Device and methods for detection and classification of pathogens have an imaging module, an image processing module, and a display module. The imaging module has a plurality of light sources to expose a sample to excitation radiation at various wavelengths. A detector in the imaging module synchronously captures time-resolved fluorescence emission spectra, time-resolved reflectance, and transmittance spectra at multiple spectral bands from the sample. The image processing module resolves the spectra and compares obtained spectral parameters to set of standard parameters provided in a library database to determine a match to detect and classify pathogens.

A measuring method for measuring dissolved oxygen includes performing a first measurement sequence, including: emitting a first stimulation signal onto a sensor for a first period; detecting a first detection signal; determining a phase shift between the first stimulation signal and the first detection signal; and calculating a first measured value based on the determined phase shift. Performing a second measurement sequence, including a second stimulation signal onto the sensor for a second period, wherein the second stimulation signal is different than the first stimulation signal; detecting a second detection signal; determining a decay time of the second detection signal; calculating a second measured value based on the decay time. The method further includes comparing the first measured value to the second measured value and correcting the first measured value when a difference between the first measured value and the second measured value is greater than a first limit value.

A passive, hydrodynamic technique implemented using a microfluidic device to perform co-encapsulation of samples in droplets and sorting of said droplets is described herein. The hydrodynamic technique utilizes laminar flows and high shear liquid-liquid interfaces at a microfluidic junction to encapsulate samples in the droplets. A sorting mechanism is implemented to separate sample droplets from empty droplets. This technique can achieve a one-one-one encapsulation efficiency of about 80% and can significantly improve the droplet sequencing and related applications in single cell genomics and proteomics.

A ternary fluorescence correlation spectroscopy system for analyzing an interaction between three kinds of molecules, including at least three excitation light sources with different wavelengths. The excitation light sources are configured to illuminate and excite a sample to generate a fluorescence signal. The fluorescence signal is divided into multiple signals according to wavelength, which are then respectively detected by a single photon detector and transmitted to a signal acquisition and operation card to perform real-time operation of a triple-correlation function, so as to obtain a fluorescence triple-correlation spectroscopy curve.

Embodiments described herein generally relate to: sensing and/or authentication using luminescence imaging; diagnostic assays, systems, and related methods; temporal thermal sensing and related methods; and/or to emissive species, such as those excitable by white light, and related systems and methods.

The present invention relates to a method for analyzing and selecting a specific droplet among a plurality of droplets (4), comprising the following steps: —providing a plurality of droplets (4), —for a droplet (4) among the plurality of droplets, measuring at least two optical signals, each optical signal being representative of a light intensity spatial distribution in the droplet for an associated wavelength channel, —calculating a plurality of parameters from the optical signals, —determining a sorting class for a droplet according to calculated parameters, —sorting said droplet according to its sorting class, wherein the plurality of parameters comprises the coordinates of a maximum for each optical signal and a co-localization parameter and the at least two calculated parameters used for the determining step comprises the co-localization parameter.

A method may include maintaining a sample comprising an ionic species and an optical indicator at an elevated temperature above 25° C. on a semi-conductive microfluidic die during an incubation period, intermittently interrogating the sample with an interrogating light during the incubation period and sensing a response of the sample to the interrogating light, wherein the sample is interrogated with the interrogating light only during those times at which the sample is being sensed.

Digital assay system, including methods, apparatus, and compositions, for assay of one or more targets in a set of partitions containing a generic reporter of target amplification.

A miniaturized chromatographic inspection apparatus capable of optimal light emission and reading control according to the type of marker, and a control method thereof. The apparatus can comprise: a main body; a cartridge tray with a seated immunochromatographic test cartridge, configured to be accommodated in the main body or to be withdrawn to the outside of the main body; a tray driving unit for moving the cartridge tray; an image sensing unit for recognizing a code expression provided on a surface of the cartridge or excitation light generated from the cartridge; a first light source illuminating the code expression provided on the surface of the cartridge; a second light source illuminating a window of the cartridge to generate the excitation light in the cartridge; and a control unit for controlling the tray driving unit, the first and second light source, and processing information acquired from the image sensing unit.

Certain embodiments provide a marker for detecting a detection target substance, including an assay reagent having a medium, and a composition containing a solvent or a solvent mixture, and a fluorescent coagulation factor that has a fluorescence property that changes as it coagulates in presence of the detection target substance. A difference of a hydrophobicity parameter LogP between the solvent and a part or the entirety of the detection target substance is equal to or smaller than 1.53.