A method for coherent multidimensional spectroscopy may comprise illuminating a location in a sample with a set of m coherent light pulses, each coherent light pulse having an initial frequency ω.sub.m and an initial wave vector {right arrow over (k)}.sub.m, wherein m≥2, to generate a coherent output signal having an initial frequency ω.sub.output=Σ±ω.sub.m and an initial wavevector wave vector {right arrow over (k)}.sub.output=Σ±{right arrow over (k)}.sub.m; scanning a first coherent light pulse of the set of m coherent light pulses across a set of i frequency values, wherein i≥2, the set of i frequency values including the first coherent light pulse having initial frequency ω.sub.1; scanning, simultaneously, a second coherent light pulse of the set of m coherent light pulses across a set of i correlated frequency values, the set of i correlated frequency values including the second coherent light pulse having initial frequency ω.sub.2, wherein each correlated frequency value is associated with a corresponding frequency value of the set of i frequency values as a correlated frequency grouping; and detecting the coherent output signal. Each correlated frequency value is selected so that the coherent output signal generated at each correlated frequency grouping equals the initial frequency ω.sub.output and the coherent output signal generated at each correlated frequency grouping equals the initial wavevector {right arrow over (k)}.sub.output.

A system for measurement is provided. The system comprises a core optical module and a scanning interface module. The core optical module is configured to generate a light for generating signals for analyzing an object through the scanning interface module and detect a light including the signals from the object through the scanning interface module. The scanning interface module is changeable for each application and configured to connect with the core optical module by a light transferring unit to scan the object with the transferred light from the core optical module and to receive the light from the object to transfer to the core optical module.

There is provided an apparatus including a chip containing metal bodies capable of exciting localized surface plasmon resonance at a first surface, and an analyzer unit that performs a scan of the first surface of the chip, in a state where the first surface is in contact with a sample, with a laser in at least a one-dimensional direction and records scattered light, which has been enhanced at the first surface, in association with the scan. The chip includes a substrate, a first layer where concave and convex structures are repeatedly provided on the first surface of the substrate; and a second layer that contains the metal bodies and is provided via the first layer.

A coherent anti-Stokes Raman scattering microscope imaging apparatus, comprising: a laser light source (21), a two-dimensional oscillating mirror assembly (22), a first light dichroic mirror plate (23), an objective lens (24), a sample translation platform (25), a collection device (26), and a data processing module; the laser light source (21) is used for producing a first laser beam and a second laser beam; the first laser beam and the second laser beam are coaxially emitted; the first laser beam and the second laser beam are incident on the two-dimensional oscillating mirror assembly (22), and the two-dimensional oscillating mirror assembly (22) adjusts the optical path of the first laser beam and the second laser beam; the first laser beam and the second laser beam leaving the two-dimensional oscillating mirror assembly pass in sequence through the first light dichroic mirror plate (23) and the objective lens (24); the objective lens (24) focuses the first laser beam and the second laser beam onto the sample translation platform; the signal light produced on the sample translation platform (25) passes through the objective lens (24), and the collection device (26) produces initial data on the basis of the signal light, and outputs the initial data to the data processing module; the need for beam splitting and wavelength adjustment of a single wavelength laser beam outputted by a laser is thereby avoided.

A system for multimodal nonlinear optical imaging is provided. Each mode uses a high NA objective to cause total internal reflection excitation at a sample-substrate interface. The system has a femtosecond oscillator to generate pulses used for two beams. The objective receives at least one beam, redirects the received at least one beam through a dielectric substrate to cause the TIR and produces corresponding evanescent waves in a portion of the sample adjacent to the sample-substrate interface, and collects a backward-propagating beam of pulses of responsive light. The portion of the sample illuminated by the evanescent waves emits responsive light. Different modes or combinations of the distinct modalities may be selected to access complementary chemical and structural information for various chemical species near the sample-substrate interface. Each mode may have mode-specific control such as selective beam blocking, power ratios and filtering.

A Raman spectrometric imaging method, including: placing a sample on a two-dimensional translation stage; emitting a first light beam by a first optical comb light source; dividing the first light beam into a pump light beam and a depletion light beam to illuminate the sample; guiding the pump light beam to illuminate a region of the sample to excite molecules of the sample in the region; guiding the depletion light beam to the region of the sample to make excited molecules at a periphery of the region to return into a vibrational ground state; emitting a second light beam as a probe light beam by a second optical comb light source to the remaining excited molecules to generate a CARS signal; recording the CARS signal for imaging; moving the two-dimensional translation stage to scan other regions of the sample to form an image of the sample.

The present invention provides for a novel series of accessories for Raman and/or luminescence spectral acquisitions for many different applications and methods for making such accessories. The invention further provides sample holders that enhance sample handling ability and sample sensitivity, reduce fluorescence and Raman background, as well as sample size and consumption, and thereby improve resulting spectral analyses.

An apparatus to provide a label-free or native particle analysis comprises a light generating system producing first light pulses at a first wavelength and second light pulses at a second wavelength; and a flow cell coupled to the light generating system to convey particles for analysis. The light generating system is configured to chirp at least one of the first light pulses and the second light pulses to analyze the particles.

Systems and methods for operating a dual-comb laser. The methods comprise: generating pulsed laser beams by first and second laser sources of the dual-comb laser, at least one of the first and second laser sources comprises a diode pumped solid state laser with an output intensity that is modifiable; and matching phase repetition rates of the pulsed laser beams by selectively modifying the output intensity of the diode pumped solid state laser.

A system includes an optical module for supplying a Stokes light, a pump light and a probe light for generating a CARS light is provided. The optical module includes a fiber laser module and an optical plate. The fiber laser module includes an oscillator, a generator, a first amplifier, a second amplifier and a LD power distributor that is configured to distribute a laser power from a first laser diode to the oscillator as an oscillation source, to the generator as a pump power, to the first preamplifier as a pump power and to the second preamplifier as a pump power.