There is provided technology that enables a microparticle sorting device to be adjusted highly accurately without using adjustment beads. The present technology provides a microparticle sorting device including a light detection unit that optically detects a microparticle flowing through a flow path, a droplet forming unit that forms a droplet containing the microparticle, and a device adjustment unit that adjusts the device, in which, in a process of adjusting the device before actual measurement of the microparticle, the device adjustment unit performs optical axis position calibration for calibrating a relative position of the flow path relative to irradiation light and delay time calibration for calibrating a delay time from light irradiation to the microparticle to formation of the droplet on the basis of information obtained from the microparticle to be measured.

A first imaging unit obtains an image of at least one of a jet flow, droplets or satellite drops. Based on a feature value of the at least one of the jet flow, the droplets or the satellite drops in the image, a controller controls a timing of starting to supply charges from a charge supply unit to a final jet flow droplet in one period of vibrations of a vibration element or an amplitude of a drive voltage applied to the vibration element so as to cause variation of a side stream to fall within a reference range.

This disclosure concerns a cytometry system including a handling system that enables presentation of single cells to at least one laser source. The laser source is configured to deliver light to a cell within the cells in order to induce bond vibrations in the cellular DNA. The system further includes a detection facility that detects the signature of the bond vibrations, wherein the bond vibration signature is used to determine the folding or packing of the DNA.

This disclosure concerns a cytometry system including a handling system that enables presentation of single cells to at least one laser source. The laser source is configured to deliver light to a cell within the cells in order to induce bond vibrations in the cellular DNA. The system further includes a detection facility that detects the signature of the bond vibrations, wherein the bond vibration signature is used to determine the folding or packing of the DNA.

The apparati, methods, and computer program products disclosed herein can be used to nondestructively detect undissolved particles, such as glass flakes and/or protein aggregates, in a fluid in a vessel, such as, but not limited to, a fluid that contains a drug.

A system for orienting particles in a microfluidic system includes one or more radiation pressure sources arranged to expose particles to radiation pressure to cause the particles to adopt a particular orientation in the fluid. A system for sorting particles in a microfluidic system includes a detection stage arranged to detect at least one difference or discriminate between particles in the fluid flow past the detection stage, and one or more radiation pressure sources past which the particles move sequentially and a controller arranged to switch radiation energy to cause a change in direction of movement of selected particles in the fluid flow to sort the particles. The particles may be biological particles such as spermatazoa. The radiation pressure may be optical pressure and may be from one or more waveguides which may extend across a channel of the microfluidic system.

The apparati, methods, and computer program products disclosed herein can be used to nondestructively detect undissolved particles, such as glass flakes and/or protein aggregates, in a fluid in a vessel, such as, but not limited to, a fluid that contains a drug.

A measurement system includes a system for causing relative motion between a sample and an irradiation spot. The sample includes fluorescent markers having respective wavelengths. A gating system provides a gating signal based at least in part on resultant light substantially at an irradiation wavelength. A detection system detects fluorescent light from the irradiated markers and provides detection signals representing the fluorescent light detected concurrently with a gate-open signal. In some examples, the detection system detects fluorescent light at multiple wavelengths and provides respective detection signals. A spectral discriminator arranged optically between the sample and the detection system receives the fluorescent light from the sample and provides respective fluorescent light at the wavelengths to the detection system. A flow cytometer can spectrally disperse resultant fluorescent light and measure the wavelengths separately. Light from a sample disposed over a reflective phase grating can be dispersed, measured, and gated.

Presented is a device for detecting particles, comprising: a first light source positioned for illuminating particles passing through a detection region of the particle detector; a first detector positioned and adapted for detecting light signals from particles illuminated by the first light source in the detection region; a processor configured for determining a type of the particles passing through the detection region from light signals detected by the first detector; characterized in that: the particle detector further comprises: a means for detecting when particles pass through the detection region; and a controller coupled to the means and configured to operate the first light source with a first pulsed current when particles pass through the detection region thereby preserving or extending lifetime of the first light source, and wherein the first pulsed current is selected beyond a continuous current damage threshold of the first light source thereby increasing light output of the first light source.

Aspects of the present disclosure include methods and systems for detecting light from a sample in a flow stream by multi-photon counting. Methods according to certain embodiments include irradiating a sample in a flow stream with a light source and detecting light from the sample in the flow stream and counting photons of the detected light by integrating photo-electron charge over a time interval. Methods also include irradiating a sample in a flow stream with a light source, detecting light from the sample in the flow stream and outputting a digital output signal and an analog output signal produced by the detected light. Systems for detecting light from a sample in a flow stream with a detector and counting photons by integrating photo-electron charge over a time interval are also described. Kits having a detector, a photon counter and a flow cell configured to propagate a sample in flow stream are also provided.