An apparatus for analyzing particles in a solution includes a unit configured to place a flow cell having a flow path for flowing a sample solution containing the particles; a unit configured to illuminate the sample solution flowing through the flow path of the flow cell; a photodetector that detects a scattered light and/or fluorescence generated from the particles in the sample solution; and a unit configured to analyze the particles based on their signal intensities detected by the photodetector, wherein the flow cell has the flow path formed in a substrate, a reflection plane is formed on the side surface of the flow path, the reflection plane leads the lights generated in the flow path of the flow cell and advancing in the substrate in-plane direction to a specified region of the surface of the flow cell, and the photodetector detects the light exiting from the specified region to the outside.

A method for treating a biological sample, preferably a food sample which may contain one or more species of interest, including a step of decomplexification by acoustophoresis.

A method of detecting bioaerosols, including detecting particles and estimating a particle size for each detected particle and determining a fluorescence strength for each detected particle. Comparing particle size and fluorescence strength for each detected particle to arrive at a normalized fluorescence strength per particle and comparing normalized fluorescence strengths over a time period to a maximum threshold, to detect highly fluorescent manmade substances.

A particle detector that includes a housing defining a chamber, and an air stream injector, producing an airstream with entrained particles, in the chamber. A light source produces a light beam that intersects with and is wider than the air stream. A light detection assembly detects light generated by scattering of the light beam, by particles in the air stream. A digitizer produces a sequence of scattering digital values, each representing light detected per a first unit of time duration. Additionally, a summing assembly produces a sequence of summed scattering digital values, each equaling a sum of a sequential set of n of the digital values, and wherein successive summed digital values are offset by a the first unit of time duration and overlap by n1 of the first units of time duration with a nearest neighbor. Finally, a detection assembly processes the summed scattering digital values to detect particles.

A fluid handling and delivery system useful in generating a fluid stream in the flow path of microfluidic device.

Apparatus and methods for sample acquisition, including for example, samples for flow cytometry systems. Certain embodiments include a plurality of plates, valves, and conduits. In particular embodiments, the plates are stacked and the conduits extend through stack of plates, and in specific embodiments each valve is in fluid communication with a conduit.

A method of inseminating an animal including flowing a stream of a population of sperm cells through a channel, differentiating the sperm cells into two subpopulations of X-chromosome containing sperm cells and Y-chromosome containing sperm cells, selecting a desired subpopulation, ablating an undesired subpopulation, and collecting both the subpopulations of sperm cells including the desired subpopulation and the ablated undesired subpopulation together, wherein the collected population of sperm cells is used to fertilize an egg.

The present disclosure provides flow cytometry system. The flow cytometry system includes a flow cell, a fluidic pathway in fluid communication with the flow cell, and a probe in fluid communication with the fluidic pathway. The probe is configured to input a plurality of samples and aliquots of a separation gas between successive ones of the plurality of samples into the fluidic pathway. The flow cytometry system also includes two or more lasers positioned such that an illumination spot of each of the two or more lasers is directly on the flow cell, and two or more side scatter detection modules in communication with the two or more lasers. The flow cytometry system also includes a processor in communication with the two or more side scatter detection modules, and a non-transitory computer readable medium having stored therein instructions that are executable to cause the processor to perform functions when using the flow cytometer system. The functions include determining a time delta between a first laser of the two or more lasers and a second laser of the two or more lasers based on a difference between a plurality of first timestamps detected by a first side scatter detection module and a plurality of second timestamps detected by a second side scatter detection module.

The present invention relates to a method for developing and/or reprogramming plant cellular objects comprising the steps: providing a reservoir containing a medium with plant cellular objects: providing a first set of compartments of sample fluid embedded in carrier fluid in a microfluidic conduit, wherein the carrier fluid is immiscible with the medium, wherein the first set's compartments of sample fluid each comprise medium and at least one plant cellular object: providing one or more first state triggers to the plant cellular objects in the microfluidic conduit for inducing a first state in the plant cellular objects of the first set of compartments: incubating the plant cellular objects of the first set of compartments in the microfluidic conduit for a time span sufficient for the plant cellular objects to transfer to the first state: selecting one or more first selection parameters indicative of the first state: identifying, within the first set of compartments in the microfluidic conduit, compartments according to the one or more first selection parameters and optionally assigning the compartments with respective state identifiers.

System and method for handling dispersed microscopic objects contained in a sample fluid, the system comprising a first microfluidic device comprising a microchannel with an inlet, an outlet and an opening, the opening being located between the inlet and the outlet, a conveying device configured to pump a carrier fluid via the inlet into the microchannel with an input volumetric flow rate and to remove fluid from the microchannel via the outlet with an output volumetric flow rate, a first sensor unit for identifying positions of the dispersed objects in the sample fluid, and a positioning unit configured to position the opening at a target position proximate the position of a target object.