A micro-colorimetric sensor for sensing target chemicals using edge tracking includes a substrate. A plurality of parallel linear channels of porous media is entrenched into the substrate and each linear channel includes a sensing material adapted to sense one of several specific target chemicals in air. The plurality of parallel linear channels is separated by barrier material from the adjacent parallel linear channel where the barrier material blocks diffusion of chemicals from one linear channel to another. A plate is affixed over the substrate top to cover the plurality of parallel linear channels. An air sample is diffused along the micro-colorimetric sensor and color images are captured. An intensity profile is derived from the plurality of color images to determine a maximum and a minimum intensity value along the sensor. A plurality of positions along the sensor is tracked to determine an edge position.

A micro-colorimetric sensor for sensing target chemicals that converts time sequence information into a spatial distribution of color. By tracking the spatial color distribution, chemical exposure over time is thus detected, which overcomes the limitation of traditional colorimetric sensors. A porous media is coated on a top surface of the substrate. Multiple sensing chemicals are fused in parallel linear channels into the porous media coating. A plate is affixed over the substrate top surface to cover the plurality of parallel linear channels. An air sample is diffused along the porous media to get a clear pattern of spatial color distribution and color images are captured. Optical parameters like gradient of spatial color distribution, intensity, and absorbance, etc., can be tracked to calculate analytes concentrations.

A plasmon resonance system, instrument, cartridge, and methods for analysis of analytes is disclosed. A PR system is provided that may include a DMF-LSPR cartridge that may support both digital microfluidic (DMF) capability and localized surface plasmon resonance (LSPR) capability for analysis of analytes. In some examples, the DMF portion of the DMF-LSPR cartridge may include an electrode arrangement for performing droplet operations, whereas the LSPR portion of the DMF-LSPR cartridge may include an LSPR sensor. In other examples, the LSPR portion of the DMF-LSPR cartridge may include an in-line reference channel, wherein the in-line reference channel may be a fluid channel including at least one functionalized LSPR sensor (or sample spot) and at least one non-functionalized LSPR sensor (or reference spot). Additionally, methods of using the PR system for analysis of analytes are provided.

A lateral flow assay device includes several markers, a color bar, and/or a grayscale on the housing of the lateral flow assay device. The markers are used to assist in focusing a mobile device's camera on the control line of the lateral flow assay device. The markers may be used to adjust the perspective of an image taken from the control line and the test line of the lateral flow assay device. The markers may be used to locate the images of the control line, the test line, the color bar, and/or the grayscale on the image. The image of the color bar and the grayscale may be used to adjust the colors and intensity of the image. The images of the test line and the control line may then be used to determine the test results of the lateral flow assay device.

In one example, a flow cell includes a base support and a protrusion over the base support, where the protrusion is a different material than the base support. The flow cell further includes a first functionalized layer over a first portion of the protrusion, a second functionalized layer over a second portion of the protrusion, and first and second primer sets respectively attached to the first and second functionalized layers.

An example flow cell includes a multi-layer stack including a transparent base support; a patterned sacrificial layer over the transparent base support; and a transparent layer over the patterned sacrificial layer. The flow cell further includes first and second functionalized layers over different portions of the transparent layer, wherein at least one of the first and second functionalized layers aligns with a pattern of the patterned sacrificial layer; and first and second primer sets respectively attached to the first and second functionalized layer.

An optical waveguide interferometer cartridge system and related methods are provided. The optical waveguide interferometer cartridge system includes a cartridge housing comprising an interferometric chip and a flow cell wafer as well as an alignment means for aligning the cartridge system within the interferometric system.

A point of use analyte detection and quantification system for agricultural applications is provided. Related methods are also provided.

A point of use analyte detection and quantification system for aquatic applications is provided. Related methods are also provided.

Methods of detecting one or more viral analytes with an optical interferometric system is provided. Methods of diagnosing a viral infection in a patient with an optical interferometric system are also provided.