optical beam. The system may also include a mirror to reflect the optical beam. The system may include an exit slit, which in some examples may be adjacent to the entrance slit. The exit slit may allow outward passage of the optical beam for a high resolution optical measurement.

A spectroscopic system may include: a probe having a probe tip and an optical coupler, the optical coupler including an emitting fiber group and first and second receiving fiber groups, each fiber group having a first end and a second end, wherein the first ends of the fiber groups are formed into a bundle and optically exposed through the probe tip; a light source optically coupled to the second end of the emitting fiber group, the light source emitting light in at least a first waveband and a second waveband, the second waveband being different from the first waveband; a first spectrometer optically coupled to the second end of the first receiving fiber group and configured to process light in the first waveband; and a second spectrometer optically coupled to the second end of the second receiving fiber group and configured to process light in the second waveband.

A measurement system includes an optical source (e.g., laser) to irradiate a sample (e.g., a cell); a solid-state photon detector (SSPD) to receive resultant light from the sample; and a photon counter to count photons received by the SSPD. The photon counter can include a differentiator to provide a differentiated photon signal and a crossing detector configured to count photons based on a number of times the differentiated photon signal crosses a predetermined threshold level. In some examples, a pulse detector can provide a pulse-width signal from the SSPD output photon signal, and a pulse counter can count based on both a number of pulses and widths of the pulses. The SSPD can include a silicon photomultiplier (SiPM) array or a solid-state photomultiplier. Some examples use the measurement system to measure samples in fluids, e.g., in flow cytometers or multi-well plates.

A system for a high resolution optical spectrum analyzer (OSA) using an efficient multi-pass configuration is disclosed. The system may include an entrance slit to allow inward passage of an optical beam. The system may also include a grating element to diffract the optical beam. The system may further include a retroreflective element to retroreflect the optical beam. The system may also include a mirror to reflect the optical beam. The system may include an exit slit, which in some examples may be adjacent to the entrance slit. The exit slit may allow outward passage of the optical beam for a high resolution optical measurement.

The invention provides methods and apparatus comprising a multi-wavelength laser source that uses a single unfocused pulse of a low intensity but high power laser over a large sample area to collect Raman scattered collimated light, which is then Rayleigh filtered and focused using a singlet lens into a stacked fiber bundle connected to a customized spectrograph, which separates the individual spectra from the scattered wavelengths using a hybrid diffraction grating for collection onto spectra-specific sections of an array photodetector to measure spectral intensity and thereby identify one or more compounds in the sample.

Apparatus for measuring spectra from one or more samples, the apparatus including a reference waveguide that receives illuminating radiation used to illuminate at least one sample, at least one sample waveguide that receives sample radiation at least one of reflected from and transmitted through a respective sample, an optical system that spatially distributes radiation from each of the waveguides based on a frequency of the radiation, and focuses radiation from the optical fibres into an imaging plane and an imaging device that captures an image of the focused and spatially distributed radiation from the imaging plane so that the image includes respective spectra from each of the waveguides.

Problem: To provide an observation auxiliary device that can appropriately and readily perform observation using an exciting light as a light source.

Resolution Means: Provided are an imaging unit 104 that uses a light emitted from a second beam splitter 202 of a microscope 2 that can use an exciting light and an observation light, which is a light including a wavelength other than that of the exciting light, as a light source by switching there between and is provided with the second beam splitter 202 to image images of the same observation region of the microscope 2 in situations where the exciting light and the observation light are used as the light source and an output unit 106 that overlaps, synthesizes, and outputs the images imaged by the imaging unit 104 respectively using the exciting light and the observation light as the light source.

A device comprising a circuitry configured to obtain a sequence of digital images from an image sensor; select a region of interest within a digital image of the sequence of digital images; perform motion compensation on the region of interest to obtain a motion compensated region of interest based on motion information obtained from the sequence of digital images and a predefined accumulated time interval; define a mask pattern based on the compensated region of interest; apply the mask pattern to an electronic light valve.

A two-dimensional spectrometer includes a first mirror, a prism, a diffraction grating, a lens, a second mirror, and a two-dimensional sensor. The first mirror is configured to receive the optical signal from the optical entrance and reflect the optical signal towards the prism. After passing through the prism, the optical signal is provided to the diffraction grating. The diffraction grating diffracts the optical signal so as to generate a diffracted optical signal which is directed back through to prism, wherein the lens configured focuses the diffracted optical signal onto the second mirror. The second mirror reflects the diffracted optical signal back through the lens which focuses the diffracted optical signal onto the two-dimensional sensor. The diffraction grating may be an echelle grating.

An optical system has an illumination optical assembly, a detection optical assembly, a wavefront shaping device, and a controller. The illumination optical assembly focuses interrogating optical radiation to a focal point on or in a sample. The interrogating optical radiation propagates to the focal point along a first optical axis. The detection optical assembly direct optical radiation emanating from the focal point to a detector. The emanating optical radiation propagates from the focal point along a second optical axis. The wavefront shaping device is disposed in an optical path of the interrogating optical radiation or in an optical path of the emanating optical radiation. The controller sets a configuration of the wavefront shaping device to correct for aberration. The first optical axis is at a non-zero angle with respect to the second optical axis.