An optical measuring apparatus and an operating method thereof are disclosed. The optical measuring apparatus includes a light source, a carrier chip, a light sensor, an analyzing chip and a display. Samples are uniformly distributed on the carrier chip. The light source emits sensing lights toward the carrier chip. The light sensor receives the sensing lights passing through the carrier chip at a plurality of times to obtain a plurality of images corresponding to the plurality of times respectively. The analyzing chip is coupled to the light sensor. The analyzing chip analyzes the object number and distribution variation with time in the sample according to the plurality of images corresponding to the plurality of times and estimates intrinsic characteristics of the object in the sample accordingly. The display is coupled to the analyzing chip. The display displays the intrinsic characteristics of the object in the sample.

A pretreatment apparatus includes a sample dispensing part 240, a reagent dispensing part 280 for dispensing a labeling reagent, a first process part 210 having a centrifuge device for performing a centrifugation process, a second process part 220, and a control part for distributing the dispensing destination of the sample to either the first sample container 217 or the second sample container 227 according to whether centrifugation process is required for the sample.

A system, an apparatus, and a method are provided for a modular flow cytometer with a compact size. In one embodiment, the modular flow cytometry system includes the following: a laser system for emitting laser beams; a flow cell assembly positioned to receive the laser beams at an interrogation region of a fluidics stream where fluoresced cells scatter the laser beams into fluorescent light; a fiber assembly positioned to collect the fluorescent light; and a grating system including a dispersive element and a receiver assembly, wherein the dispersive element is positioned to receive the fluorescent light from the fiber assembly and to direct spectrally dispersed light toward the receiver assembly.

A hyperspectral imaging flow cytometer can acquire high-resolution hyperspectral images of particles, such as biological cells, flowing through a microfluidic system. The hyperspectral imaging flow cytometer can provide detailed spatial maps of multiple emitting species, cell morphology information, and state of health. An optimized system can image about 20 cells per second. The hyperspectral imaging flow cytometer enables many thousands of cells to be characterized in a single session.

The disclosed flow cytometer includes a wavelength division multiplexer (WDM). The WDM includes an extended light source providing light that forms an object, a collimating optical element that captures light from the extended light source and projects a magnified image of the object as a first light beam, and a first focusing optical element configured to focus the first light beam to a size smaller than the object of the extended light source to a first semiconductor detector. The disclosed flow cytometer further includes a composite microscope objective to direct light emitted by a particle in a flow channel in a viewing zone of the composite microscope to the extended light source, a fluidic system and a peristaltic pump configured to supply liquid sheath and liquid sample to the flow channel, and a laser diode system to illuminate the particle in the flow channel.

The present embodiments provide an analog to digital converter, including a beam splitter, M photodetectors, M amplifier modules, and an encoder. Each output end of the beam splitter is corresponding to an input end of a photodetector, an output end of each photodetector is connected to an input end of an amplifier module, and an output end of each amplifier module is connected to an input end of the encoder. The beam splitter splits an inputted analog optical signal into M optical signals, outputs each optical signal to a corresponding photodetector to convert each optical signal into a current signal, inputs each current signal to a corresponding amplifier module to generate an output voltage, and outputs the output voltage to a corresponding input end of the encoder.

In order to simply and accurately acquire a three-dimensional image of a sample to observe the sample, a light-sheet microscope according to the present invention includes: a duct in which the sample can flow; a syringe pump for moving the sample in the duct; a cylindrical lens for causing planar illumination light along a plane intersecting the direction in which the sample is moved by the syringe pump to enter the duct; an objective lens that is disposed so as to face the radiation plane of the illumination light caused by the cylindrical lens to enter the duct and that collects fluorescence emitted from the sample moved by the syringe pump so as to pass through the radiation plane of the illumination light; and a camera for acquiring an image of the fluorescence from the sample collected by the objective lens.

Aspects of the present disclosure include a particle sorting module for sorting components of a sample, such as cells in a biological sample. Particle sorting modules according to certain embodiments include an enclosed housing having an aligner for coupling the housing with a particle sorting system, a flow cell nozzle positioned at the proximal end of the housing, a sample interrogation region in fluid communication with the orifice of the flow cell nozzle and a droplet deflector. A particle sorting system and methods for separating components of a sample as well as kits, including one or more particle sorting module, suitable for coupling with a particle sorting system and for practicing the subject methods are also provided.

In some aspects, the present disclosure provides a biochip detecting device that comprises a first shell (2) connect to a second shell (1), and a rotatable support frame (5) of biochip possessing a detecting zone that holds one or more biochips, and a detector (7).

In flow cytometry, particles (2) can be distinguished between populations (8) by combining n-dimensional parameter data, which may be derived from signal data from a particle, to mathematically achieve numerical results representative of an alteration (48). An alteration may include a rotational alteration, a scaled alteration, or perhaps even a translational alteration. Alterations may enhance separation of data points which may provide real-time classification (49) of signal data corresponding to individual particles into one of at least two populations.