A measurement apparatus according to an embodiment of the present technology includes a light source, a filling portion, and a detector. The light source emits illumination light. The filling portion includes a first surface portion and a second surface portion which are provided on an optical path of the illumination light and are opposite to each other, the filling portion enabling a cavity between the first and second surface portions to be filled with liquid including a cell. The detector detects an interference fringe of the illumination light passing through the cavity, the interference fringe being caused by the liquid including the cell.

In one aspect, the present teachings provide a system for performing cytometry that can be operated in three operational modes. In one operational mode, a fluorescence image of a sample is obtained by exciting one or more fluorophore(s) present in the sample by an excitation beam formed as a superposition of a top-hat-shaped beam with a plurality of beams that are radiofrequency shifted relative to one another. In another operational mode, a sample can be illuminated successively over a time interval by a laser beam at a plurality of excitation frequencies in a scanning fashion. The fluorescence emission from the sample can be detected and analyzed, e.g., to generate a fluorescence image of the sample. In yet another operational mode, the system can be operated to illuminate a plurality of locations of a sample concurrently by a single excitation frequency, which can be generated, e.g., by shifting the central frequency of a laser beam by a radiofrequency. For example, a horizontal extent of the sample can be illuminated by a laser beam at a single excitation frequency. The detected fluorescence radiation can be used to analyze the fluorescence content of the sample, e.g., a cell/particle.

A laser sensor module for detecting a particle density of small particles with a particle size between 0.05 μm and 10 μm includes a first laser configured to emit a first measurement beam, a second laser configured to emit a second measurement beam, and an optical arrangement configured to focus the first measurement beam to a first measurement volume and to focus the second measurement beam to a second measurement volume. The optical arrangement includes a first numerical aperture and a second numerical aperture arranged to detect a predetermined minimum particle size. The laser sensor module further includes a first detector configured to determine a first self-mixing interference signal of a first optical wave, a second detector configured to determine a second self-mixing interference signal of a second optical wave, and an evaluator.

Holographic Video Microscopy analysis of non-spherical particles is disclosed herein. Properties of the particles are determined by application of light scattering theory to holography data. Effective sphere theory is applied to provide information regarding the reflective index of a sphere that includes a target particle. Known particles may be co-dispersed with unknown particles in a medium and the holographic video microscopy is used to determine properties, such as porosity, of the unknown particles.

A device (100) for visualization of one or more components in a blood sample is disclosed. In one aspect, the device (100) includes an imaging module (110), wherein the imaging module (110) includes a controllable illumination source (102) capable of emitting light in plurality of discrete angles; a tube lens (105); one or more objective lens (104); and an image capturing module (106). Additionally, the device (100) includes a channel (103) configured to carry the blood sample, wherein the channel (103) is capable of sorting the one or more components in the blood sample.

A particle analysis method and apparatus, including a spectrometry-based analysis of a fluid sample (1), comprises the steps of creating a sample light beam S and a probe light beam P with a light source device (10) and periodically varying a relative phase between the sample and probe light beams S, P with a phase modulator device (20), irradiating the fluid sample (1) with the sample light beam S, detecting the sample and probe light beams S, P with a detector device (40), and providing a spectral response of the at least one particle (3), wherein the light source device (10) comprises at least one broadband source, which has an emission spectrum covering a mid-infrared MIR frequency range, and the phase modulator device (20) varies the relative phase with a scanning period equal to or below the irradiation period of irradiating the at least one particle (3, 4).

To optimize an imaging range in a depth direction in terms of a relationship with a resolution, an OCT apparatus includes a signal processor that determines a reflected light intensity distribution of an imaging object on the basis of a spectrum of a detected interference light. The signal processor performs spectrum conversion, having a conversion characteristic with which a light source spectrum is converted to a Gaussian distribution curve, on the spectrum of the interference light, and determines the reflected light intensity distribution by Fourier-transforming a spectrum resulting from the spectrum conversion. In the conversion characteristic, the light source spectrum and the Gaussian distribution curve have center wavelengths differing from each other.

A device for detecting (D) at least one predetermined particle (P) includes an interferometric element (EI) arranged so as to be illuminated by an incident radiation (L.sub.in) and comprising at least one so-called thin layer (CM) disposed on top of a so-called substrate layer (Sub), the particle being attached to a surface (Sm) of the thin layer, the interferometric element (EI) forming a Fabry-Pérot cavity with or without attached particle P; a matrix sensor (Det) adapted to detect an image comprising a first portion (P.sub.1) deriving from the detection of the incident radiation transmitted (L.sub.TBG) by the interferometric element alone and a second portion (P.sub.2) deriving from the detection of the incident radiation transmitted (L.sub.TP) by the interferometric element and any particle (O, P) attached to a surface (Sm) of the thin layer; a processor (UT) linked to the sensor and configured: to calculate, as a function of wavelengths of the incident radiation λ.sub.i i∈[1,m], the variation of intensity of at least one first pixel of the first portion, called first variation (F.sub.BG) and of at least one second pixel of the second portion, called second variation (F.sub.P), to determine a trend, as a function of the wavelengths of the incident radiation λ.sub.i i∈[1,m], of a phase shift ϕ.sub.i between the first variation and the second variation; to detect the attached particle when the phase shift ϕ.sub.i is not constant as a function of the wavelengths of the incident radiation λ.sub.i i∈[1,m].

An optical measurement system comprises a polarization beam splitter for dividing an incident beam into a reference beam and a measurement beam, a first beam splitter for reflecting the measurement beam to form a first reflected measurement beam, a spatial light modulator for modulating the first reflected measurement beam to form a modulated measurement beam, a condenser lens for focusing the modulated measurement beam to an object to form a penetrating measurement beam, an objective lens for converting the penetrating measurement beam into a parallel measurement beam, a mirror for reflecting the parallel measurement beam to form an object beam, a second beam splitter for reflecting the reference beam to a path coincident with that of the object beam, and a camera for receiving an interference signal generated by the reference beam and the object beam to generate an image of the object.

Various embodiments are directed to a device for detecting fluid particle characteristics comprising: a collection fluid dispense assembly configured to selectively dispense a volume of collection fluid onto an absorbent media disposed within an internal sensor portion of a fluid composition sensor, producing a collection media based on interaction between the volume of collection fluid and the absorbent media; and a controller configured to determine, based on a particle image captured by an imaging device, a particle characteristic associated with a particle captured at the collection media. In various embodiments a device is configured to receive therein a collection media comprising a biologically nutritive substance; and may comprise an imaging device and a controller configured to determine a biological particle characteristic based on a comparison of first particle data and second particle data generated by the imaging device, the second particle data being associated with an incubated particle configuration.