A method and a system for fluorescence intensity of a fluorescence image are provided. In the method, fluorescence imaging is performed on a target sample to obtain a fluorescent image. Edge extraction and segmentation is performed on each detection target in the fluorescence image, to obtain the fluorescent image area of each detection target in the fluorescence image. At least one of a cumulative gray-scale value, a maximum gray-scale value and an average gray-scale value of the fluorescence image region and a diameter value of a bright field image region of each detected target is calculated. Then, the flow clustering analysis is calculated based on at least one of the cumulative grayscale value, the maximum grayscale value, the average grayscale value, and the bright field diameter value.

Disclosed is a cell analysis method comprising: extracting target cells from a population of cells derived from an epithelial tissue on the basis of N/C ratio representing a relative size of a nucleus to a cytoplasm; classifying the target cells into at least a first group and a second group by difference of amount of DNA; and evaluating a pathology of the epithelial tissue by comparing a ratio of numbers of cells between the first and second groups with a threshold; wherein the threshold varies according to a proportion of the target cells in the population.

In some embodiments, a plurality of smart flow cytometers are coupled into communication with a computer communication network. A central repair server system is coupled into communication with the computer communication network and the plurality of smart flow cytometers. Each of the plurality of smart flow cytometers includes a monitoring system coupled to monitor differing operational parameters of the smart flow cytometer for possible failure. The monitoring system can detect an advanced failure of components based on the operational parameters being monitored. The monitoring system can also detect an advanced need for repair and maintenance based on the operational parameters being monitored.

The present invention provides a portable air quality sensing device and a corresponding air quality sensing method. The portable air quality sensing device is equipped with environmental parameter detecting means (10) configured to detect local readings of one or more environmental parameters affecting air quality; retrieval means (150) for retrieving at least one global AQI value and/or at least one global measurement of an environmental parameter affecting air quality contributing to the global AQI value from at least one remote reading data source means (200) via a network (500); and an evaluation device (20) configured to form a local AQI value based on said one or more detected local readings and said at least one global AQI value and/or on said at least one retrieved global reading.

In some embodiments, a smart flow cytometer includes a monitoring system to monitor differing operational parameters of the smart flow cytometer to detect an advanced failure of components and an advanced need for maintenance. In others, a smart flow cytometer includes a quality control system including a reservoir of quality control beads to periodically run a validation test with the quality control beads in order to determine the ability of the flow cytometer to generate quality data output from a plurality of light detectors. In some embodiments, a plurality of smart flow cytometers are coupled into communication with a computer communication network; a central repair server system is coupled into communication with the computer communication network and the plurality of smart flow cytometers; wherein each of the plurality of smart flow cytometers includes a monitoring system coupled to monitor differing operational parameters of the smart flow cytometer for possible failure.

An optical particle sensor comprises at least first and second light sources of different wavelength for sequential operation. An optical detector is used to detect light from the light sources emitted or scattered by particles to be sensed. A current injection compensation signal is also provided which is dependent on which light source of the optical arrangement is in use. The compensation signal means the amplifier does not need to re-settle in response to different background illumination levels associated with the different light sources. In this way, detection signals may be obtained in quick succession from different light sources.

Systems for detecting light (e.g., in a flow stream) are described. Light detection systems according to embodiments include a photodetector, an input modulator configured to modulate signal input into the photodetector and an output modulator configured to modulate signal output from the photodetector. Photodetector arrays having a plurality of light detection systems, e.g., as described, are also provided. Methods for matching output signals from two or more photodetectors (e.g., a plurality of photomultiplier tubes in a photodetector array) are also described. Flow cytometer systems and methods for detecting light from a sample in a flow stream are provided. Aspects further include kits having two or more of the subject light detection systems.

Disclosed is an information processing method to be executed by a computer, for analysis performed by a flow cytometer capable of acquiring a test result through a test using an antibody reagent, the information processing method including: acquiring a first test result obtained through a first test performed by the flow cytometer with respect to a first specimen of a subject; acquiring a second test result obtained through a second test different from the first test with respect to a second specimen of the subject; and displaying the first test result and the second test result in association with each other.

A laser sensor for detecting a particle density includes: a laser configured to emit a measurement beam, an optical arrangement being arranged to focus the measurement beam to a measurement volume, the optical arrangement having a numerical aperture with respect to the measurement beam, a detector configured to determine a self-mixing interference signal of a optical wave within a laser cavity of the laser, and an evaluator. The evaluator is configured to: receive detection signals generated by the detector in reaction to the determined self-mixing interference signal, determine an average transition time of particles passing the measurement volume in a predetermined time period based on a duration of the self-mixing interference signals generated by the particles, determine a number of particles based on the self-mixing interference signals in the predetermined time period, and determine the particle density based on the average transition time and the number of particles.

Presented is a device (100) for detecting particles, comprising: a first light source (101) positioned for illuminating particles (120) passing through a detection region (130) of the particle detector; a first detector (104) positioned and adapted for detecting light signals from particles illuminated by the first light source (101) in the detection region (130); a processor (110) configured for determining a type of the particles passing through the detection region (130) from light signals detected by the first detector (104); characterized in that: the particle detector (100) further comprises: a means (105) for detecting when particles pass through the detection region (130); and a controller (103) coupled to the means (105) and configured to operate the first light source (101) with a first pulsed current when particles pass through the detection region (130) thereby preserving or extending lifetime of the first light source (101), and wherein the first pulsed current is selected beyond a continuous current damage threshold of the first light source (101) thereby increasing light output of the first light source (101).