An apparatus is disclosed for separating and preserving biomolecules of a biological fluid sample. The apparatus includes an assembly having sides forming a hollow shape having a first opening at one end and second opening at the opposite end, a sample mixing chamber positioned adjacent the first opening within the assembly, the sample mixing chamber from which a flow of the biological fluid sample is actuated in a direction from the sample mixing chamber to the first matrix layer, the sample mixing chamber being in a direction downstream of the first opening, a first valve positioned between the sample mixing chamber and the first matrix layer, the first valve configured to control the flow to the first matrix layer, a first input in fluid communication with the sample mixing chamber and positioned upstream of the first valve, a second input positioned between the first matrix layer and the second matrix layer, and a second valve positioned between the second matrix layer and the second opening, the second valve configured to control the flow to the second matrix layer.

A target analyte is extracted out of a sample fluid in a sample fluid passage by diffusing the target analyte through a supported liquid membrane to a product fluid passage. Extraction of the target analyte is accelerated by applying an electric field across and perpendicular to the supported liquid membrane with electrodes. Passage of selected ions across an exchange membrane extending between one of the electrodes and the supported liquid membrane is inhibited.

Disclosed herein is a preprocessing apparatus that makes it possible to highly efficiently analyze specimens held by solid media, such as dried blood spots to be used for newborn mass screening or the like. The preprocessing apparatus includes: a preprocessing container setting part in which a preprocessing container containing a solid sample including a specimen to be analyzed and a solid medium holding the specimen is to be set; a carrying mechanism that carries the preprocessing container set in the preprocessing container setting part; and a preprocessing part that has a port for setting the preprocessing container carried by the carrying mechanism and that is configured to perform preprocessing including extraction processing for extracting the specimen from the solid sample contained in the preprocessing container set in the port.

A method for detecting albumin based on a colorimetric assay and a system thereof are disclosed. Gold nanoparticles are added into the sample preparing device having a sample without spectroscopic tags, wherein the sample without spectroscopic tags is formed as the alkaline solution to avoid the interference substances adhering on the gold nanoparticles. The gold nanoparticles are concentrated by using the microfluidic concentrator with the circular ion exchange membrane by applying an external electric field across two electrodes. The image of the concentrated gold nanoparticles is captured by the image capturing device for measuring the saturation intensities of the image, wherein there is a relation between the saturation intensities and the concentration of the albumin in the sample without spectroscopic tags. The concentration of the albumin of the sample without spectroscopic tags is obtained by the relation and the measured saturation intensities.

The present invention relates to a process for the extraction of analyte compounds from a sample comprising one or more analytes in a donor phase into an acceptor phase, comprising the steps of: a) providing an electrically conductive donor phase comprising the compounds in a first electrically conductive solvent or solvent blend, and an electrode arranged in electrically conductive contact with the donor phase, b) providing an electrically conductive acceptor phase in electrically conductive contact with a second electrode; and c) providing an insulator phase in fluid communication with at least one of the donor phase and the acceptor phase, wherein the insulator phase is immiscible with the donor phase and/or the acceptor phase, and d) (d) applying an electrical field between the first and the second electrode.

A method for detecting albumin based on a colorimetric assay and a system thereof are disclosed. Gold nanoparticles are added into the sample preparing device having a sample without spectroscopic tags, wherein the sample without spectroscopic tags is formed as the alkaline solution to avoid the interference substances adhering on the gold nanoparticles. The gold nanoparticles are concentrated by using the microfluidic concentrator with the circular ion exchange membrane by applying an external electric field across two electrodes. The image of the concentrated gold nanoparticles is captured by the image capturing device for measuring the saturation intensities of the image, wherein there is a relation between the saturation intensities and the concentration of the albumin in the sample without spectroscopic tags. The concentration of the albumin of the sample without spectroscopic tags is obtained by the relation and the measured saturation intensities.

A preprocessing apparatus uses preprocessing kits prepared for samples, respectively, sets the preprocessing kits in plural processing ports provided so as to correspond to preprocessing items, and executes a predetermined preprocessing item. A controller which controls operations of a carrying mechanism holding and carrying the preprocessing kits, and processing parts executing the preprocessing items in the respective processing ports, includes a processing-state control part, a random access part, and a preprocessing part. The random access part is configured to check availability of a preprocessing port corresponding to a preprocessing item to be executed on a sample contained in a preprocessing kit, and set the preprocessing kit in a processing port if the processing port is available as a processing port corresponding to the preprocessing item. The preprocessing part executes a corresponding preprocessing item on a sample in a preprocessing kit when the preprocessing kit is set in the processing port.

Disclosed are methods, compositions, and devices for an integrated, heterogenerous ion-exchange membrane-based plastic microfluidic biochip platform that can be used to detect multiple diagnostic markers present in real samples. Its various components can be easily integrated in a modular fashion for different applications. Automated control allows sequential and dynamic activation of different components on the chip. The integrated platform consists of three units and is designed to execute the following functions: (i) separation of the target biomolecules from the real sample, (ii) localizing and concentrating the targeted molecules at a specific location in the microfluidic chip, and (iii) detection of the targeted molecules using hybridization/docking events against a complementary ssDNA oligo-probe sequence or a specific antibody.

A kit for extracting mycotoxin residues in agricultural products according to the present disclosure includes a pipe, a first powder mixture layer and a second powder mixture layer. The pipe has an output port at the bottom thereof and an input port at the top thereof for inputting a sample solution. The first powder mixture layer is in the form of powder and filled in the pipe. The first powder mixture layer contains cation exchange resin powder, C18 and diatomaceous earth powder. The second powder mixture layer is in the form of powder and filled in the pipe. The second powder mixture layer is located below the first powder mixture layer and above the output port. The second powder mixture layer contains PSA powder, anhydrous magnesium sulfate powder, activated carbon, PLS powder, diatomaceous earth powder and C18 powder. The present disclosure further provides a method of obtaining a primary test liquid from agricultural products using the above kit.

An impurity acquisition system includes: an enrichment column that adsorbs impurities in a liquid being tested and a control unit that is used to switch between an enrichment process in which the liquid being tested is passed through the enrichment column, an eluent filling process in which a filling container is filled with a predetermined amount of eluent that elutes impurities adsorbed in the enrichment column, and an elution and collection process in which the eluent is passed through the enrichment column and collected in a collection container.