Provided are microfluidic systems and methods for detecting, sorting, and dispensing of low abundance events such as single cells and particles, including a variety of eukaryotic and bacterial cells, for a variety of bioassay applications. The systems and methods described herein, when implemented in whole or in part, will make relevant microfluidic based tools available for a variety of applications in biotechnology including antibody discovery, immuno-therapeutic discovery, high-throughput single cell analysis, target-specific compound screening, and synthetic biology screening.

Among other thing, the present invention provides solution to the problem, particularly, the present invention certain surfaces and certain sample holder to improve the sensitivity, speed, and easy-to-use of an optical signal based assays, such as colorimetric assays or fluorescence assays.

Provided are microfluidic systems and methods for detecting, sorting, and dispensing of low abundance events such as single cells and particles, including a variety of eukaryotic and bacterial cells, for a variety of bioassay applications. The systems and methods described herein, when implemented in whole or in part, will make relevant microfluidic based tools available for a variety of applications in biotechnology including antibody discovery, immuno-therapeutic discovery, high-throughput single cell analysis, target-specific compound screening, and synthetic biology screening.

Systems and methods are provided for characterizing a ply of fiber having a thermoplastic veil. One method includes acquiring an image of a fiber material comprising strands of fiber and further comprising a veil of filaments of thermoplastic, subdividing the image into slices, determining an amount of melted filaments depicted within each of the slices, and determining an amount of unmelted filaments depicted within each of the slices.

A portable colorimetric assay system includes an opto-mechanical reader configured to be detachably mounted to a mobile phone having a camera or other camera-containing portable electronic device. The opto-mechanical reader includes one or more light sources configured to illuminate a test sample holder and control sample holder disposed in the opto-mechanical reader along an optical path aligned with a camera of the mobile phone or other camera-containing portable electronic device. One or more serum separation membranes are disposed in the opto-mechanical reader and define a sample receiving pad configured to receive a blood sample. A moveable serum collection membrane is membrane is also disposed in the reader and is configured to contact the sample receiving pad in a first position and moveable to a second position where the serum collection membrane is disposed inside the test sample holder.

Provided are microfluidic systems and methods for detecting, sorting, and dispensing of low abundance events such as single cells and particles, including a variety of eukaryotic and bacterial cells, for a variety of bioassay applications. The systems and methods described herein, when implemented in whole or in part, will make relevant microfluidic based tools available for a variety of applications in biotechnology including antibody discovery, immuno-therapeutic discovery, high-throughput single cell analysis, target-specific compound screening, and synthetic biology screening.

The invention provides a mild procedure for the functionalization of cellulose and other substrates with a detection reagent such as N-(1-naphthyl)ethylenediamine and is able to achieve much higher functionalization density than previously reported. A paper-based device created using cellulose functionalized according to the invention allowed for much lower detection limits for nitrite in various kinds of water samples than have been seen using paper-based devices. In addition, grafting of N-(1-naphthyl)ethylenediamine to cellulose improved the stability of the N-(1-naphthyl)ethylenediamine in the presence of moisture and light.

A method for determining a property of a geological formation based on an optical image of rock samples taken from the formation is presented therein. The image comprises a plurality of pixels and the method comprises defining windows in the image, each window comprising predetermined number of pixels and being of a predetermined shape. The method also includes, for each window, extracting a rockprint value representative of the window. A rockprint comprises indicators for characterizing a texture of the window. The method also includes classifying the windows into categories of a predetermined set. Each category is representative of one type of rock and the classification is based on a comparison of the rockprint value of each window with rockprint values of images of reference rock samples for each category. Based on the classification, the method then includes determining the at least one property of the geological formation, ie the quantification of each type of rock in the sample.

A method of obtaining a plurality of infrared images of a banknote that involves simultaneously illuminating the banknote with infrared light at a first wavelength and infrared light at a second wavelength, capturing an image of the banknote with an RGB camera, obtaining from both a first output channel signal and a second output channel signal of the RGB camera sensor where the intensity distribution of the infrared light at the first wavelength and the intensity distribution of the infrared light at the second wavelength uses a first calibration coefficient and a second calibration coefficient of the RGB camera sensor, producing separate infrared images of the banknote at the first wavelength and the second wavelength from the respective intensity distributions.

Systems and methods for medical imaging and spectroscopy analysis are disclosed. Some embodiments relate to digital staining. Some embodiments relate to digital staining using hyperspectral or multispectral imaging. Some embodiments relate to digital staining using RGB imaging. Some embodiments relate to analysis of other types of medical imaging and spectroscopy. Some embodiments relate to platform for performing analysis of medical imaging and spectroscopy data.