The present invention relates to a method and system for identifying mechanical properties of a cell nucleus through a label-free cell analysis based on Brillouin light scattering techniques. The present application additionally provides a method and system for identifying cancerous cells based on mechanical properties of the cell nucleus.

Disclosed is an acoustic resonance spectrometry system for analysing a sample, which includes an optical pump-probe device adapted to generate a pump beam and a probe beam, the pump beam being consisted of a series of ultra-short pump light pulses having a repetition frequency in the spectral domain of the gigahertz, the pump beam being directed towards an optoacoustic transducer to generate a periodic grating of coherent acoustic phonons in the sample, the probe beam being directed towards the sample to form a scattering beam of the probe beam on the grating of phonons, a frequency variation device being adapted to vary the repetition frequency of the pump beam in a spectral range and a photo-detection system configured to measure a scattering signal as a function of the repetition frequency in the spectral range.

In a measurement requiring a high space resolution using S-BOTDR, a pulse train composed of a plurality of pulses having the interval between the pulses longer than the phonon lifetime is interpulse-code-modulated. A Golay code is used for the interpulse code modulation to eliminate the sidelobes of the correlation in using a technique of correlation. In a technique without using correlation, an Hadamard matrix is used for the interpulse code modulation and the resultant matrix is inverted in the signal processing.

The present invention relates to a method and system for a label-free cell analysis based on Brillouin light scattering techniques. Combined with microfluidic technologies according to the present invention, Brillouin spectroscopy constitutes a powerful tool to analyze physical properties of cells in a contactless non-disturbing manner. Specifically, subcellular mechanical information can be obtained by analyzing the Brillouin spectrum of a cell. Furthermore, a novel configuration of Brillouin spectroscopy is provided to enable simultaneous analysis of multiple points in a cell sample.

A system and method are provided for distributed acoustic sensing in a multimode optical fiber. The system includes a transmitter for simultaneously propagating a sequence of M light pulses through the multimode optical fiber using a spatial mode selected from a set of N spatial modes provided by a spatial mode selector for the transmitter that is coupled to an input to the multimode optical fiber, with M and N being respective integers greater than one. The system further includes a receiver for receiving the sequence of M light pulses at an output of the multimode optical fiber and detecting an environmental perturbation in the multimode optical fiber based on an evaluation of a propagation of the sequence of M light pulses through the multimode optical fiber.

Arrangements and methods are provided for obtaining informationassociated with an anatomical sample. For example, at least one first electro-magnetic radiation can be provided to the anatomical sample so as to generate at least one acoustic wave in the anatomical sample. At least one second electro-magnetic radiation can be produced based on the acoustic wave. At least one portion of at least one second electro-magnetic radiation can be provided so as to determine information associated with at least one portion of the anatomical sample. In addition, the information based on data associated with the second electro-magnetic radiation can be analyzed. The first electro-magnetic radiation may include at least one first magnitude and at least one first frequency. The second electro-magnetic radiation can include at least one second magnitude and at least one second frequency. The data may relate to a first difference between the first and second magnitudes and/or a second difference between the first and second frequencies. The second difference may be approximately between 100 GHz and 100 GHz, excluding zero.

A diagnosis system and a diagnosis method are provided. More specifically, embodiments of the present disclosure relate to a diagnosis system for detection of corneal degeneration impacting the biomechanical stability of the human cornea and a diagnosis method for detection of corneal degeneration impacting the biomechanical stability of the human cornea. Still more specifically, embodiments of the present disclosure relate to a diagnosis system for early detection of corneal degeneration impacting the biomechanical stability of the human cornea and a diagnosis method for early detection of corneal degeneration impacting the biomechanical stability of the human cornea.

The present invention relates to a method and system for a label-free cell analysis based on Brillouin light scattering techniques. Combined with microfluidic technologies according to the present invention, Brillouin spectroscopy constitutes a powerful tool to analyze physical properties of cells in a contactless non-disturbing manner. Specifically, subcellular mechanical information can be obtained by analyzing the Brillouin spectrum of a cell. Furthermore, a novel configuration of Brillouin spectroscopy is provided to enable simultaneous analysis of multiple points in a cell sample.

Disclosed is an acoustic resonance spectrometry system for analysing a sample, which includes an optical pump-probe device adapted to generate a pump beam and a probe beam, the pump beam being consisted of a series of ultra-short pump light pulses having a repetition frequency in the spectral domain of the gigahertz, the pump beam being directed towards an optoacoustic transducer to generate a periodic grating of coherent acoustic phonons in the sample, the probe beam being directed towards the sample to form a scattering beam of the probe beam on the grating of phonons, a frequency variation device being adapted to vary the repetition frequency of the pump beam in a spectral range and a photo-detection system configured to measure a scattering signal as a function of the repetition frequency in the spectral range.

Arrangements and methods are provided for obtaining information associated with an anatomical sample. For example, at least one first electro-magnetic radiation can be provided to the anatomical sample so as to generate at least one acoustic wave in the anatomical sample. At least one second electro-magnetic radiation can be produced based on the acoustic wave. At least one portion of at least one second electro-magnetic radiation can be provided so as to determine information associated with at least one portion of the anatomical sample. In addition, the information based on data associated with the second electro-magnetic radiation can be analyzed. The first electro-magnetic radiation may include at least one first magnitude and at least one first frequency. The second electro-magnetic radiation can include at least one second magnitude and at least one second frequency. The data may relate to a first difference between the first and second magnitudes and/or a second difference between the first and second frequencies. The second difference may be approximately between 100 GHz and 100 GHz, excluding zero.