The present invention provides a fluorescence detection chip, a fluorescence detection system, a fluorescence detection method and an application thereof. The fluorescence detection chip comprises an upper casing and a lower casing, and further comprises a sample cell, a mixed liquid cell, a waste liquid cell and a number of independent reaction detection cells, wherein the reaction detection polls have cell walls made of a superconducting thermal material; wherein the sample cell, the mixed liquid cell, the waste liquid cell and the reaction detection cells are all arranged in the lower casing, and in the upper casing a number of feeding ports are provided, which correspond to the sample cell, the mixed liquid cell and the reaction detection cells, respectively. By means of a micro-channel design, a reaction liquid is fully mixed during the flow process, and is evenly distributed to the respective reaction detection cells.

An apparatus and method is disclosed for rapid identification of a microorganism within sampling device. The sampling device has a plurality of reaction chambers each having a different reactive agent for reacting with the microorganism to indicate the presence of a microorganism in said reaction chamber. A detector detects each of the plurality of reaction chamber for detecting the presence of a microorganism in said reaction chamber. The invention automates CRISPR CAS 12 and/or CAS 13 method. The invention is a general platform for detection of segments of DNA or RNA using CRISPR CAS 12 and/or CAS 13 proteins.

A sensing device includes a sample loading chamber configured to receive a sample, a detection antibody drying or lyophilization chamber configured to receive a first portion of the sample, one or more substrate drying or lyophilization chambers configured to receive a second portion of the sample, and one or more reaction chambers connected to the detection antibody drying or lyophilization chamber and the one or more substrate drying or lyophilization chambers. The detection antibody drying or lyophilization chamber and one or more substrate drying or lyophilization chambers are placed in parallel between the sample loading chamber and the one or more reaction chambers

This present invention relates generally to devices, systems, and methods for performing optical and electrochemical assays and, more particularly, to devices and systems having universal channel circuitry configured to perform optical and electrochemical assays, and methods of performing the optical and electrochemical assays using the universal channel circuitry. The universal channel circuitry is circuitry that has electronic switching capabilities such that any contact pin, and thus any sensor contact pad in a testing device, can be connected to one or more channels capable of taking on one or more measurement modes or configurations (e.g., an amperometric measurement mode or a current drive mode).

A microfluidic device for evaluation of an organic/inorganic scale inhibitor is provided. The device comprises a substrate mountable to a disc for rotation about an axis. The device further comprises a proximal end and a distal end. The substrate defines a sample reservoir, a solvent reservoir, an inhibitor reservoir, and a precipitant reservoir at the proximal end and an analysis chamber at the distal end in fluid communication with the sample, solvent, inhibitor, and precipitant reservoirs. The substrate is constructed to direct one or more of fluids in the sample reservoir, solvent reservoir, inhibitor reservoir, and precipitant reservoir radially outwardly towards the analysis chamber under the influence of centrifugal force when the microfluidic device rotates.

An apparatus includes a housing and an actuator. The housing, which defines a reagent volume that can receive a reagent container, can be removably coupled to a reaction chamber. The housing includes a puncturer that defines a transfer pathway in fluid communication with the reagent volume. A delivery portion of the housing defines a delivery pathway between the transfer pathway and the reaction chamber when the housing is coupled to the reaction chamber. The actuator has a plunger portion disposed within the reagent volume. An engagement portion of the actuator can be manipulated to move the plunger portion within the reagent volume to deform the reagent container. The puncturer can pierce a frangible portion of the reagent container to convey a reagent from the reagent container into the reaction chamber via the transfer pathway and/or the delivery pathway.

The present invention provides self-contained systems for performing an assay for determining a chemical state, the system including a stationary cartridge for performing the assay therein, at least one reagent adapted to react with a sample; and at least one reporter functionality adapted to report a reaction of the at least one reagent with said sample to report a result of the assay, wherein the at least one reagent, the sample and the at least one reporter functionality are contained within the cartridge.

In some aspects, reader systems for optically detecting binding agents or analyte complexes in a sample as a result of performing biochemical assays can include: a housing defining a positioning receptacle to receive the sample; an excitation source to generate incident light directed at the sample; at least one solid-state photomultiplier detector configured to: i) receive a light emitted by at least one label associated with the binding agents and/or analyte complexes within the sample; and ii) produce a signal in response to receiving the light emitted by the at least one label or substrate solution that is physically or chemically modified by the said label, the at least one detector being connected to integrated signal processing electronics to process the signal; and a user interface in communication with the signal processing electronics for conveying one or more results of the one or more biochemical as says.

A method of evaluating an asphaltene inhibitor includes providing a centrifugal microfluidic system including: a disc mounted to rotate about an axis; a microfluidic device mounted on the disc, the device having sample, solvent, inhibitor, and precipitant reservoirs and an analysis chamber in fluid communication with the sample, solvent, inhibitor, and precipitant reservoirs; and an optical detection system coupled to the analysis chamber and configured to measure the optical transmission of fluid in the analysis chamber. The method includes filling the sample, solvent, inhibitor, and precipitant reservoirs, respectively, with a sample, solvent, inhibitor, and precipitant; rotating the disc to generate centrifugal force to cause the sample, solvent, inhibitor, and precipitant to travel radially outward to the analysis chamber; and measuring the optical transmission of a mixture of the sample, solvent, inhibitor, and precipitant in the analysis chamber as a function of radial distance of the analysis chamber.

The present invention relates to the field of biological sample testing technology, and in particular, to a biological sample reaction vessel. A reagent storage portion and a push rod movable relative to the reagent storage portion are packaged in the reaction vessel; the reagent storage portion comprises at least one reagent containing cavity, and the reagent containing cavity is sealed by a sealing element; and the push rod is connected to the sealing element, and the push rod is used for cooperation with an external device to separate the sealing element from the reagent storage portion. In this application, the reagent storage portion and the push rod are both packaged in the biological sample reaction vessel, and in reaction, the biological sample reaction vessel only needs to cooperate with a test cassette. With one operation, that is, inserting the biological sample reaction vessel into the external device, the reagent in the reagent storage portion can be released rapidly.