Provided are systems and methods that allow for brightfield imaging in a flow cytometer, allowing for collection of fluorescence and high-quality image date. The disclosed technology also gives rise to an illumination pattern that allows a user to create different oblique or structured illumination profiles within a static system. With the disclosed approach, a user can illuminate a sample from a first direction (e.g., with laser illumination configured to give rise to one or more of fluorescence information and scattering information), collect scattering information from a second direction, collect fluorescence information from a third direction, and capture an image of the sample from a fourth direction. (Two or more of the foregoing can be accomplished simultaneously.) Also as described elsewhere herein, an illumination used to illuminate the sample for visual image capture can be communicated to the same through a lens that also collects fluorescence from the sample.

Systems and methods for flow cytometry. The methods comprise: labeling cells of a sample with Raman tags; causing the sample to flow through a microfluidic channel of a flow cytometer through which a laser beam passes; detecting Raman signals emitted from the Raman tags while being illuminated by the laser beam; and determining characteristics of the cells based on the detected Raman signals.

An imaging system for multiplex detection of biomolecules, including at least a first optical path and a second optical path; the first optical path is from an excitation light source to an objective lens, and a filter set is provided between the excitation light source and the objective lens and is configured to guide excitation light from the excitation light source to the objective lens; the second optical path is from the objective lens to an image acquisition device, a reflector is provided between the objective lens and the image acquisition device and is configured to project the fluorescence received by the objective lens to the image acquisition device; the excitation light source includes at least a first excitation light source used for excitation of fluorescent signals and a second excitation light source used for excitation of light-initiated chemiluminescence signals The system simultaneously realizes fluorescence and light-initiated chemiluminescence imaging.

Metal oxide gel particles, may be prepared with a desired particle size, by preparing a low-temperature aqueous metal nitrate solution containing hexamethylene tetramine as a feed solution; and causing the feed solution to flow through a first tube and exit the first tube as a first stream at a first flow rate, so as to contact a high-temperature nonaqueous drive fluid. The drive fluid flows through a second tube at a second flow rate. Shear between the first stream and the drive fluid breaks the first stream into particles of the metal nitrate solution, and decomposition of hexamethylene tetramine converts metal nitrate solution particles into metal oxide gel particles. A metal oxide gel particle size is measured optically, using a sensor device directed at a flow of metal oxide gel particles within the stream of drive fluid. The sensor device measures transmission of light absorbed by either the metal oxide gel particles or the drive fluid, so that transmission of light through the drive fluid changes for a period of time as a metal oxide gel particle passes the optical sensor. If a measured particle size is not about equal to a desired particle size, the particle size may be corrected by adjusting a ratio of the first flow rate to a total flow rate, where the total flow rate is the sum of the first and second flow rates.

The present application discloses a nanoparticle recognition device and method based on detection of scattered light with electric dipole rotation. According to the scattering model of nanoparticles, the in situ detection of particle morphology in an optical trap is realized by the methods of particle suspension control and scattered light detection and separation. Specifically, two linearly polarized laser beams are used, wherein the first laser beam suspends nanoparticles and rotates nanoparticles by adjusting the polarization direction; the polarization direction of the second linearly polarized light is unchanged, and scattered light in a specific dipole direction is excited; the change of the polarizability of the nanoparticles is deduced by monitoring the change of the light intensity of the scattered light excited by the second laser beam at the fixed position, so that particle morphology recognition is realized.

Provided are a fringe information measuring apparatus that measures information on a fringe region using a temperature sensor array and a substrate treating system including the same. The fringe information measuring apparatus comprises: a laser sensor configured to output a first laser light and a second laser light to intersect each other; a thermal sensor array configured to pass through a fringe region formed by the intersection of the first laser light and the second laser light; and a control module configured to measure a position of the fringe region based on information obtained when the thermal sensor array passes through the fringe region.

Described herein are apparatuses for analyzing an optical signal decay. In some embodiments, an apparatus includes: a source of a beam of pulsed optical energy; a sample holder configured to expose a sample to the beam; a detector comprising a number of spectral detection channels configured to convert the optical signals into respective electrical signals; and a signal processing module configured to perform a method. In some embodiments, the method includes: receiving the electrical signals from the detector; mathematically combining individual decay curves in the electrical signals into a decay supercurve, the supercurve comprising a number of components, each component having a time constant and a relative contribution to the supercurve; and numerically fitting a model to the supercurve.

An optical proximity sensor includes a first vertical cavity surface-emitting laser configured for self-mixing interferometry to determine distance to and/or velocity of an object. The optical proximity sensor also includes a second vertical cavity surface-emitting laser configured for self-mixing interferometry to determine whether any variation in a fixed distance has occurred. The optical proximity sensor leverages output from the second vertical cavity surface-emitting laser to calibrate output from the second vertical cavity surface-emitting laser to eliminate and/or mitigate environmental effects, such as temperature changes.

The present relates to a measurement device (1) for the detection and/or measurement of particles in a fluid, the measurement device comprising a fluid source (11) for producing a flow of fluid (111) along a fluid flow path, a first laser source (12) positioned for emitting a first laser beam (120) of laser light in a measurement volume of the fluid flow path for light scattering; a scattered light detecting means (13) for detecting a presence of a particle in the fluid flow path through detection and measurement of laser beam light scattered on different angles by said particle, wherein it further comprises a second laser source (14) positioned for emitting a second laser beam (140) of laser light in said measurement volume of the fluid flow path for Raman and fluorescence excitation; a Raman and fluorescence detecting means (15) for detecting a Raman scattering signal emitted by the fluid and a Fluorescence signal emitted by said particle upon excitation by said second laser beam (140).

Described herein are apparatuses and methods for analyzing an optical signal decay. In some embodiments, an apparatus includes: a source of a beam of pulsed optical energy; a sample holder configured to expose a sample to the beam; a detector comprising a number of spectral detection channels configured to convert the optical signals into respective electrical signals; and a signal processing module configured to perform a method. In some embodiments, the method includes: receiving the electrical signals from the detector; mathematically combining individual decay curves in the electrical signals into a supercurve, the supercurve comprising a number of components, each component having a time constant and a relative contribution to the supercurve; and quantifying a relative contribution of each component to the supercurve.