A device including a light source, an image sensor, and a holder defining two positions between the light source and the image sensor. Each position is able to receive an object with a view to its observation. An optical system is placed between the two positions. Thus, when an object is placed in a first position, it may be observed, through the optical system, via a conventional microscopy modality. When an object is placed in the second position, it may be observed via a second lensless imagery modality.

A system for performing 3-dimensional (3-D) digital holographic refractometry includes a splitter to split a source light into a first light beam and a second light beam. A tomographic optical setup shines a sample with the first light beam and generates an image light beam. A detector array generates an interferogram signal in response to being simultaneously exposed to the image light beam and the second light beam.

Microscope (2) comprising a coherent light source (4) producing a coherent light beam (7), a light beam guide system (6) comprising a beam splitter (14) configured to split the coherent light beam (7) into a reference beam (7a) and a sample illumination beam (7b), a sample holder (18) configured to hold a sample (1) to be observed, a sample illumination device (28) configured to direct the sample illumination beam (7b) through the sample and into a microscope objective (37), a beam reuniter (16) configured to reunite the reference beam and sample illumination beam after passage of the sample illumination beam through the sample to be observed, and a light sensing system (8) configured to capture at least phase and intensity values of the coherent light beam downstream of the beam reuniter.

A microscope, a method, and a system are provided. A system includes a first optical system, a second optical system, and one or more processors. The first optical system is configured to generate an optical phase signal associated with a first image of a sample in a first field of view. The second optical system is configured to generate a polarized signal associated with a second image of the sample in a second field of view. The one or more processors is configured to generate a co-registered phase and polarization information map based on the optical phase signal and the polarized signal. The first field of view is the same as the second field of view. The first image and the second image are captured sequentially.

34 P2418PC00 Abstract Optical diffraction tomography microscope (2) comprising an illumination system (4) configured for transmitting a sample beam through a sample observation zone, a detection 5 system (8) comprising at least one image sensor (54), and a wave collection system (6) comprising a lens (16) downstream of the sample observation zone configured for directing the sample beam towards the at least one image sensor.

Optical diffraction tomography microscope (2) comprising an illumination system (4) configured for transmitting a sample beam through a sample observation zone, a detection system (8) comprising at least one image sensor (54), and a wave collection system (6) comprising a lens (16) downstream of the sample observation zone configured for directing the sample beam towards the at least one image sensor.

Provided is a determination method capable of non-destructively and simply determining a state of a sphere that is an aggregate of a plurality of cells. A phase difference image of a sphere that is an aggregate of a plurality of cells is generated from a hologram obtained by imaging the sphere, and a state of the sphere is determined on the basis of the phase difference image and a shape index value corresponding to a shape of the sphere.

Systems and methods for detecting motile objects (e.g., parasites) in a fluid sample by utilizing the locomotion of the parasites as a specific biomarker and endogenous contrast mechanism. The imaging platform includes one or more substantially optically transparent sample holders. The imaging platform has a moveable scanning head containing light sources and corresponding image sensor(s) associated with the light source(s). The light source(s) are directed at a respective sample holder containing a sample and the respective image sensor(s) are positioned below a respective sample holder to capture time-varying holographic speckle patterns of the sample contained in the sample holder. The image sensor(s). A computing device is configured to receive time-varying holographic speckle pattern image sequences obtained by the image sensor(s). The computing device generates a 3D contrast map of motile objects within the sample use deep learning-based classifier software to identify the motile objects.

An apparatus for measuring through optical means temporally resolved, optical properties, and/or phenotypes, linked to cellular homeostasis. Those temporal measurements enable the detection of cell regulation through various channels linked to homeostasis, in order to assess cell viability or early cell death through rapid diagnostic.

A digital holographic image-taking apparatus includes an illumination portion having a light emission surface for emitting illumination light toward an object, the illumination light having a specific wavelength in a coherent plane waveform; and an image sensor having an pixel array including two-dimensionally arranged pixels, the image sensor capturing an interference pattern generated based on the illumination light having acted on the object, in which the following conditional expression is satisfied: 0.0000001<Z.sup.2/S<16, where S represents the area of the light emission surface, and Z represents the distance from the light emission surface to the pixel array.