A hydrocarbon generation pyrolysis simulation experimental device for centrifugal continuous gas sampling of a hydrocarbon source rock, including a centrifugal turntable, a motor, a quartz sample tube, a heating set, a cooling set, a rotary joint mounted coaxially with a rotating shaft of the centrifugal turntable, a vacuum pump, and vacuum gas collecting pipes, wherein a sealing plug is arranged at an orifice of the quartz sample tube, a thermocouple and a first exhaust pipeline connected with an inlet of the rotary joint are mounted on the sealing plug, the rotary joint is communicated with a vacuum pump through a second exhaust pipeline, a plurality of vacuum gas collecting pipes are respectively communicated with the second exhaust pipeline through an electromagnetic valve, a vacuum pump switching valve is mounted on the second exhaust pipeline at an inlet end of the vacuum pump, and a control circuit board is mounted on the centrifugal turntable.

The invention relates to a method for the treatment of body fluid proteins, by which proteins from body fluids such as blood or urine are extracted by adding a certain proportion of high molecular polymer solution under low temperature condition followed by denaturation and reduction by adding a certain concentration of surfactant and tris(2-carboxyethyl) phosphine (TCEP) under a high temperature condition. Subsequently, the iodoacetic acid brushes grafted on silica microspheres called as solid-phase alkylation reagents are added into protein solution, which can react rapidly with the protein sulfhydryl group. After centrifugation, the microspheres are obtained and repeatedly washed with methanol and buffer to remove interferences such as sugars, salts, surfactants, lipids to obtain high-purity proteins, and finally protease is added to digest proteins into peptides. After centrifugation, the peptide products are obtained, and directly analyzed by liquid chromatography-mass spectrometry (LC-MS) system. Compared with the traditional protein pretreatment method, the method has many advantages such as good anti-interference capability, easy operation and short pretreatment time.

The invention relates to a method for the treatment of body fluid proteins, by which proteins from body fluids such as blood or urine are extracted by adding a certain proportion of high molecular polymer solution under low temperature condition followed by denaturation and reduction by adding a certain concentration of surfactant and tris(2-carboxyethyl) phosphine (TCEP) under a high temperature condition. Subsequently, the iodoacetic acid brushes grafted on silica microspheres called as solid-phase alkylation reagents are added into protein solution, which can react rapidly with the protein sulfhydryl group. After centrifugation, the microspheres are obtained and repeatedly washed with methanol and buffer to remove interferences such as sugars, salts, surfactants, lipids to obtain high-purity proteins, and finally protease is added to digest proteins into peptides. After centrifugation, the peptide products are obtained, and directly analyzed by liquid chromatography-mass spectrometry (LC-MS) system. Compared with the traditional protein pretreatment method, the method has many advantages such as good anti-interference capability, easy operation and short pretreatment time.

A separation device is instructed to separate a compound from a sample over a time period. A mass spectrometer is instructed to measure a plurality of intensities of at least one ion of the separated compound over the time period, producing a chromatogram. At least one peak of the at least one ion is identified from the chromatogram using a peak-finding algorithm. Two or more different peak integration areas are calculated for the at least one peak by applying the peak-finding algorithm with two or more different values for at least one peak-finding parameter. Two or more plots of the at least one peak that each shows graphically a different peak integration area are displayed on a display device at the same time. In response, data is received from a user selection device that indicates user selection of one of the two or more plots.

A separation device is instructed to separate a compound from a sample over a time period. A mass spectrometer is instructed to measure a plurality of intensities of at least one ion of the separated compound over the time period, producing a chromatogram. At least one peak of the at least one ion is identified from the chromatogram using a peak-finding algorithm. Two or more different peak integration areas are calculated for the at least one peak by applying the peak-finding algorithm with two or more different values for at least one peak-finding parameter. Two or more plots of the at least one peak that each shows graphically a different peak integration area are displayed on a display device at the same time. In response, data is received from a user selection device that indicates user selection of one of the two or more plots.

Disclosed herein are methods, systems, and kits for determining the total amount of a coating polymer in a dosage form. The coating polymer may be a polydisperse polymer having a plurality of molecular weights. The instant disclosure enables to accurately, reliably, and efficiently determine the total amount of a polydisperse polymer in a dosage form that includes one or more other constituents that have a molecular weight range that overlap with at least some of the polydisperse polymer's molecular weight.

Disclosed herein are methods, systems, and kits for determining the total amount of a coating polymer in a dosage form. The coating polymer may be a polydisperse polymer having a plurality of molecular weights. The instant disclosure enables to accurately, reliably, and efficiently determine the total amount of a polydisperse polymer in a dosage form that includes one or more other constituents that have a molecular weight range that overlap with at least some of the polydisperse polymer's molecular weight.

In a preparative liquid chromatograph apparatus where a sample containing compounds temporally separated from each other by LC is introduced into a detector and a fraction collector to collect fractions of the sample each containing one or more compounds. A result storage section stores analysis results obtained for a plurality of samples by performing, for each identical sample, LC analyses under different separation conditions. A display-target selection receiver displays an analysis result display screen having a plurality of result display areas for individually displaying results of different LC analyses and a selection indication area for allowing a user operation for selecting one sample from a plurality of samples, and receives the user operation in the selection indication area. An analysis result display processor retrieves, from the result storage section, a plurality of analysis results corresponding to the selected sample, and displays the analysis results in the result display areas.

In a preparative liquid chromatograph apparatus where a sample containing compounds temporally separated from each other by LC is introduced into a detector and a fraction collector to collect fractions of the sample each containing one or more compounds. A result storage section stores analysis results obtained for a plurality of samples by performing, for each identical sample, LC analyses under different separation conditions. A display-target selection receiver displays an analysis result display screen having a plurality of result display areas for individually displaying results of different LC analyses and a selection indication area for allowing a user operation for selecting one sample from a plurality of samples, and receives the user operation in the selection indication area. An analysis result display processor retrieves, from the result storage section, a plurality of analysis results corresponding to the selected sample, and displays the analysis results in the result display areas.

Packed-tip electrospray ionization (ESI) emitters for mass spectrometry are described. In one aspect an ESI emitter stores a first type of particle. A liquid chromatography (LC) column is coupled with the emitter via a junction. The LC column stores a different type of particle than the ESI emitter to facilitate better chromatographic and ESI performance.