A method for isolated a protein complex comprising BCL10 and at least one, preferably all, of ROS1, LSD1, BTK, KU80, KU70, CUL4A, IMP3, thioredoxin, hTID1, DAP3, CDK1/CDC2, PRL1/PTP4A1 or NM23. Methods for using this complex to diagnose or prognose diseases including diabetes, obesity, cancer, neurodegenerative disease or inflammatory diseases associated with activation of NF-κB Methods for distinguishing lean, obese and diabetic subjects based on expression of BCL10 and its ligands are also disclosed. The invention also pertains to pharmaceutical compositions comprising ligands for BCL10 or other components of this complex or agents such as siRNA or miRNA that regulate the expression of the protein components of this complex.

The present invention is a method for detecting a specific disease based on the result of a measurement in which the amount of a peptide serving as a biomarker contained in a biological sample is determined by using an LC-MS. A pretreatment process performed before the measurement using the LC-MS includes the steps of preparing a mixed sample solution by adding a stable isotope reagent and a trifluoroacetic acid to the biological sample, where the stable isotope reagent is prepared beforehand by labeling the peptide with a stable isotope; boiling the mixed sample solution; injecting the mixed sample solution after boiled into a solid-phase extraction column to make the peptide be retained in the solid-phase extraction column; and passing a water-soluble organic solvent through the solid-phase extraction column to elute the peptide retained in the solid-phase extraction column and collect the eluate.

Provided is a method for analyzing a diene compound including: a triazolinedione adduct heating step of heating a triazolinedione adduct to produce a triazolinedione compound; an ene compound formation step of reacting the triazolinedione compound with a diene compound to obtain an ene compound; and an ene compound analysis step of analyzing the ene compound to quantitative determine the diene compound. Also provided is a method for producing an ene compound, including: a triazolinedione adduct heating step of heating a triazolinedione adduct to produce a triazolinedione compound; and an ene compound formation step of reacting the triazolinedione compound with a diene compound to obtain an ene compound.

A method for quantitative detection of trace polylactic acid microplastics (PLA MPs) in an environmental sample is disclosed, where the PLA MP in the environmental sample is depolymerized in an alkaline alcohol phase system, and the monomers thereof, i.e., lactic acid molecules were separated and recovered. Mass concentrations of lactic acid before and after reaction are quantitatively detected by HPLC-MS, and the initial mass concentration of PLA MPs in the sample is calculated using a formula. The minimum detectable concentration may be as low as 0.05 mg/kg. This method can quantify the trace PLA in water body, sludge, sediment, dust, and soil samples; no special treatment is required before heating in pentanol; use of such reaction conditions as heating and alkaline alcohol phase reduces reaction time substantially.

Disclosed is a method of: providing a fiber having propylene oxide adsorbed thereon; exposing the fiber to a gaseous sample; allowing the propylene oxide to react with any chlorine in the sample to form chloro-2-propanol. The method can be used to detect potassium chlorate.

A method for efficiently purifying and detecting 6-hydroxynobilonine in fresh stems of dendrobium huoshanense uses a gas chromatography-mass spectrometry (GC-MS) method to detect 6-hydroxynobilonine in dendrobium huoshanense is, i.e., a to-be-detected solution of 6-hydroxynobilonine is extracted from fresh stems of dendrobium huoshanense, the extraction method is as follows: freeze-drying, smashing and screening fresh stems of dendrobium huoshanense to obtain dendrobium dry powder, adding water for performing ultrasonic treatment, and then adding a composite enzyme for enzymolysis to obtain an enzymolysis solution; adding acidity alcohol into the enzymolysis solution, extracting for 1 min under an ultra-high pressure, and then taking filtrate; and concentrating the filtrate in vacuum, then purifying using a mixed-mode cation exchanger (MCX) extraction column, eluting with a methanol-acetonitrile solution, collecting eluent, blowing with nitrogen until no water, and then dissolving with methanol and filtering to obtain the to-be-detected solution.

A method and kit for detecting at least two vitamin D metabolites in a biological sample is disclosed, which comprises processing the biological sample to prepare the sample for LC-MS/MS analysis, passing the prepared sample through a liquid chromatography column having an outlet connected to an inlet port of a tandem mass spectrometer to separate said two vitamin D metabolites, if present in the sample, and introduce the two vitamin D metabolites into the tandem mass spectrometer. The method further comprises generating [M+H].sup.+ ions of each of the two vitamin D metabolites in said tandem mass spectrometer, and generating two fragment ions of said [M+H].sup.+ ions associated with said vitamin D metabolites, wherein said fragment ions are not due to water losses from the [M+H].sup.+ ions; and detecting the fragment ions to identify presence of the two metabolites in the biological sample.

A liquid leak detection device is used in an oven. The oven has an oven casing having a sealed inner space provided with a sample flow path through which a liquid sample flows and a heater provided in the inner space that heats the inner space. The liquid leak detection device includes a gas sensor that is provided in the inner space and detects a liquid sample that has leaked from the sample flow path to the inner space and has been evaporated. The liquid leak detection device further includes a liquid leak pipe path that communicates with the inner space and leads a non-vaporized liquid sample that has leaked from the sample flow path to the inner space out of the oven casing, and a liquid sensor that is provided outside of the oven casing and detects a liquid sample led out by the liquid leak pipe path.

At least one stable isotope reagent is added to each biological sample and standard sample to prepare biological samples for analysis and standard sample for analysis. The quality of the biological samples is evaluated using data of one set of biological samples for analysis composed of a plurality of biological samples for analysis. Besides, the quality of a pretreatment and/or analysis of each set of samples for analysis is evaluated using data obtained by analyzing the standard sample for analysis before and after an analysis of one set of samples for analysis. An abnormality in a chromatograph or mass analyzer used for the analysis of one set of samples is evaluated by the data obtained by analyzing a sample for device evaluation before and after the analysis of one set of samples for analysis. Thus, the quality of data obtained by chromatographic mass spectrometry on biological samples is comprehensively evaluated.

The present invention relates to a dianhydride analysis method. The method can stably analyze a dianhydride which has high reactivity and low solubility. Furthermore, the method can minimize the problem that a reaction product disturbs an analysis result, thereby improving accuracy of the analysis result.