A controller (50) of a liquid chromatograph (1) is configured to execute, as an analysis operation in an analysis unit (3), a sample injection step of bringing a high-pressure valve (10) into a loading state, sucking a sample from a tip of a needle (12) to hold a sample in a sampling channel (2), then connecting the sampling channel (2) to an injection port (16) and bringing the high-pressure valve (10) into an injecting state, and supplying a mobile phase from a liquid supplier (6), thereby injecting a sample held in the sampling channel (12) into an analysis channel (4), and an analysis step of separating components of a sample injected into the analysis channel (4) in a separation column (14) by bringing the high-pressure valve (10) in the loading state and supplying the mobile phase from the liquid supplier (6) after the sample injection step is ended. In a case where at least a predetermined condition is satisfied, after the analysis step is ended, the controller is configured to execute, as the analysis operation, a system cleaning step of cleaning a liquid flowing route from the sampling channel (2) to the analysis channel (4) by connecting the sampling channel (2) to the injection port (16) and bringing the high-pressure valve (10) into the injecting state, and supplying the mobile phase and/or a cleaning liquid from the liquid supplier (6).

A controller (50) of a liquid chromatograph (1) is configured to execute, as an analysis operation in an analysis unit (3), a sample injection step of bringing a high-pressure valve (10) into a loading state, sucking a sample from a tip of a needle (12) to hold a sample in a sampling channel (2), then connecting the sampling channel (2) to an injection port (16) and bringing the high-pressure valve (10) into an injecting state, and supplying a mobile phase from a liquid supplier (6), thereby injecting a sample held in the sampling channel (12) into an analysis channel (4), and an analysis step of separating components of a sample injected into the analysis channel (4) in a separation column (14) by bringing the high-pressure valve (10) in the loading state and supplying the mobile phase from the liquid supplier (6) after the sample injection step is ended. In a case where at least a predetermined condition is satisfied, after the analysis step is ended, the controller is configured to execute, as the analysis operation, a system cleaning step of cleaning a liquid flowing route from the sampling channel (2) to the analysis channel (4) by connecting the sampling channel (2) to the injection port (16) and bringing the high-pressure valve (10) into the injecting state, and supplying the mobile phase and/or a cleaning liquid from the liquid supplier (6).

Examples of the present disclosure describe systems and methods for detecting and mitigating stack pivoting exploits. In aspects, various “checkpoints” may be identified in software code. At each checkpoint, the current stack pointer, stack base, and stack limit for each mode of execution may be obtained. The current stack pointer for each mode of execution may be evaluated to determine whether the stack pointer falls within a stack range between the stack base and the stack limit of the respective mode of execution. When the stack pointer is determined to be outside of the expected stack range, a stack pivot exploit is detected and one or more remedial actions may be automatically performed.

Examples of the present disclosure describe systems and methods for detecting and mitigating stack pivoting exploits. In aspects, various “checkpoints” may be identified in software code. At each checkpoint, the current stack pointer, stack base, and stack limit for each mode of execution may be obtained. The current stack pointer for each mode of execution may be evaluated to determine whether the stack pointer falls within a stack range between the stack base and the stack limit of the respective mode of execution. When the stack pointer is determined to be outside of the expected stack range, a stack pivot exploit is detected and one or more remedial actions may be automatically performed.

A chromatography system includes a gradient delay volume defined as an overall fluid volume between where gradient is proportioned until an inlet of a chromatography column, a pump pumping a flow of gradient; and at least one valve located downstream from the pump, the at least one valve having a plurality of ports including an inlet port that receives the flow of gradient from the pump and an outlet port through which the flow of gradient exits the at least one valve, the at least one valve having at least two positions. A first position of the at least two positions of the at least one valve increases the gradient delay volume of the chromatography system relative to when the at least one valve is in a second position.

A chromatography system includes a gradient delay volume defined as an overall fluid volume between where gradient is proportioned until an inlet of a chromatography column, a pump pumping a flow of gradient; and at least one valve located downstream from the pump, the at least one valve having a plurality of ports including an inlet port that receives the flow of gradient from the pump and an outlet port through which the flow of gradient exits the at least one valve, the at least one valve having at least two positions. A first position of the at least two positions of the at least one valve increases the gradient delay volume of the chromatography system relative to when the at least one valve is in a second position.

A method performed in a liquid chromatography system includes a metering device pushing a sample into a trap column. The metering device sucks in the sample from a sample reservoir, wherein the sucking in the sample from a sample reservoir precedes the step of pushing the sample into the trap column. The liquid chromatography system also includes a trap column and a metering device, wherein the system is adapted to assume a configuration allowing the metering device to push a sample into the trap column and wherein the metering device is adapted to push the sample into the trap column in this configuration, wherein the system is adapted to assume a configuration allowing the sample to be sucked into the system by means of the metering device. Furthermore, the invention relates to a use of the liquid chromatography system for liquid chromatography, in particular of high-pressure liquid chromatography.

A method performed in a liquid chromatography system includes a metering device pushing a sample into a trap column. The metering device sucks in the sample from a sample reservoir, wherein the sucking in the sample from a sample reservoir precedes the step of pushing the sample into the trap column. The liquid chromatography system also includes a trap column and a metering device, wherein the system is adapted to assume a configuration allowing the metering device to push a sample into the trap column and wherein the metering device is adapted to push the sample into the trap column in this configuration, wherein the system is adapted to assume a configuration allowing the sample to be sucked into the system by means of the metering device. Furthermore, the invention relates to a use of the liquid chromatography system for liquid chromatography, in particular of high-pressure liquid chromatography.

A container assembly for use with a high-pressure liquid chromatography (HPLC) instrument is disclosed, in which the container assembly, when coupled to a source of pressurized gas, provides fluid medium to the HPLC instrument at positive pressure. The container assembly has an external exterior container shell, an internal fluid container for holding fluid medium, an interstitial volume between the external exterior container shell and the internal fluid container, a port for fluidly connecting the volume to a pressurized gas source, and a port for fluidly connecting the internal fluid container to the HPLC instrument. As a pressurized gas in the interstitial volume increases, fluid medium flows out of the port connected to the internal fluid bag and container assembly at a positive pressure. A system incorporating the container assembly, and method of use of the same, are also disclosed.

A container assembly for use with a high-pressure liquid chromatography (HPLC) instrument is disclosed, in which the container assembly, when coupled to a source of pressurized gas, provides fluid medium to the HPLC instrument at positive pressure. The container assembly has an external exterior container shell, an internal fluid container for holding fluid medium, an interstitial volume between the external exterior container shell and the internal fluid container, a port for fluidly connecting the volume to a pressurized gas source, and a port for fluidly connecting the internal fluid container to the HPLC instrument. As a pressurized gas in the interstitial volume increases, fluid medium flows out of the port connected to the internal fluid bag and container assembly at a positive pressure. A system incorporating the container assembly, and method of use of the same, are also disclosed.