Disclosed is a method for visually identifying quality of Lilii Bulbus, including: uniformly mixing an extracting solution of a Lilii Bulbus sample to be detected with gold-silver nanoclusters, standing the mixed extracting solution for reaction, and realizing visual identification of Lilii Bulbus quality according to information of color or information of fluorescence intensity of a detection system obtained after the reaction and response difference of the color or fluorescence of Lilii Bulbus samples with different quality. Compared with that existing detection method based on chromatography and the like, the method provided by the present application has the advantages of high stability, fast response, easy operation and portable devices in addition to a broad application prospect for industrial production.

A detection device according to an aspect of the present disclosure includes a detection portion that contains a substance that emits fluorescence in a case of being irradiated with light when reacting with a plant hormone.

An optofluidic diagnostic system and methods for rapid analyte detections. The system comprises an optofluidic sensor array, a test plate and an optical detection cartridge. The sensor array supports one or more distinct sensor units, each having a reactor section designed to temporarily enter a series of different kinds of wells in the test plate. One kind of well is a sample reservoir that holds reagent solution to be transferred into the reactor section. Another kind of well is a drainage chamber that removes reagent solution from the reactor section. A third kind of well is a colorant reservoir that holds a colorant reagent transferable into a reactor section. Finally, the sensor unit is transferred to the optical detection cartridge where it is placed into an isolation booth during the optical detection process so that its flat observation face is stationed in a viewing window opposite an optical detector lens.

The present invention relates to a method and a device for quantitatively detecting the presence or absence of an analyte in a liquid sample, comprising the steps of providing a set of parts comprising a container for collecting a liquid sample material, and a filter material and a detection device comprising a reaction liquid, thereafter adding a metered amount of liquid sample material to the container, thereafter transferring the metered amount of liquid sample material from the container to the filter material, thereafter contacting the filter material containing the metered amount of sample material with the reaction liquid and mixing the reaction liquid and the filter material, thereby obtaining a detection liquid, thereafter measuring the transmission of electromagnetic radiation at one or more wavelengths through the detection liquid and/or the emission of electromagnetic radiation at one or more wavelengths from the detection liquid and detecting the amount of analyte in the sample by comparing the results obtained in step e. with an internal standard, the method being characterised in that the metered amount of sample is transferred from the container to the filter material by use of capillary forces.

A colorimetric sensor has a first material deposited on a surface, and sensing particles on a surface of the first material, wherein the sensing particles comprise sensing species dispersed into porous host structures, such that at least a portion of the sensing particles is exposed to an ambient environment, wherein the first material attaches the sensing particles to surface. A method of forming a colorimetric sensor including depositing a first material onto a substrate, providing porous sensing particles, wherein the sensing particles comprise sensing species dispersed into a porous host structure, and embedding the porous sensing particles onto a surface of the deposited first material, wherein the first material attaches the sensing particles to the substrate such that at least a portion of the sensing particles is exposed to an ambient environment.

The present disclosure relates to an electrochemical sensor for determining a measurand correlating with a concentration of an analyte in a measuring fluid, comprising: a sensor membrane designed to be in contact with the measuring fluid for detecting measured values of the measurand; a probe housing which has at least one immersion region designed for immersion into the measuring fluid, wherein the sensor membrane is arranged in the immersion region of the probe housing; and a measurement circuit which is at least partially contained in the probe housing and is designed to generate and output a measurement signal dependent on the measurand, wherein the sensor membrane contains an optically detectable substance for marking the sensor membrane.

A device configured to provide a value indicative of fluorescent emission from a substrate 5 having a test region is provided. The device comprises an electromagnetic radiation source configured to emit excitation radiation towards the test region to excite fluorescent emission from a fluorescent material in the test region. The electromagnetic radiation source is configured such that a variation in intensity of the excitation radiation across the test region is less than 15%. The device further comprises a sensor configured to capture a primary 10 image of the fluorescent emission, and a controller configured to modify the primary image based on calibration data and to use the modified image to obtain the value indicative of the fluorescent emission.

The disclosed technology brings histopathology into the operating theatre, to enable real-time intra-operative digital pathology. The disclosed technology utilizes confocal imaging devices image, in the operating theatre, “optical slices” of fresh tissue—without the need to physically slice and otherwise process the resected tissue as required by frozen section analysis (FSA). The disclosed technology, in certain embodiments, includes a simple, operating-table-side digital histology scanner, with the capability of rapidly scanning all outer margins of a tissue sample (e.g., resection lump, removed tissue mass). Using point-scanning microscopy technology, the disclosed technology, in certain embodiments, precisely scans a thin “optical section” of the resected tissue, and sends the digital image to a pathologist rather than the real tissue, thereby providing the pathologist with the opportunity to analyze the tissue intra-operatively. Thus, the disclosed technology provides digital images with similar information content as FSA, but faster and without destroying the tissue sample itself.

Described herein is a method of preparing a bifunctional assay surface. In particular, a method is provided for preparing an assay surface that includes a primary reagent and a secondary reagent. In one aspect, the primary and secondary reagents are immobilized on the assay surface by different surface chemistries. In one aspect, a method is provided for preparing an assay surface that includes a proteinaceous primary reagent and a thiol-containing secondary reagent. In one aspect, a method is provided for preparing an assay surface that includes a capture-target hybrid and a thiol-containing secondary reagent.

The compounds relate to zinc-sensitive fluorescent probes, compositions and methods utilizing the same. Such compounds provide an emission-ratiometric fluorescence response upon binding of an analyte. In some embodiments, compounds can be used for two-photon excitation microscopy or conventional fluorescence microscopy. The compounds described herein can also contain one or more functional groups to improve the emission-ratiometric fluorescence response.