A discrimination system that forms a fluid column and interrogates objects within the fluid column with an excitation source. An optical arrangement collects output electromagnetic radiation emanating from the excited objects disposed within the fluid column and directs the output electromagnetic radiation to a detector. An analyzer reduces the positional dependency of the detected intensity by normalizing the value based on the position of each object.

A biological tissue analyzing device configured to analyze a biological tissue using hyperspectral data in which spectral information is associated with each of pixels forming a two-dimensional image and comprising the following (i) and (ii), as well as comprising (iii) and/or (iv): (i) a hyperspectral data acquisition unit configured to acquire the hyperspectral data; (ii) an analysis target region extraction unit configured to extract pixels corresponding to an analysis target region from a two-dimensional image of the biological tissue; (iii) an altered state classification unit configured to roughly classify an altered state of the biological tissue with unsupervised learning; and (iv) an altered state identification unit configured to identify the altered state of the biological tissue with supervised learning.

An imaging system includes an imaging device having a holder configured to hold a cell culture plate with a plurality of wells. The imaging device also includes an imaging assembly having a plurality of imaging units, each of which is configured to image one well of the plurality of wells. The imaging system also includes a storage platform in communication with the imaging device configured to receive a plurality of images from the imaging device. The system further includes a computer in communication with the imaging device and the storage platform. The computer is configured to control the imaging device and to display at least one image of the plurality of images.

The present invention is directed to an apparatus (10) for reliable quantitative measurement of a fluorescent blood analyte in tissue (12) comprising: at least one light source (14), the light source (14) emitting excitation light at least at a first wavelength range between 350 nm and 450 nm to the tissue (12); a detection unit (16), the detection unit (16) measuring: a) a portion of the fluorescent light emitted by the fluorescent blood analyte excited at the first wavelength range; and b) a portion of the auto fluorescence emitted by the tissue (12); and/or c1) a portion of the remitted excitation light at the first wavelength range, and c2) a portion of the remitted light at a second wavelength range; and a control unit (18), the control unit (18) operating the light source (14) and detection unit (16). The present invention is further directed to a method for quantitative measurement of a fluorescent blood analyte in tissue (12).

Provided herein are compounds, compositions, kits, uses, and methods for assessing the viability of cells and/or quantifying the amount of live and dead cells in a mixture using differential stains. In some embodiments, the compounds are used in culture media or can function independently of fixation and/or permeabilization. In some embodiments, the compounds comprise a platinum atom. In some embodiments, the compounds comprise a nitrogen mustard moiety.

A mold sensor is configured with an enclosed chamber in which a nutrient-treated substrate is positioned. The mold sensor includes a sensing system that is configured to measure properties of pressure waves within the enclosed chamber. A controller operates the sensing system and is programmed to detect a presence of mold growing in the chamber based on the characteristics of the pressure wave response within the chamber.

Some embodiments of the methods provided herein relate to sample analysis and particle characterization methods for large, multi-parameter data sets. Frequency difference gating compares at least two different data sets to identify regions in a multivariate space where a frequency of events from a first data set is different than a frequency of events from the second data set according to a defined threshold.

The invention provides new cyanine dyes for staining living cells, providing fluorescence emission in red, green and yellow, thus allowing “multichannel” staining. The dyes are binding to nucleic acids and allow the observation of the stained cells, for the staining does not negatively interfere with the viability of the stained cells. The inventive dyes thus can advantageously be used for pathogen-host investigations or any other type of investigation of cell-cell-interactions, for the natural behavior of the stained cells (microorganisms, pathogens etc.) can easily be observed.

A method of imaging described herein comprises (a) disposing ultrasound-switchable fluorophores within an environment; (b) exposing the environment to ultrasound to create a first activation region within the environment; (c) disposing the fluorophores within the first activation region to switch the fluorophores from an off state to an on state; (d) irradiating the environment to excite the fluorophores within the first activation region; (e) detecting photoluminescence from the excited fluorophores at a first optical spot on an exterior surface of the environment; (f) subsequently creating a second activation region within the environment; (g) switching fluorophores within the second activation region to an on state; (h) exciting the fluorophores in the on state within the second activation region; and (i) detecting photoluminescence from the excited fluorophores within the second activation region at a second optical spot on the exterior surface, wherein the first and second optical spots are optically resolvable.

A system and method for mapping metabolic data of a heart. The system has a light source directing light onto the heart, one or more lenses for focusing an image of the heart, and a fluorescent detector receiving the focused image and generating transients and/or waves to map metabolic cardiac data.