Portable common path shearing interferometry-based holographic microscopy systems. The system includes a light source, a sample holder, a microscope objective lens, a shear plate and an imaging device positioned in a common path shearing interferometry configuration. A housing is configured to receive and hold the shear plate and maintain a position of the shear plate relative to the microscope objective lens.

Object interference in biological samples generated by lateral shearing interference microscopes is addressed by a shearing microscope slide comprising a periodic structure having alternating reference and sample regions. In some embodiments, the reference regions are configured to provide references that remove sample overlap in a sheared microscopic measurement. A system for generating sheared microscopic measurements is also provided that comprises an inlet configured to receive a sample material, an outlet configured to release a portion of the sample material, and a periodic structure having a plurality of interleaved reference and sample channels. In some cases, the sample channels are configured to accommodate a flow of sample material from the inlet to the outlet and the reference channels are configured to provide references that remove sample overlap in a sheared microscopic measurement.

In a microscopic observation unit (10), hologram data is acquired at each measurement position on a cell culture plate (13) while a light-source section (11) and other related sections are gradually moved by a moving section (15). Every time a set of data for one measurement position is acquired, a measurement monitoring image creator (24) creates a thumbnail image by reducing the size of a hologram image which is based on original data (two-dimensional distribution of light intensity). A display processor (25) pastes the create thumbnail image to progressively complete the hologram image of the entire plate to be displayed on a display unit (27). A measurement operator watches the hologram image during the execution of the measurement. When it has been concluded that the ongoing measurement is inappropriate, the operator presses a measurement stop button to immediately discontinue the measurement. Thus, When there is a problem with the measurement, such as a foreign object mixed in the sample, the measurement can be discontinued before a phase image or intensity image based on the hologram data is reconstructed on the server after the completion of the entire measurement, so as to avoid wasting time for the useless measurement.

The present invention relates to a novel label-free tomographic interferometry technique implemented inside an environmental chamber (e.g. imaging incubator) that provides rapid capturing of the three-dimensional (3-D) refractive index distribution of biological cells.

A determination method of non-destructively and easily determining a state of an aggregate of a plurality of cells formed by three-dimensional culture is provided. A determination method according to the disclosed technology includes generating a phase difference image of an aggregate of a plurality of cells from a hologram obtained by imaging the aggregate, deriving a first index value that indicates a randomness of an array of a phase difference amount in a plurality of pixels constituting the phase difference image, and determining a state of the cells constituting the aggregate on the basis of the first index value.

A device for detecting (D) at least one predetermined particle (P) includes an interferometric element (EI) arranged so as to be illuminated by an incident radiation (L.sub.in) and comprising at least one so-called thin layer (CM) disposed on top of a so-called substrate layer (Sub), the particle being attached to a surface (Sm) of the thin layer, the interferometric element (EI) forming a Fabry-Pérot cavity with or without attached particle P; a matrix sensor (Det) adapted to detect an image comprising a first portion (P.sub.1) deriving from the detection of the incident radiation transmitted (L.sub.TBG) by the interferometric element alone and a second portion (P.sub.2) deriving from the detection of the incident radiation transmitted (L.sub.TP) by the interferometric element and any particle (O, P) attached to a surface (Sm) of the thin layer; a processor (UT) linked to the sensor and configured: to calculate, as a function of wavelengths of the incident radiation λ.sub.i i∈[1,m], the variation of intensity of at least one first pixel of the first portion, called first variation (F.sub.BG) and of at least one second pixel of the second portion, called second variation (F.sub.P), to determine a trend, as a function of the wavelengths of the incident radiation λ.sub.i i∈[1,m], of a phase shift ϕ.sub.i between the first variation and the second variation; to detect the attached particle when the phase shift ϕ.sub.i is not constant as a function of the wavelengths of the incident radiation λ.sub.i i∈[1,m].

An inspection apparatus includes: a light source that generates and outputs light; a stage on which an inspection target is arranged; an irradiation optical system that irradiates light from the light source to the inspection target; a detector that receives the light diffracted from the inspection target and generates diffraction image; and a detector moving device configured to move the detector on a z-axis, which is an optical axis of the light, and an x-y plane perpendicular to the z-axis. Furthermore, while the detector moves on the x-y plane and the z-axis through the detector moving device, the detector generates a plurality of the diffraction images with different positions on the x-y plane and the z-axis with respect to the inspection target, and thus simultaneously implements phase retrieval and super resolution of diffraction images.

An optical inspection apparatus includes an interferometer module, which is configured to direct a beam of coherent light toward an area under inspection and to produce a first image of interference fringes of the area. The apparatus also includes a triangulation module configured to project a pattern of structured light onto the area, and at least one image sensor configured to capture the first image of interference fringes and a second image of the pattern that is reflected from the area. Beam combiner optics are configured to direct the beam of coherent light and the projected pattern to impinge on the same location on the area. A processor is configured to process the first and second images in order to generate a 3D map of the area.

It is an objective to provide a fluid quality measurement device. According to an embodiment, a fluid quality measurement device is configured to: obtain a plurality of holograms, wherein each hologram in the plurality of holograms represents a microscopic object in a fluid sample; produce a latent space representation of each hologram using a trained autoencoder neural network; assign each hologram in the plurality of holograms to a class based on the latent space representation of the hologram; and produce a fluid sample fingerprint based on the assignment of the plurality of holograms into the plurality of classes.

A non-label diagnosis apparatus for a hematologic malignancy may include a 3-D refractive index cell imaging unit configured to generate a 3-D refractive index slide image of a blood smear specimen by capturing a 3-D refractive index image in the form of the blood smear specimen in which blood (including a bone-marrow or other body fluids) of a patent has been smeared on a slide glass, an ROI detection unit configured to sample a suspected cell segment in the blood smear specimen based on the 3-D refractive index slide image and to determine, as ROI patches, cells determined as abnormal cells, and a diagnosis unit configured to determine a sub-classification of a cancer cell corresponding to each of the ROI patches using a cancer cell sub-classification determination model constructed based on a deep learning algorithm and to generate hematologic malignancy diagnosis results by gathering sub-classification results of the ROI patches.