An active sieve device for the isolation and characterization of bio-analytes is provided, comprising a substrate for supporting the bio-analytes. The substrate comprises a plurality of interconnections and a plurality of regions, each region comprising a hole and at least one electrode embedded in or located on the substrate and electrically associated with the hole. Each region further comprises at least one transistor integrated in the substrate and operably connected to the at least one electrode and to at least one of the plurality of interconnections.

A pore device is used with a measurement device. The pore device includes a pore chip and a chip case which has a chamber partitioned by the pore chip. A measurement terminal group is provided to apply an electric signal from the measurement device to the chamber and output an electric signal generated in the chamber to the measurement device. Interface means is connected to a nonvolatile memory such that the nonvolatile memory is accessible from an outside of the pore device.

An outside opening of each aperture of a plurality of counting chambers for performing particle counting based on the electric resistance method is connected to suction pump through a confluent piping. Liquid supplying part supplies an additional liquid to the counting chamber side after completion of counting of counting chamber, so that the liquid level of sample liquid of counting chamber will not descend to aperture or a predetermined liquid level.

A method of determining a charge of at least one test particle (as herein defined), comprising: applying one of an electric current or a voltage across an aperture connecting two chambers, whereby the chambers are at least partially filled with electrolyte and whereby the at least one test particle is suspended in the electrolyte of at least one of the chambers; measuring a value indicative of the other of the electric current or voltage across the aperture; determining a time interval between a first and a second point in time, the second point in time corresponding to a point in time when the measured current or voltage has reached a specific proportion of the measured current or voltage at the first point in time; and determining the charge of the at least one test particle by: determining a value indicative of an electrical velocity component of a total velocity of at least one calibration particle having a known charge, taking into account that the total velocity of the at least one calibration particle comprises a non zero-convective velocity component and the electrical velocity component; determining a value indicative of an electrical velocity component of a total velocity of the at least one test particle, taking into account that the total velocity of the at least one test particle comprises a non-zero convective-velocity component and the electrical velocity component; and using the determined values indicative of the electrical velocity components of the test particle and the calibration particle to calibrate the quantitative relationship between the charge of the at least one test particle and the determined time interval.

Improved resolution and detection of nanoparticles are achieved when a nanopore connecting liquid compartments in a device running on the Coulter principle is provided with fluid coatings such as lipid walls. Fluid lipid walls are made of a lipid bilayer, and preferably include lipid anchored mobile ligands as part of the lipid bilayer. By varying the nature and concentration of the mobile ligand in the lipid bilayer, multifunctional coatings of lipids are provided.

A method of forming a plurality of lipid bilayers over an array of cells in a nanopore based sequencing chip is disclosed. Each of the cells comprises a well. A first salt buffer solution with a first osmolarity is flowed over a cell in the nanopore based sequencing chip to substantially fill a well in the cell with the first salt buffer solution. A lipid and solvent mixture is flowed over the cell to deposit a lipid membrane over the well that encloses the first salt buffer solution in the well. A second salt buffer solution with a second osmolarity is flowed above the well to reduce the thickness of the lipid membrane, wherein the second osmolarity is a lower osmolarity than the first osmolarity such that an osmotic imbalance is created between a first volume inside the well and a second volume outside the well.

A sensor for particle identification is provided. The subject sensor includes: a first chamber configured to be filled with an electrolytic solution; a first electrode provided inside the first chamber and configured to be connected to an external power supply for applying a voltage; a second chamber configured to be filled with the electrolytic solution; a second electrode provided inside the second chamber and configured to be connected to the external power supply; a data output means configured to output measurement data expressing an ion current generated between the first electrode and the second electrode; a partition separating the first chamber and the second chamber; and a presentation device for providing a unique identifier to an external computer device over a network.

A method of forming a plurality of lipid bilayers over an array of cells in a nanopore based sequencing chip is disclosed. Each of the cells comprises a well. A first salt buffer solution with a first osmolarity is flowed over a cell in the nanopore based sequencing chip to substantially fill a well in the cell with the first salt buffer solution. A lipid and solvent mixture is flowed over the cell to deposit a lipid membrane over the well that encloses the first salt buffer solution in the well. A second salt buffer solution with a second osmolarity is flowed above the well to reduce the thickness of the lipid membrane, wherein the second osmolarity is a lower osmolarity than the first osmolarity such that an osmotic imbalance is created between a first volume inside the well and a second volume outside the well.

An impedance cytometry device is described along with methods of accurately measuring particle size of particles contained in a fluid that is passed through the impedance cytometry device. The impedance cytometry device includes a substrate, and an electrode arrangement deposited on the substrate in a co-planar fashion. The electrode arrangement includes a drive electrode and a plurality of measurement electrodes located in a same plane as the drive electrode. The plurality of measurement electrodes includes at least two pairs of measurement sub-electrodes, each pair of measurement sub-electrodes including a first measurement sub-electrode positioned adjacent to the drive electrode, and a second measurement sub-electrode separated from the drive electrode by a respective first measurement sub-electrode. The impedance cytometry device may be incorporated into a substrate assembly of an electrowetting on dielectric (EWOD) device, such as in a substrate assembly containing electrowetting drive electrodes or a common reference electrode, or into a microfluidic blood counter device.

A flow cytometry system including a measuring chamber, an injection device arranged to inject a flow of biological particles to be analyzed in the measuring chamber, an evacuation device arranged to evacuate outside of the cytometry system the flow of biological particles injected in the measuring chamber, a measuring set arranged to measure at least one optical property of the biological particles to be analyzed, the measuring set including an emission device arranged to emit a light beam in the direction of the measuring chamber and capable of crossing the flow of biological particles, and at least one collecting device arranged to collect light rays coming from the measuring chamber, where the flow cytometry system further includes a support on which the injection device, the evacuation device, the emission device and the at least one collecting device are mounted.