A plasmon resonance system, instrument, cartridge, and methods for analysis of analytes is disclosed. A PR system is provided that may include a DMF-LSPR cartridge that may support both digital microfluidic (DMF) capability and localized surface plasmon resonance (LSPR) capability for analysis of analytes. In some examples, the DMF portion of the DMF-LSPR cartridge may include an electrode arrangement for performing droplet operations, whereas the LSPR portion of the DMF-LSPR cartridge may include an LSPR sensor. In other examples, the LSPR portion of the DMF-LSPR cartridge may include an in-line reference channel, wherein the in-line reference channel may be a fluid channel including at least one functionalized LSPR sensor (or sample spot) and at least one non-functionalized LSPR sensor (or reference spot). Additionally, methods of using the PR system for analysis of analytes are provided.

A method for the qualitative and/or quantitative measurement of odors present in a chamber for transformation (cooking or fermentation) of a product makes it possible to prevent the measurement environment from contamination by measurement-interfering elements produced by the transformation and released in the chamber for transformation. The method makes use of a chamber for transformation, for example an oven, and of an electronic nose, pipes a fluidic system, and a removal chamber for removing the measurement-interfering elements. The method includes a measurement mode M and a cleaning mode N. The cleaning mode comprises the following steps: -N0- optionally, flushing the chamber for transformation, preferably by ventilating it; -N1- flushing the measurement chamber with a fluid, preferably ambient air; -N2- flushing the removal chamber with a fluid, preferably ambient air; and -N3- optionally, transferring at least a portion of the flushing fluid from the removal chamber into the chamber for transformation.

Particles of a sustained-release gel which is converted into a sol by reacting with a product produced by a reaction of biomolecules with an enzyme, are disposed on a measurement unit, and a measurement target biomolecule is brought into contact with the particles. Due to this contact, a product is produced according to a reaction of the biomolecule with the enzyme contained in the particles. According to the reaction with the produced product, the sustained-release is converted into a sol, and a plurality of contained detection molecules are released to the outside of the particles.

A spectroscopic analysis device includes: a film that contacts a sample subject to spectroscopic analysis; a first irradiator that irradiates a first irradiation light having transition energy to decompose attached material attached to a boundary surface of the film; and an optical waveguide that transmits the first irradiation light irradiated from the first irradiator. A first evanescent wave, based on the first irradiation light, is generated on a front surface of the optical waveguide, and is then projected on an attached region of the attached material.

A method for evaluating a test article provided by a subject for an attribute, such as a medical, industrial, veterinary, agricultural, food, or other attribute, is provided. The design includes providing a cassette comprising at least one ligand selected to match the attribute, applying the test article provided by the patient to the at least one ligand of the cassette, transmitting light energy toward the test article applied to the at least one ligand, sensing optical attributes of light energy provided from the test article applied to the at least one ligand, and providing sensed attributes of the light energy sensed to an electronic device.

A plasmon resonance (PR) system and instrument, digital microfluidic (DMF) cartridge, and methods of using localized surface plasmon resonance (LSPR) and droplet operations for analysis of analytes is disclosed. For example, a PR system is provided that may include a DMF cartridge that may support both fixed LSPR sensing capability and in-solution LSPR sensing capability for analysis of analytes. The DMF cartridge may include an electrode arrangement for performing droplet operations, wherein the droplet operations can be used for performing fixed LSPR sensing operations and in-solution LSPR sensing operations. Further, methods of using droplet operations in the DMF cartridge to perform fixed LSPR sensing operations and/or in-solution LSPR sensing operations are provided.

A prism (1090) is configured from a dielectric medium and is used in analysis using surface plasmons. The prism (1090) is provided with an incidence surface (1170) on which excitation light from outside is incident, a reflection surface (1172) on which excitation light having entered the incidence surface (1170) is reflected, an emission surface (1174) from which excitation light reflected by the reflection surface (1172) is emitted, and an opposing surface (1175) opposing the reflection surface (1172). A gold film (1092) is formed on the reflection surface (1172). The opposing surface (1175) has a sink-mark surface (1200), and the sink-mark surface (1200) is a transparent surface.

The disclosure provides a method of generating an artificial fluorescent image of cells is provided. The method includes receiving a brightfield image generated by a brightfield microscopy imaging modality of at least a portion of cells included in a specimen, applying, to the brightfield image, at least one trained model, the trained model being trained to generate the artificial fluorescent image based on the brightfield image, receiving the artificial fluorescent image from the trained model

An integrated spectro-microscopic system for multimodality imaging on a sample includes a reflected differential interference contrast (RDIC) microscope, a Raman spectroscope optically coupled with the RDIC microscope and a total internal reflection fluorescence/scattering (TIRF/TIRS) microscope optically coupled with the RDIC microscope such that the integrated spectro-microscopic system is capable of simultaneously acquiring both the RDIC images, the Raman spectra and TIRF/TIRS images on the same sample.

The invention(s) cover a sensor and method of fabrication, the sensor including: a substrate; and a distribution of nanoparticles patterned onto the substrate as a set of regions. In variations, the sensor 100 can further include one or more channels in fluid communication with the distribution of nanoparticles. In variations, different nanoparticle regions can be optionally functionalized with different probe molecules in order to provide additional functionality with respect to the assay(s) being performed using the sensor 100. Additionally or alternatively, in variations, unoccupied regions of the substrate 110 and/or nanoparticle surfaces can optionally include passivated surfaces to prevent non-specific binding, without significantly shifting the LSPR wavelength, in order to significantly improve signal-to-noise ratio (SNR) provided by the sensor. The sensor can be used for performance of multiplexed assays (e.g., for infectious disease panels) with processing of different types of sample material.