A blood analyzing method includes optically measuring a first calibration sample prepared from a fibrin/fibrinogen degradation product (FDP) measurement reagent and a first calibrator containing D-dimer (DD) and having a first value relating to the ratio of the content of FDP to the content of DD, acquiring first calculation data based on temporal change of optical information of the first calibration measurement sample, performing optical measurement of a second calibration measurement sample prepared from FDP measurement reagent and a second calibrator containing DD and having a second value that is different from the first value, acquiring second calculated data based on a temporal change in optical information of the second calibration measurement sample, and acquiring calibration curve information indicating the relationship between the calculation data and the value relating to the amount of DD.

Systems and methods for imaging and tracking fibrin formation via interaction of a test sample with a clotting agent or for imaging and tracking fibrin removal by an anti-clotting agent are described.In certain embodiments, the systems (200) comprise a planar reflective substrate (222, 224) comprising one or more capture agents and/or one or more fibrin reference regions; a mount for holding the substrate; an illumination light source (201) for directing illumination light toward a top surface of the substrate with fibrin (226)formed thereon; an image detector (232, 234) aligned with respect to the mount for detecting a portion of the illumination light that is scattered by the fibrin, and/or reflected by the reflective substrate, thereby obtaining a label-free image of fibrin formation or fibrin removal; a processor of a computing device (240); and a memory having instructions stored thereon, wherein the instructions, when executed by the processor, cause the processor to: receive and/or access data corresponding to the one or more label free images, and use the one or more label-free images to determine one or more measures of fibrin formation or fibrin removal.

A sample measurement method of performing first measurement for a blood coagulation test and second measurement for a test different from the blood coagulation test includes: dispensing a sample for use in the first measurement into a first container from a sample container; dispensing the sample for use in the second measurement into a second container different from the first container from the sample container from which the sample for use in the first measurement has been dispensed; performing the first measurement based on the sample dispensed into the first container; and performing the second measurement based on the sample dispensed into the second container.

Provided are an analysis device, an analysis method, and the like that predict a concentration even for a sample having high concentration that can be outside a measurement range as it is, using an absorbance measurement result in a reaction course at the time of measurement of a test liquid containing an immunological reagent and the sample, and determine an optimum dilution rate to conduct measurement. As the present invention, for example, an analysis device having means (A) that detects a prozone during measurement of a sample, and means (B) that determines a high concentration region by automatically determining a dilution rate of the test liquid can be recited.

Protecting information by partitioning data across multiple storage resources. A method includes receiving an image of a test card and an identity of a patient associated with the test card. The method includes associating the image of the test card with the identity of the patient as a data pair on a first storage resource. The method includes providing the image of the test card to a second storage resource by way of a network, wherein the first storage resource is independent of the second storage resource. The method includes receiving a result for the test card and associating the result for the test card with the identity of the patient based on the data pair stored on the first storage resource.

Test cards for agglutination assays, wherein the test cards are configured for computer-implemented image analysis. A test card includes a negative control test region for receiving a negative control fluid sample, a positive control test region for receiving a positive control fluid sample, and a test sample region for receiving a test fluid sample. The test card includes one or more unique scannable codes comprising data for instructing a processor to capture an image of the test card that is suitable for computer-implemented image analysis.

Machine learning image analysis for quantitative and qualitative analysis of agglutination samples. A method includes receiving an image of an agglutination assay comprising a negative control sample, a positive control sample, and a test sample. The method includes providing the image to a machine learning algorithm trained to classify agglutination of the test sample on a quantitative scale. The machine learning algorithm calibrates the quantitative scale based at least in part on the negative control sample and the positive control sample.

A testing device for identifying an antigen or antibody within a biofluid sample including: a substrate having a hydrophilic surface thereon; the surface including a collection zone, and at least one detection zone extending therefrom; wherein the biofluid sample can be mixed with a specific antigen or antibody, and deposited on the collection zone and transferred by capillary action to the detection zone; the antigen or antibody in the biofluid sample reacting with an appropriate said antibody or antigen thereby resulting in a visual indication within the detection zone.

Method for detecting the presence or absence of a biological or chemical substance in a particular sample mixed with a suspension with functionalized magnetic particles, comprising: providing a light source and detector, providing a constant magnetic force perpendicular to the light's propagation direction by applying a constant magnetic field gradient, and with an absolute value which is higher than 0.1 T and measuring the change of the magnetic particle's suspension transparency versus time and comparing it with the time-variation in absence of the targeted biological or chemical substance. The method of the invention allows monitoring the transparency irrespective of the emitted wavelength and particle's optical properties.

Methods, devices, and systems for analyte analysis using a nanopore are disclosed. The methods, devices, and systems utilize a first and a second binding member that each specifically bind to an analyte in a biological sample. The method further includes detecting and/or counting a cleavable tag attached to the second binding member and correlating the presence and/or the number of tags to presence and/or concentration of the analyte. Certain aspects of the methods do not involve a tag, rather the second binding member may be directly detected/quantitated. The detecting and/or counting may be performed by translocating the tag/second binding member through a nanopore. Devices and systems that are programmed to carry out the disclosed methods are also provided. Also provided herein are instruments that are programmed to operate a cartridge that includes an array of electrodes for actuating a droplet and further includes an electrochemical species sensing region. The instrument may be used to analyse a sample in a cartridge that includes an array of electrodes for actuating a droplet and further includes a nanopore layer for detecting translocation of a tag/second binding member through nanopore. An instrument configured to operate a first cartridge that includes an array of electrodes for actuating a droplet and further includes an electrochemical species sensing region and a second cartridge that includes an array of electrodes for actuating a droplet and further includes a nanopore layer for detecting translocation of a tag/second binding member through nanopore is disclosed. An instrument configured to operate a cartridge that includes an array of electrodes for actuating a droplet, an electrochemical species sensing region, and a nanopore layer for detecting translocation of a tag/second binding member through nanopore is disclosed.