Systems and methods for uniquely identifying fluid-phase products by endowing them with fingerprints composed of dispersed colloidal particles, and by reading out those fingerprints on demand using Total Holographic Characterization. A library of chemically inert colloidal particles is developed that can be dispersed into soft materials, the stoichiometry of the mixture encoding user-specified information, including information about the host material. Encoded information then can be recovered by high-speed analysis of holographic microscopy images of the dispersed particles. Specifically, holograms of individual colloidal spheres are analyzed with predictions of the theory of light scattering to measure each sphere's radius and refractive index, thereby building up the distribution of particle properties one particle at a time. A complete analysis of a colloidal fingerprint requires several thousand single-particle holograms and can be completed in ten minutes.

Various embodiments are directed to a collection media assembly for receiving one or more particles from a volume of fluid within a fluid composition sensor. In various embodiments, the collection media assembly comprises a housing, a transparent substrate, a collection media disposed upon the transparent substrate and configured to receive one or more particles from a volume of fluid received through a fluid inlet; and at least one alignment feature. The housing defines an open lower end configured for interaction with an imaging device, such that the one or more particles received by the collection media are visible through the transparent substrate from the open lower end. Each of the at least one alignment features is configured to engage a corresponding element disposed within the fluid composition sensor so as to constrain relative movement between the collection media assembly and the corresponding element in at least a first direction.

Disclosed are optical interrogation apparatus that can produce lens-free images using an optoelectronic sensor array to generate a holographic image of sample objects, such as microorganisms in a sample. Also disclosed are methods of detecting and/or identifying microorganisms in a biological sample, such as microorganisms present in low levels. Also disclosed are methods of using systems to detect microorganisms in a biological sample, such as microorganisms present in low levels. In addition or as an alternative, the methods of using systems may identify microorganisms present in a sample and/or determine antimicrobial susceptibility of such microorganisms.

Various embodiments described herein relate to apparatuses and methods for detecting fluid particles and their characteristics. In various embodiments, a device for detecting fluid particles and their characteristics may comprise a lens free holographic microscope configured to collect fluid particles via inertial impaction. In various embodiments, the collection media may be replaceable within the apparatus. In various embodiments, the impactor nozzle may be selectively configured to avoid optical reflections and scattering from illumination light passing through the nozzle. Various embodiments are directed to a collection media assembly for receiving particles from a volume of fluid within a fluid composition sensor. A collection media assembly may comprise a collection media, an orifice, a seal engagement portion and a frame element configured to facilitate the serial use of a plurality of collection media assemblies within a fluid composition sensor.

The present disclosure presents systems, apparatuses, and methods of holographic imaging. In this regard, a method comprises transmitting light and illuminating a semi-transparent sample object; and forming, at a hologram plane, an interference pattern of a real image of the sample object from a scattered object beam and an unscattered reference beam from the transmitted light. To do so, the scattered object beam and the unscattered reference beam are in-line with one another, and a distance between the hologram plane to the sample object is set at a distance that substantially weakens a virtual image of the sample object formed from the scattered object beam and the unscattered reference beam. Accordingly, the method further comprises recording the interference pattern of a hologram formed from the scattered object beam and the unscattered reference beam at a detector; and reconstructing a 3D optical field of the hologram without phase retrieval.

Systems and methods for holographic characterization of protein aggregates. Size and refractive index of individual aggregates in a solution can be determined. Information regarding morphology and porosity can be extracted from holographic data.

Systems and methods for holographic characterization of protein aggregates. Size and refractive index of individual aggregates in a solution can be determined. Information regarding morphology and porosity can be extracted from holographic data.

Various embodiments are directed to a fluid composition sensor device and method of using the same. In various embodiments, the fluid flow composition sensor is configured to receive a volume of fluid, the fluid composition sensor comprising a housing, a removable fluid flow component, an impactor nozzle, a collection media assembly dock element configured to receive a replaceable collection media assembly comprising a collection media configured to receive one or more particles within the volume of fluid, an imaging device configured to capture an image of at least a portion of the one or more particles received by the fluid composition sensor, and a controller configured to determine, based at least in part on the image, at least one particle characteristic of the volume of fluid. The imaging device may be configured to capture the image of one or more particles received by the fluid composition sensor using lensless holography.

Systems and methods for holographic characterization of protein aggregates. Size and refractive index of individual aggregates in a solution can be determined. Information regarding morphology and porosity can be extracted from holographic data.

The invention is a method for estimating a representative volume of particles of interest (10 i) immersed in a sample, the sample extending in at least one plane, referred to as the sample plane (P 10), the sample comprising a sphering agent, capable of modifying the shape of the particles, the method comprising the following steps: a) illuminating the sample by means of a light source (11), the light source emitting an incident light wave (12) propagating towards the sample (10) along a propagation axis (Z); b) acquiring, by means of an image sensor (16), an image (I 0) of the sample (10), formed in a detection plane (P 0), the sample being arranged between the light source (11) and the image sensor (16), each image being representative of a light wave (14) referred to as an exposure light wave, to which the image sensor (16) is exposed under the effect of illumination; c) using the image of the sample (I 0), acquired during step b), and a holographic propagation operator, to calculate a complex expression (A (x, y, z)) of the exposure light wave (14) in different positions relative to the detection plane; the method comprising a step of estimating the representative volume (AA) of the particles of interest (10 i) depending on the complex expressions calculated during step c).