The present invention relates to an analysis system that is applied to rapid automatic analysis and detection of highly lipophilic components and a method for rapidly detecting vitamin D in an oil or fat, or a biological sample. In the present invention, online pretreatment and separation of a sample containing highly lipophilic components in a sample to be analyzed are performed by a multidimensional chromatography system. The multidimensional chromatography system includes a supercritical chromatography part and a reverse phase liquid chromatography part sequentially connected, the reverse phase liquid chromatography part including one or more reverse phase liquid chromatography columns, and the supercritical chromatography part including a supercritical mobile phase, a modifier, and a supercritical packed column.

Disclosed is a proteomics sample preparation device, comprising a pipette tip, an ion exchange resin filler and a solid-phase extraction membrane. The solid-phase extraction membrane is filled into the lower end of the pipette tip, and the ion exchange resin filler is filled into the lower end of the pipette tip and is located above the solid-phase extraction membrane. The ion exchange resin filler is a strong cation exchange resin filler or a strong anion exchange resin filler. Disclosed is a protein chromatographic separation platform comprising the proteomic reactor and a liquid chromatography-mass spectrometer. Disclosed is the use of the proteomics reactor and protein chromatographic separation platform in the protein identification and protein quantitative analysis of a cell, a tissue or a blood sample.

A preparative liquid chromatograph that separates components in a sample in a separating column and captures a plurality of target components in an eluate from the separating column in individual trap columns, includes: a column switching means configured to switch passages to cause the eluate having been eluted from the separating column and passed through a detector to selectively flow into one of the trap columns, a passage switching means disposed in a passage between the detector and the column switching means and configured to switch between a first state in which the eluate flows to the column switching means and a second state in which the eluate is discharged without flowing to the column switching means. For the switching among the trap columns, the passage switching means and the column switching means are controlled to set the passage switching means in the second state (step S17) before performing the switching operation of the column switching means (step S18). This prevents entry of a target component into a wrong trap column during the switching among the trap columns.

Disclosed are a composition and system for separating and detecting an alpha-fetoprotein variant, comprising a separation reagent and a detection reagent; a system for separating and detecting an alpha-fetoprotein variant and a use thereof; and a kit for separating and detecting the alpha-fetoprotein variant. By means of the composition and system for separating and detecting the alpha-fetoprotein variant, and the use thereof, primary liver cancer can be indicated early on, the sensitivity is high, and the method is rapid, simple and automated.

Systems and methods are provided for determining a content of a hydrocarbon or other compound, such as a C.sub.3 to C.sub.7 hydrocarbon, in a condensed steam sample. Cooled steam condensate can be flowed through a sample chamber including an inner overflow tube. When the flow stops, water can be drained from the sample chamber, and then the sample chamber can be opened to allow fluid communication with a vapor chamber above the sample chamber. This can allow hydrocarbons in the condensed steam (and/or other gas) to be transferred from the sample chamber into the vapor chamber. The vapor chamber can then be isolated from the sample chamber. At least a portion of the content of the vapor chamber can then be passed to a detection volume, such as the characterization cell for a gas chromatography system.

Disclosed is a proteomic reactor, comprising a pipette tip, an ion exchange resin filler and a solid-phase extraction membrane. The solid-phase extraction membrane is filled into the lower end of the pipette tip, and the ion exchange resin is filled into the lower end of the pipette tip and is located above the solid-phase extraction membrane. The ion exchange resin is a strong cation exchange resin or a strong anion exchange resin. Disclosed is a protein chromatographic separation platform comprising the proteomic reactor and a liquid chromatography-mass spectrometer. Disclosed is the use of the proteomic reactor and protein chromatographic separation platform in the protein identification and protein quantitative analysis of a cell, a tissue or a blood sample.

A method for determining corrosion inhibitor residual concentration of a hydrocarbon sample is described. The hydrocarbon sample is mixed with a standard solution to form a first mixture. The standard solution includes a corrosion inhibitor in a known concentration. The first mixture is mixed with an aqueous saline solution to form a second mixture. The aqueous saline solution includes about 1% salt concentration or greater. The second mixture is agitated for about 1 hour or longer and at a temperature of about 50 degrees Celsius (? C.) or greater. After agitation, a hydrocarbon phase and an aqueous phase of the second mixture are allowed to separate. A portion of the aqueous phase is obtained. The portion of the aqueous phase is analyzed to determine a corrosion inhibitor residual concentration of the hydrocarbon sample.

A method for extracting an analyte in a sample is described. A sample and a solution in a microwave-extraction vial are microwave-heated and agitated. A vapor produced in the vial can be extracted into a liquid-phase medium contained in a porous membrane bag situated in the vial. The liquid-phase medium containing the vapor extract may then be analyzed for an analyte with gas chromatography-mass spectrometry.

A method for extracting an analyte in a sample is described. A sample and a solution in a microwave-extraction vial are microwave-heated and agitated. A vapor produced in the vial can be extracted into a liquid-phase medium contained in a porous membrane bag situated in the vial. The liquid-phase medium containing the vapor extract may then be analyzed for an analyte with gas chromatography-mass spectrometry.

The present invention refers to a method of purifying a glucagon-like peptide 1 analogs, the method comprising a two dimensional reversed phase high performance liquid chromatography protocol, wherein the first step is carried out at a pH value between 7.0 to 7.8 using a mobile phase comprising a phosphate buffer and acetonitrile, and the second step is carried out at a pH value below 3.0 using a mobile phase comprising trifluoroacetic acid and acetonitrile.