The invention provides a method termed protein retention ExM (proExM), in which proteins, rather than labels, are anchored to the swellable gel, using a cross-linking molecule. This proExM strategy can be used to perform nanoscale imaging of immunostained cells and tissues as well as samples expressing various FPs as fluorescent signals from genetically encoded fluorescent proteins and/or conventional fluorescently labeled secondary antibodies and streptavidin that are directly anchored to the gel are preserved even when subjected to the nonspecific proteolytic digestion.

The present disclosure provides methods for carrying out Romanowsky-type stains, specifically Wright-Giemsa and May-Grnwald stains, quickly and efficiently. The methods greatly reduce the overall amount of time required to complete a Wright-Giemsa stain or a May-Grnwald stain of sufficient quality on a biological sample. The subject methods can be applied to both manual and automated staining procedures.

The invention refers to a composition for the fixation of histological preparations, comprising a solution of glyoxal, wherefrom acids, normally present in the commercially available glyoxal, have been removed. The composition of the invention provides an optimal fixative for the preservation of structure, antigenic components and nucleic acids in tissues. The acid-free glyoxal has limited toxicity, is not considered as a carcinogenic agent and represents a valid alternative to formalin for the fixation of tissues and cells.

A clearing agent and mounting solution for microscopy is disclosed comprising at least trichloroethanol and/or derivatives thereof, where the refractive index of the solution is greater than or equal to about 1.3810. Also disclosed are methods of preparing specimens for microscopy.

The present invention relates to the technical field of computers, and more particularly, to a method for generating training data based on immunohistochemistry, and a storage device. The method for generating the training data based on immunohistochemistry includes the following steps: performing immunohistochemical staining on a target object through different antibodies; labeling the target object according to staining results; and generating training data according to labeling results. According to the method, a pathologist can label a tissue or cell of interest rapidly and conveniently without performing labeling through H&E slicing in combination with histomorphology. In addition, since this tissue or cell is labeled based on an immunohistochemistry technology of a gold standard, this labeling is more accurate compared with manual labeling performed through H&E slicing in combination with histomorphology.

The present disclosure describes a composition for preparing histological, postmortem, cytological or similar samples for the analysis thereof, wherein the composition is not harmful, non-toxic, and able to rapidly produce dehydration and diaphonization of the biological samples.

A clearing agent and mounting solution for microscopy is disclosed comprising (a) trichloroethanol, (b) optionally, trichloroacetic acid, (c) optionally, glycerol and (d) optionally, water, where the refractive index of the solution is greater than or equal to about 1.3810. The solution can further comprise a C1-C6 alcohol, other acids, and/or a stain. The solution can also comprise derivatives and/or analogs of 2,2,2-trichloroethanol and/or trichloroacetic acid. Also disclosed is a method of preparing specimens for microscopy comprising (a) applying a specimen to a microscope slide or a cuvette, (b) applying a quantity of the above solution sufficient to mount the specimen, and (c) optionally applying a cover slip. The solution can be used effectively with stains or dyes, and with fresh, partially dry or dried materials, and for temporary or semi-permanent to permanent mounting. The solution can be used with specimens or tissues/cells/parts originating from animals, poultry, livestock, humans, higher plants, yeasts, molds, microorganisms, insects, mites, or reptiles.

The present disclosure provides methods for carrying out Romanowsky-type stains, specifically Wright-Giemsa and May-Grnwald stains, quickly and efficiently. The methods greatly reduce the overall amount of time required to complete a Wright-Giemsa stain or a May-Grnwald stain of sufficient quality on a biological sample. The subject methods can be applied to both manual and automated staining procedures.

Object of the invention is a novel fixative composition for samples of biological material that is non-toxic and that can also easily and safely be used in big quantities.

The present disclosure provides compositions and methods for using fixed biological samples in partition-based assays. In at least one embodiment, the disclosure provides a composition comprising a fixed biological sample and an un-fixing agent contained in a partition, such as a discrete droplet. In some embodiments, the disclosure provides un-fixing agent compounds capable of catalytically cleaving crosslinks in fixed biological samples, particularly crosslinked nucleic acids, such as RNA.