In a method for measuring at least two different components of a fluid, the fluid is to a first measuring cell and a second measuring cell. In the first measuring cell, a first component of the fluid is excited by a first excitation to trigger a first light emission, and in the second measuring cell, a second component of the fluid is excited by a second excitation which is different from the first excitation, thereby triggering a second light emission. The first light emission and the second light emission are captured by an optical system facility and guided by the optical system facility in a direction of a detector facility which measures the first light emission and the second light emission.

A three-dimensional Raman image mapping measuring device for a flowable sample according to an embodiment of the present disclosure is designed to be capable of measuring a flowable sample during mapping measurement of a three-dimensional image that is a region of a confocal Raman by using a micro Raman spectrometer and a three-axis sample stage (Piezo stage). The three-dimensional Raman image mapping measuring device for a flowable sample includes at least one piezo element; an element holder equipped with the piezo element and having an opening, a sample stage for supporting the element holder equipped with the piezo element, an objective lens mounted in the opening in the element holder, a sample holder for controlling vertical movement of the flowable sample disposed under the lower portion of the sample stage, and a transparent window disposed between the sample stage and the sample holder.

A method for detecting and quantifying an amount of ammonia in a sample by surface-enhanced Raman spectroscopy includes a step of position a liquid or gaseous sample proximate to a detection substrate. Incident light is focused onto the detection substrate while it is positioned proximate to the sample, the incident light having an excitation wavelength from about 500 nm to 800 nm. Raman activity from ammonia proximate to the detection substrate is then detected.

A plasmonic sensor, having at least a substrate, a laser processed active surface area on the said substrate, and a metal coating on the activate surface, where the laser processed surface is fabricated by means of short laser pulses in such a way that in a shallow layer of the surface material, the viscosity is reduced and under the influence of the same pulse, which was used to reduce the viscosity, or a successive incident one or more pulses a self-organized, stochastic nanostructure is formed, which has features smaller than 1 μm. In some implementations, the substrate material is amorphous, such as soda-lime glass or similar. Also disclosed is a slide and/or a slip cover, which are used in microscopy, for forming the active sensor area on top surface of it.

A system for analysis of vent gas of a urea plant, comprising: a Raman spectroscope; a sampling conduit that connects the spectroscope to a main pipe of the urea plant configured to convey a sample stream to be analysed to the spectroscope; and a temperature-adjusting device, operated by a temperature controller and acting on at least one thermal treatment portion of the conduit configured to adjust the temperature of the sample stream circulating in the conduit.

An apparatus (10) for characterizing particles, comprising: a microscope objective with an optical axis and a depth of field; a holder cell (22) configured to position the particles in a generally planar volume below the microscope objective, the planar volume being substantially normal to the optical axis and having a depth that is less than or equal to the depth of field, wherein a portion of the cell holder (22) for positioning in the optical axis of the microscope objective is substantially free of significant spectral features in a Raman spectral range; an x-y stage (20) to move the microscope objective relative to the holder cell (22) in x and y directions to align particles with the optical axis of the microscope objective while the particles are held by the holder cell (22), a detector (18) for acquiring an image of a particle through the microscope objective, a laser operable to illuminate a particle held by the holder cell (22), a Raman spectrometer (16) arranged to obtain a spectrum including the Raman spectral range from the illuminated particle, and characterizing logic operative to characterize the particle based on image processing operations performed on the acquired image and based on the Raman spectrum. The holder cell (22) comprises a first plate (34) and a second plate (36) that are separated by a predetermined distance defining the planar volume depth.

There is a need in the point-of-care diagnostic community for an efficient and portable method for testing blood and other biological fluids that can be easily translated across multiple applications. An aspect of the invention described involves monitoring the optical properties of molecularly-mediated nanoparticle assemblies though an optically transparent and magnetically active microfluidic chip, which has recently emerged as an attractive method for biomarker detection as it is an efficient tool for monitoring the binding events that take place in a sensing assay. In one embodiment, this device is directed towards two-nanoparticle assays that rely on the assembly or disassembly of plasmonic and magnetic nanoparticles in response to a certain analyte. A further embodiment is directed to a spiral microfluidic using inertial forces to filter fluid components by size, connected to a magnetically active channel comprised of a nickel micropad array, optically transparent microchannel, and permanent magnets.

Various techniques are provided to implement, operate, and manufacture a chemical detection device. In one embodiment, a device includes a flow path comprising an analyte reporter configured to receive samples passed by the flow path. The device also includes an excitation source configured generate a response from the analyte reporter. The device also includes a detector configured to receive the response from the analyte reporter to determine whether the samples comprise a material of interest. The device also includes a support structure configured to position the flow path relative to the excitation source and the detector, wherein the support structure comprises a carbon filled polymer material. Additional devices, systems, and methods are also provided.

A surface-enhanced Raman scattering unit includes a surface-enhanced Raman scattering element including an optical functional portion that causes surface-enhanced Raman scattering, and a support member that supports the surface-enhanced Raman scattering element. The surface-enhanced Raman scattering element is fixed to the support member due to a magnetic force.

A method for enhancement of spontaneous Raman scattering (SRS) from gases comprising a multimode blue laser diode which receives feedback from a near concentric bidirectional multipass cavity in such a way as to generate a circulating power of order 100 W for a sample volume of 10 mm.sup.3. The feedback, provided via a volume Bragg grating, reduces the laser bandwidth to 4 cm.sup.−1. Spectra of spontaneous Raman scattering from ambient atmospheric air, detected collinearly with the pump, were recorded with a limit of detection below 1 part-per-million.