Apparatuses, systems, and methods for Raman spectroscopy are described. In certain implementations, a spectrometer is provided. The spectrometer may include a plurality of optical elements, comprising an entrance aperture, a collimating element, a volume phase holographic grating, a focusing element, and a detector array. The plurality of optical elements are configured to transfer the light beam from the entrance aperture to the detector array with a high transfer efficiency over a preselected spectral band.

An adaptation device is provided for adapting an UV-Vis cuvette to perform in-situ spectroanalytical characterization of redox processes using gas species or solutes in a controlled atmosphere, the device configured to fit the open end of a UV-Vis cuvette intended to contain products to be measured during achievement of spectroanalytical measurements. The device comprises a main body configured to form a lid covering the opened end of the cuvette, the main body having a first part and a second part; a working, a counter and a reference electrode with removable parts at the respective ends of three conductors coming from outside and passing through the main body; a gas inlet to allow introduction of a gas the gas inlet having one aperture directed to the working electrode and second aperture directed to the bottom of the cuvette, a gas outlet intended to let the reactive gas in excess to flow out of the cuvette and an solution inlet tube for titration measurements.

A micro-fluidic system comprising means for optically trapping a particle and a Raman excitation source for causing Raman scatter from the particle whilst it is in the optical trap.

Examples include a fluid device. The fluid device includes a substrate, a sensor coupled on the substrate. A reservoir is formed in the substrate adjacent to the sensor. A deformable cover is disposed to seal the sensor and the reservoir on the substrate.

Disclosed herein are devices comprising: a sample conduit providing a path for fluid flow extending from a sample inlet to a sample outlet; a thermal housing enclosing the sample conduit, wherein the thermal housing comprises a plurality of measurement regions; and a motorized stage translatable along the thermal housing from a first location to a second location so as to align a detector with one or more of the plurality of measurement regions. Also disclosed are methods of use of the devices described herein.

A flow cell device including a spherical optical element is disclosed. The spherical lens can be sealed to the body of the flow cell device in a manner that provides external optical access to a fluid in an analysis region of a flow path through the flow cell device. The seal can be provided by an elastomer, a polymer, or a deformable metal. The disposition of the spherical lens to the flow path enables in situ optical analysis of the fluid. An optical analysis device can be removably connected to the flow cell device to provide the optical analysis. In some embodiments the optical analysis device is a portable Raman spectrometer. The flow cell device can provide a supplementary interrogation interface, and/or an on board sensor device(s) to enable multivariate analysis and/or advanced triggering.

A spectroscopically active nano-particle assembly is provided. The nano-particle assembly includes a cluster of metallic nano-particles. A first protective coating is formed over a first side of the cluster, and a second protective coating is formed over a second side of the cluster, wherein the second side of the cluster is opposite the first side.

Particle detection cartridges are provided. Aspects of the particle detection cartridges according to certain embodiments include a sample input, a flow channel and a light channel, where the flow channel and light channel are coupled at a detection region such that only light from the detection region can propagate directly through the light channel to a detector. Systems including the cartridges, as well as methods for detecting particles in a sample with the subject particle detection cartridges/systems, are also described. Kits having one or more cartridges are also provided.

An apparatus includes a sample carrier having wells. Each of the wells has sides and a floor forming an interior. A surface enhanced Raman spectroscopy (SERS) structure is within the interior of each of the wells. A pneumatic port is connected to the interior of each of the wells. A pneumatic passage is connected to the pneumatic ports.

A Raman spectroscopic measurement system for measuring the material composition of a mixed phase fluid having a gas phase dispersed in a liquid phase or vice versa is disclosed, which includes an insert to be inserted into a process. The insert includes a measurement chamber partially defined by a phase separating membrane that enables the gas phase to diffuse into and out of the measurement chamber and facilitates coalescing of the liquid phase which into a collector. A first probe of the measurement system is configured to transmit excitation light into the measurement chamber and to receive a Raman signal emanating from the gas phase therein, and a second probe is configured to transmit excitation light into the drain and to receive a Raman signal emanating from the liquid phase therein. The measurement system further includes a spectrometer to determine the material composition of the fluid from the Raman signals.