In one aspect, a system for performing flow cytometry is disclosed, which comprises a laser for generating laser radiation for illuminating a sample, at least one detector for detecting at least a portion of a radiation emanating from the sample in response to said illumination so as to generate a temporal signal corresponding to said detected radiation, and an analysis module for receiving said temporal signal and performing a statistical analysis of said signal based on a forward model to reconstruct an image of said sample.

Fluorescence imaging system designs are described that provide larger fields-of-view, increased spatial resolution, improved modulation transfer and image quality, higher spatial sampling frequency, faster transitions between image capture when repositioning the sample plane to capture a series of images (e.g., of different fields-of-view), and improved imaging system duty cycle, and thus enable higher throughput image acquisition and analysis for genomics and other imaging applications.

A device (1) is described and represented for the determination of the particle size and/or the particle shape and/or optical properties, such as transparency, of particles (2) in a particle stream (3), with a feeding device (4) for the feeding of the particles (2) to a measuring zone (5), wherein the particles (2) flow through the measuring zone (5), with at least one illuminating device (6) for illuminating the measuring zone (5), with at least two camera devices (7, 8), each of which photographs a measurement region (9, 10) of the measuring zone (5) associated with the corresponding camera device (7, 8), wherein a first camera device (7) photographs a first, preferably larger, measurement region (10) with a first, preferably lesser, magnification and a second camera device (8) photographs a second, preferably smaller, measurement region (9) with a second, preferably stronger, magnification, with an imaging optics (11) for imaging the measurement regions (9, 10), and with an evaluating device for determining the particle size and/or the particle shape from the photographs of the measurement regions (9, 10), wherein the imaging optics (11) comprises at least one optical element (14), at which and/or by which the light radiation emanating from the measuring zone (5) is divided into at least two beam portions. According to the invention, it is provided that the illuminating device (6) is designed such that the first measurement region (10) and the second measurement region (9) are always illuminated together, wherein the first measurement region (10) is illuminated with the same intensity as the second measurement region (9).

A device for optically detecting in transmission nanoparticles moving in a fluid sample includes a light source for emitting a spatially incoherent beam for illuminating the sample; an imaging optical system; and a two-dimensional optical detector. The imaging optical system includes a microscope objective. The two-dimensional optical detector includes a detection plane conjugated with an object focal plane of the microscope objective by said imaging optical system. The two-dimensional optical detector allows a sequence of images of an analysis volume of the sample to be acquired, each image resulting from optical interferences between the illuminating beam incident on the sample and the beams scattered by each of the nanoparticles present in the analysis volume during a preset duration shorter than one millisecond. The device further includes an image processor that allows an average of a sequence of said images to be taken and said average to be subtracted from each image in order to determine, for each nanoparticle of the analysis volume, the amplitude of the scattered beam.

A hyperspectral imaging flow cytometer can acquire high-resolution hyperspectral images of particles, such as biological cells, flowing through a microfluidic system. The hyperspectral imaging flow cytometer can provide detailed spatial maps of multiple emitting species, cell morphology information, and state of health. An optimized system can image about 20 cells per second. The hyperspectral imaging flow cytometer enables many thousands of cells to be characterized in a single session.

Systems and methods in accordance with various embodiments of the invention are capable of rapid analysis and classification of cellular samples based on cytomorphological properties. In several embodiments, cells suspended in a fluid medium are passed through a microfluidic channel, where they are focused to a single stream line and imaged continuously. In a number of embodiments, the microfluidic channel establishes flow that enables individual cells to each be imaged at multiple angles in a short amount of time. A pattern recognition system can analyze the data captured from high-speed images of cells flowing through this system and classify target cells. In this way, the automated platform creates new possibilities for a wide range of research and clinical applications such as (but not limited to) point of care services.

The disclosed flow cytometer includes a wavelength division multiplexer (WDM). The WDM includes an extended light source providing light that forms an object, a collimating optical element that captures light from the extended light source and projects a magnified image of the object as a first light beam, and a first focusing optical element configured to focus the first light beam to a size smaller than the object of the extended light source to a first semiconductor detector. The disclosed flow cytometer further includes a composite microscope objective to direct light emitted by a particle in a flow channel in a viewing zone of the composite microscope to the extended light source, a fluidic system and a peristaltic pump configured to supply liquid sheath and liquid sample to the flow channel, and a laser diode system to illuminate the particle in the flow channel.

In order to simply and accurately acquire a three-dimensional image of a sample to observe the sample, a light-sheet microscope according to the present invention includes: a duct in which the sample can flow; a syringe pump for moving the sample in the duct; a cylindrical lens for causing planar illumination light along a plane intersecting the direction in which the sample is moved by the syringe pump to enter the duct; an objective lens that is disposed so as to face the radiation plane of the illumination light caused by the cylindrical lens to enter the duct and that collects fluorescence emitted from the sample moved by the syringe pump so as to pass through the radiation plane of the illumination light; and a camera for acquiring an image of the fluorescence from the sample collected by the objective lens.

Systems for collecting light (e.g., in a flow stream) are described. Light collection systems according to embodiments include: a mount having an orifice for receiving light, an adapter configured for attaching a camera to the mount and a fastener for attaching a lens to the distal end of the mount and a releasably attachable connecter that is configured for coupling to an orifice plate and an aligner that is configured to couple with an aligner on the mount and maintain optical alignment between the mount and connector. Methods for coupling a connector and a mount are also described. Systems and methods for measuring light emitted by a sample (e.g., in a flow stream) are also provided.

A cell sorting system is provided to comprise: an imaging device including abeam scanner scanning abeam along a first direction to obtain a cell image data including fluorescent information or cell image information of a cell, the beam applied to the cell flowing in a channel along a second direction with an angle to the first direction; a data processing and control device in communication with the imaging device, the data processing and control device including a processor configured to process the cell image data obtained by the imaging device to determine one or more properties associated with the cell from the processed cell image data and to produce a control command based on a comparison of the determined one or more properties with a sorting criteria, and a cell sorting device in communication with the imaging device and the data processing and control device.