Certain embodiments of the invention are directed to evaluating and identifying cells by recording and interpreting a time-dependent signal produced by unique cell respiration and permeability attributes of isolated viable cells.

Certain embodiments of the invention are directed to evaluating and identifying cells by recording and interpreting a time-dependent signal produced by unique cell respiration and permeability attributes of isolated viable cells.

A microparticle sorting apparatus includes a detection unit which detects microparticles flowing through a flow path; an imaging device which images a droplet containing the microparticles which is discharged from an orifice provided on an edge portion of the flow path; a charge unit which applies a charge to the droplets; and a control unit which determines a delay time as from a time that the microparticles are detected by the detection unit to the time at which the sum of intensity of an image region imaged by the imaging device reaches a maximum, making it possible for the charge unit to apply a charge to the microparticles once the delay time has lapsed after the microparticles are detected by the detection unit.

Disclosed herein are a particle sorting device capable of simply detecting bubbles, foreign substances, or the like in droplets, a method for analyzing particles, a program, and a particle sorting system. The particle sorting device includes a judgment unit, and the judgment unit judges whether or not captured image information including captured droplet image information about a brightness of an image of particle-containing droplets captured after discharge from an orifice has changed with respect to previously-set reference image information including reference droplet image information about a brightness of an image of droplets captured after discharge from the orifice.

The present disclosure provides a particle sorting apparatus, a particle sorting method, and a non-transitory computer-readable storage medium storing program that enable sorting object particles to be sorted with high precision, even when the sorting object particles are large. In the particle sorting apparatus, a charging unit that applies charges to at least a part of liquid droplets ejected from an orifice to generate a fluid stream and a charging control unit that adjusts a charge application end time in the charging unit according to sizes of particles included in the liquid droplets are provided.

Disclosed is a system for setting the timing or phase of the separation of droplets from a fluid stream in a flow cytometer, or the timing or phase of a charge pulse generator, based upon the collected charge of charged droplets. In one embodiment, a conductive mesh can be used to collect the charged droplets that are either deflected or not deflected by the deflection plates. In another embodiment, the charge can be collected from metal plates in the waste collection device. In addition, a defanning device is disclosed that allows substantially uniform deflection of charged cells.

The present technology provides a technology for stabilizing break-off timings. Therefore, according to the present technology, there is provided a microparticle analysis device or the like including at least: a flow path in which a fluid including a sample flow containing microparticles and a sheath flow flowing to contain the sample flow; a droplet formation unit configured to form a droplet in the fluid by imparting vibration to the fluid using a vibration element; an electric charge application unit configured to apply electric charge to a droplet containing the microparticles; an imaging unit configured to obtain a photo of a phase of a certain time; and a control unit configured to control a timing at which the droplet breaks off on a basis of the photo.

There is provided a droplet sorting device including a detector configured to detect a state of droplets ejected from an orifice that generates a fluid stream and of satellite droplets existing between the droplets, and a controller configured to control a frequency of a driving voltage supplied to a vibration element that applies vibration to the orifice on the basis of positions where the satellite droplets exist.

Certain embodiments of the invention are directed to evaluating and identifying cells by recording and interpreting a time-dependent signal produced by unique cell respiration and permeability attributes of isolated viable cells.

The present invention relates to a system comprising a device having a nozzle for dispensing liquid droplets on a collection surface mounted on a horizontally moveable tray, characterized in that the system comprises indicating means capable of indicating a target position on the collection surface where the liquid droplets are dispensed by said nozzle, said system further comprising a camera arranged to capture image data of said collection surface at said target position. The invention also relates to a method for dispensing at least one liquid droplet onto a desired position on a surface of a collection device and to the use of a device having a flat surface coated with a coating that alters its optical properties when in contact with aqueous medium in calibration of a system according to the invention.