A sensor network, which includes a sensor controller serially coupled to a plurality of sensor modules, is configured to program the sensor modules so as to transfer measurement data to the sensor controller and to synchronize the sensor modules to picosecond accuracy via on-chip or on-module custom circuits and a physical layer protocol. The sensor network has applications for use in PET, LiDAR, FLIM and flow cytometry applications. Synchronization, within picosecond accuracy, is achieved through use of a picosecond time digitization circuit. The picosecond time digitization circuit is used to measure on-chip delays with high accuracy and precision. The delay measurements are directly comparable between separate chips even with voltage and temperature variations between chips.

Swept polarization optical beams are directed to a sample to produce fluorescence. typical as a plurality of single photon detection events. Based on frequencies associated with the polarization sweeps, orientation of a sample can be determined. Using polarization sweeps at first and second frequencies and wavelength, donor fluorophore orientation can be established based on the first frequency and wavelength and acceptor orientation can be established based on a sum or difference of the first and second frequencies and the second wavelength.

A method for detecting a photochemically active chemical species in a sample, comprising the steps of: a) illuminating the sample with light at a wavelength suitable to trigger a reaction affecting an optical property of the chemical species according to an illumination sequence, such that: in at least a first time window, the kinetics of the first reaction is limited by a photochemically-activated step of the reaction; and in at least a second time window, the kinetics of the first reaction is limited by a thermally-activated step; b) measuring the evolution of the optical property during the first and the second time windows; c) determining at least a first and a second time constant representing the kinetics of the first reaction in the first and the second time windows, respectively; and d) using the determined time constants for identifying the chemical species. An apparatus for carrying out such a method.

The present invention relates to an apparatus for performing a nucleic acid amplification reaction and a fluorescence detection device for reaction analysis. The nucleic acid amplification apparatus of the present invention uses a plurality of blocks having different reaction temperatures by independent temperature control and the movement between the blocks is performed along sliding recesses formed in the blocks, enabling to greatly shorten the total amplification time (TAT). In the fluorescence detection device of the present invention, the positions of the light source and the photodetector are very unique for the reaction vessel in which an excitation light is provided and an emission light is generated.

A magnetic measuring device includes: a determination part configured to identify four maximum inclination points in an average value in a visual field of a light detection magnetic resonance spectrum and configured to determined a degree of decrease in relative fluorescence intensity and a microwave frequency at each of the maximum inclination points; a setting part configured to set a reference decrease degree of the relative fluorescence intensity in a predetermined area and configured to set operating point frequency initial values at four points at which the reference decrease degree is achieved, near the microwave frequencies at the respective maximum inclination points; a frequency update part configured to update operating point frequencies at the four points; and a frequency correction part configured to input the updated operating point frequencies to a microwave oscillator as corrected operating point frequencies.

The present invention relates to a real-time fluorescence imaging sensor for measuring glutathione in cell organelles and a method for fabricating the same. More specifically, the present invention relates to a novel compound for measuring glutathione in cell organelles, a method for preparing the novel compound, a real-time fluorescence imaging sensor for measuring glutathione in cell organelles, which comprises the novel compound, a method for fabricating the imaging sensor, and a method of measuring glutathione in cell organelles by use of the imaging sensor. When the composition comprising the compound according to the present invention is used, it can measure the antioxidant activity of the organelle mitochondria or Golgi apparatus in living cells, particularly stem cells, and can screen highly active stem cells based on the results obtained by measuring the antioxidant activity of the cell organelle.

Among other things, an imaging device has a photosensitive array of pixels, and a surface associated with the array is configured to receive a specimen with at least a part of the specimen at a distance from the surface equivalent to less than about half of an average width of the pixels.

Spectrophotometer system configured to characterize and/or measure spectrally (wavelength)-dependent properties of material components (such as molecular, viral, and/or bacterial analytes) associated with or of an object prior to the time when optical fingerprints of such material components start to degrade, and associated methods. System can be enhanced by a capability of selecting specific wavelengths of operation for such system to optimize cost-efficiency of the system.

The present disclosure in some aspects provides methods for the controlled merging of emulsion droplets, which can be used to assemble useful compositions such as droplets (e.g., stabilized micelles) containing a precise combination of analytes and/or analytical reagents. In some embodiments, disclosed herein is a method, e.g., for detecting the presence/absence, a level or amount, and/or an activity of an analyte in a sample, comprising merging two or more emulsion droplets such that an interaction between an analyte and an analyte-interacting reagent occurs in the merged droplet. The two or more emulsion droplets may be merged using a method for the controlled merging of emulsion droplets disclosed herein.

The present invention discloses a time-resolved immunoquantitation test strip for detecting tetrodotoxin (TTX) in shellfish food, which belongs to the technical field of rapid detection of time-resolved immunoassay. In the present invention, fluorescent microspheres are adopted to replace the traditional colloidal gold; the fluorescent microsphere is used for labeling a TTX antibody complex; by utilizing a competitive immunization method, the fluorescent microsphere, serving as a fluorescent probe, is used for immunochromatography; and by reading the fluorescence value of a detection line on a fluoroimmunoassay instrument, the TTX in shellfish samples can be analyzed quantitatively and rapidly. The time-resolved immunoquantitation test strip of the present invention can be used for detecting the content of the TTX in various types of shellfish food quantitatively and rapidly and is strong in specificity and high in sensitivity, wherein when the concentration of the TTX is 0.5-40 ng/mL, the logarithmic value of the concentration has a linear relationship with T/To, a linear equation is: Y=0.57365-0.2668LgX, R.sup.2=0.9940, and the limit of detection can reach 0.047 ng/mL.