A system for deforming and analyzing a plurality of particles carried in a sample volume includes a substrate defining an inlet, configured to receive the sample volume, and an outlet; and a fluidic pathway fluidly coupled to the inlet and the outlet. The fluidic pathway includes a delivery region configured to receive the plurality of particles from the inlet and focus the plurality of particles from a random distribution to a focused state, a deformation region defining an intersection located downstream of the delivery region and coupled to the outlet, and wherein the deformation region is configured to receive the plurality of particles from the delivery region and to transmit each particle in the plurality of particles into the intersection from a single direction, a first branch fluidly coupled to the deformation region and configured to transmit a first flow into the intersection, and a second branch fluidly coupled to the deformation region and configured to transmit a second flow, substantially opposing the first flow, into the intersection, wherein the first flow and the second flow are configured to induce extension of one or more particles in the plurality of particles.

A system and method for determining a trajectory parameter of particles, comprising receiving a plurality of particles at a microfluidic channel, applying a force to each particle of the microfluidic channel, acquiring a dataset of each particle, measuring a trajectory of the particle, and determining a trajectory parameter of the particles.

The present invention is to facilitate analysis of a plurality of analysis items. A cell analysis method for analyzing cells is provided in which data for analysis of cells contained in a sample are generated, and an artificial intelligence algorithm to be the input destination of the generated analysis data is selected from among a plurality of artificial intelligence algorithms, data indicating the properties of the cells are generated based on the analysis data via the artificial intelligence algorithm.

The present invention relates to methods and systems for image cytometry analysis, typically at low optical magnification, where analysis is based on detection of biological particles using UV bright field and optionally one or more sources of excitation light.

Methods and systems for evaluating fertility potential of a living sperm cell are presented and involve providing a predetermined classification function correlating between one or more physio spatial parameters of sperm cells and DNA fragmentation distribution in sperm cells, providing data indicative of the one or more physio spatial parameters of the living sperm cell, and applying the classification function to the one or more physio spatial parameters of the living sperm cell to thereby determine a DNA fragmentation level, inside the DNA fragmentation distribution, in the living sperm cell, the DNA fragmentation level being indicative of the fertility potential of the living sperm cell.

The present disclosure provides devices, kits, and methods for enriching/separating and/or subtyping target cells from a biological sample, such as circulating tumor cells or other types of rare cells or differentiating cells. Devices, kits, and methods of the present disclosure utilize a created chemogradient to modulate movement of target and/or non-target cells in a sample based on attraction and/or repulsion to certain chemical compounds to separate and subtype cells in a sample.

A system and method for determining a trajectory parameter of particles, comprising receiving a plurality of particles at a microfluidic channel, applying a force to each particle of the microfluidic channel, acquiring a dataset of each particle, measuring a trajectory of the particle, and determining a trajectory parameter of the particles.

In some examples, a system including a fluid stream monitoring system. The monitoring system includes an illumination device configured to illuminate at least some of particles suspended in a fluid stream; and an imaging device configured to image the illuminated particles at a first image plane that intersects a longitudinal axis of the fluid stream, wherein the illumination device and the imaging device are registered to the fluid stream delivery device in the first image plane, where the first image plane is substantially orthogonal to the longitudinal axis. The system includes processing circuitry configured to determine one or more physical characteristics of the fluid particles.

Provided herein are systems and method for measuring cell stiffness. In particular, provided herein are microelectrode configuration and systems for measuring platelet deformation and stiffness.

Aspects of the present disclosure include a method for sorting cells of a sample based on an image of a cell in a flow stream. Methods according to certain embodiments include detecting light from a sample having cells in a flow stream, generating an image mask of a cell from the sample and sorting the cell based on the generated image mask. Systems having a processor with memory operably coupled to the processor having instructions stored thereon, which when executed by the processor, cause the processor to generate an image mask of a cell in a sample in a flow stream and to sort the cell based on the generated image mask are also described. Integrated circuit devices (e.g., field programmable gate arrays) having programming for generating an image mask and for determining one or more features of the cell are also provided.