An LED spectrofluorometer (100) for analysis of an object (101) includes a light excitation element (11, 112, 113) suitable for illuminating a study zone (101B) of the object with an excitation light beam (1), and an optical routing element (121, 122, 123, 124) suitable for collecting a fluorescent light flux (2) emitted by the study zone excited by the excitation light beam and for routing the fluorescent light flux to an optical spectrometer (131) for analysis of the light spectrum thereof. The light excitation element includes a first light-emitting diode (111) and a second-light emitting diode (112), the first light-emitting diode emitting at a first wavelength (λ1) between 250 and 300 nm and the excitation light beam being formed from one or other of the light beams generated by each light-emitting diode.

The disclosed embodiments relate to a system that displays an image of the characteristics of the biological tissue. During operation, the system enables a user to illuminate a measurement location in an area of interest on the biological tissue by manipulating a point measurement probe, wherein the point measurement probe delivers both an excitation beam and an overlapping aiming beam that is visible to a camera. Next, the system obtains fluorescence information from a fluorescence signal emitted from the measurement location in response to the excitation beam. The system then captures an image of the area of interest using the camera and identifies a portion of the image that corresponds to the measurement location by identifying a location illuminated by the aiming beam. Finally, the system generates an overlay image by overlaying the fluorescence information onto the portion of the image that corresponds to the measurement location, and then displays the overlay image to a user.

A convective polymerase chain reaction apparatus and an optical detecting method thereof are provided. The optical detecting method includes the following steps. A substance in a tube to be tested is heated. At least two monochromatic lights are provided and are combined using a light combining element to irradiate the tube to be tested. At least two excited lights generated by exciting the substance in the tube to be tested by the at least two monochromatic lights are sensed.

There is described a fiber-optic sensor for measuring a light signal from a fluorescible sample comprising heavy metal ions, for example. The fiber-optic sensor comprises an optical fiber having a side surface by which the light signal from the fluorescible sample is inputted. The optical fiber is corrugated to form at least two gratings on the side surface of the optical fiber. Each grating comprises periodically longitudinally spaced-apart valleys on the surface of the optical fiber, and is longitudinally spaced apart from any other grating of the at least two gratings.

The present invention relates to a method for analyzing and selecting a specific droplet among a plurality of droplets (4), comprising the following steps: —providing a plurality of droplets (4), —for a droplet (4) among the plurality of droplets, measuring at least two optical signals, each optical signal being representative of a light intensity spatial distribution in the droplet for an associated wavelength channel, —calculating a plurality of parameters from the optical signals, —determining a sorting class for a droplet according to calculated parameters, —sorting said droplet according to its sorting class, wherein the plurality of parameters comprises the coordinates of a maximum for each optical signal and a co-localization parameter and the at least two calculated parameters used for the determining step comprises the co-localization parameter.

The present invention relates to methods of nucleic acid analyte detection by PCR. In particular, methods and kits for the detection of a plurality of nucleic acid analytes and the generation of kinetic signatures are provided. Further provided are methods and kits of nested PCR and PCR using limiting primers.

Disclosed is a method of recovering a peptide, including: mixing a liquid sample containing a complex of peptide and protein in blood with a reagent containing a neutral amino acid, an acidic amino acid or both the neutral and acidic amino acids to liberate the peptide from the protein in blood; and recovering the liberated peptide.

Systems and methods are provided for sample processing. A device may be provided, capable of receiving the sample, and performing one or more of a sample preparation, sample assay, and detection step. The device may be capable of performing multiple assays. The device may comprise one or more modules that may be capable of performing one or more of a sample preparation, sample assay, and detection step. The device may be capable of performing the steps using a small volume of sample.

A library of known intrinsic spectra is provided to identify at least one known material in a sample of interest. The library includes individual intrinsic spectra channels defined by the assignment of intrinsic spectra of at least one known material, and combinations thereof, so that the assigned intrinsic spectra of each intrinsic spectra channel is correlated to at least one known material. The at least one known material is identified in the sample of interest when intrinsic spectra obtained from the sample of interest is matched to an assigned intrinsic spectra of an intrinsic spectra channel of the library of known intrinsic spectra.

Detection systems and methods of use thereof, and, in particular, hyperspectral fluorescence systems and methods for detecting aerosolized viral biologics and/or carcinogenic compounds or elements. The system utilizes, and the method is, a non-invasive, immediate, and non-biohazard byproduct method (with the actual imaging to determination generally occurring in less than 3 seconds) with a throughput of the station in around ten seconds (which is allowing for sanitization of the station before and after each individual is tested).