The present application is directed to multidimensional liquid chromatography (LC) systems. In certain aspects, provided is a multidimensional LC system comprising a digestion module having a digestion column containing an immobilized proteolytic enzyme, a trapping module having a trapping column for holding the sample after digestion in the digestion module, and a separation module having a separation column for separating analytes in the sample after release of the sample from the trapping column. In certain aspects, in the direction of flow through the LC systems described herein, the modules are in the following order: digestion module; trapping module, and then separation module.

The present invention describes a method of operating a fractionation collector in chromatography, and a chromatograph system for carrying out the method, wherein a solvent composition is pumped into a chromatography column, which contains at least a portion of a first solvent that is liquid under normal conditions and contains a portion of a second solvent that is gaseous at normal conditions, and the composition flowing out of the chromatography column is at least partially introduced into a detector, wherein a composition containing the first solvent, after leaving the chromatography column, is introduced into a collection vessel of the fraction collector in dependence of the detector signal, and the amount of liquid that is introduced into a collecting vessel of the fraction collector is selected in dependence of the portion of said first solvent.

The present invention describes a method of operating a fractionation collector in chromatography, and a chromatograph system for carrying out the method, wherein a solvent composition is pumped into a chromatography column, which contains at least a portion of a first solvent that is liquid under normal conditions and contains a portion of a second solvent that is gaseous at normal conditions, and the composition flowing out of the chromatography column is at least partially introduced into a detector, wherein a composition containing the first solvent, after leaving the chromatography column, is introduced into a collection vessel of the fraction collector in dependence of the detector signal, and the amount of liquid that is introduced into a collecting vessel of the fraction collector is selected in dependence of the portion of said first solvent.

The present invention relates to a method of operating a pump generating a flow of a fluid with a pressure, the method comprising operating the pump with a pump speed S satisfying the equation S=S.sub.simple(1+COR(t)); wherein S is the pump speed, S.sub.simple is the pump speed disregarding any compression and/or expansion of the fluid, t is a time, and COR(t) is a time dependent correction function; wherein the time dependent correction function COR(t) is a product of a corrective amplitude A.sub.cor and a time-dependent function f(t), i.e., COR(t)=A.sub.cor.Math.(t); and wherein the corrective amplitude Acor is set based on a measure for the flow and a measure for the pressure. The present invention also relates to a corresponding use, a pump, a pump system and an HPLC system.

The present invention relates to a method of operating a pump generating a flow of a fluid with a pressure, the method comprising operating the pump with a pump speed S satisfying the equation S=S.sub.simple(1+COR(t)); wherein S is the pump speed, S.sub.simple is the pump speed disregarding any compression and/or expansion of the fluid, t is a time, and COR(t) is a time dependent correction function; wherein the time dependent correction function COR(t) is a product of a corrective amplitude A.sub.cor and a time-dependent function f(t), i.e., COR(t)=A.sub.cor.Math.(t); and wherein the corrective amplitude Acor is set based on a measure for the flow and a measure for the pressure. The present invention also relates to a corresponding use, a pump, a pump system and an HPLC system.

The present invention relates to a quick quantitative analysis method and analyzer for mixture concentration information. A quick quantitative analysis method for a mixture based on spectral information includes: collecting a sample of a mixture solution, and injecting into a DAD to obtain DAD data; then, calculating a spectral library by a quick qualitative analysis method based on a vector error algorithm to obtain a spectral error vector; determining a spectral curve of a component included in the data corresponding to the sample, according to a value of the error vector; and calculating a content flag value of each component in the sample and concentration information of each substance.

The present invention relates to a quick quantitative analysis method and analyzer for mixture concentration information. A quick quantitative analysis method for a mixture based on spectral information includes: collecting a sample of a mixture solution, and injecting into a DAD to obtain DAD data; then, calculating a spectral library by a quick qualitative analysis method based on a vector error algorithm to obtain a spectral error vector; determining a spectral curve of a component included in the data corresponding to the sample, according to a value of the error vector; and calculating a content flag value of each component in the sample and concentration information of each substance.

A liquid chromatography system includes a pumping system with a selector valve in fluidic communication with a pump inlet. The selector valve switches between a first position, in which the selector valve fluidically couples a solvent reservoir to the pump inlet, and a second position, in which the selector valve fluidically couples a pressurized source of liquefied carbon dioxide (for example) to the pump inlet. The liquid chromatography system can perform as a HPLC system (or as an UPLC system) when the selector valve is in the first position and as a CO.sub.2-based chromatography system when the selector valve is in the second position. The selector valve can have a third position in which both the fluidic pathway between the solvent reservoir and the pump inlet and the fluidic pathway between the pressurized source and the pump inlet are blocked. This shut-off position advantageously facilitates system maintenance.

A method for analyzing related substances in a pharmaceutical composition containing an amphiphilic block copolymer comprising a hydrophilic block and a hydrophobic block as a polymeric drug carrier, related substances identified thereby, and a method for evaluating a pharmaceutical composition by using the same are provided. Preferably, the method comprises evaluating a polymeric micelle pharmaceutical composition, which comprises an amphiphilic block copolymer comprising a hydrophilic block (A) and a hydrophobic block (B), and paclitaxel or docetaxel as a poorly water-soluble drug, by using a compound represented by Chemical Formula 1, wherein the hydrophilic block (A) comprises one or more selected from the group consisting of polyethylene glycol or derivatives thereof, polyvinylpyrrolidone, polyvinyl alcohol, polyacrylamide and combinations thereof; and the hydrophobic block (B) is polylactide.

A method for analyzing related substances in a pharmaceutical composition containing an amphiphilic block copolymer comprising a hydrophilic block and a hydrophobic block as a polymeric drug carrier, related substances identified thereby, and a method for evaluating a pharmaceutical composition by using the same are provided. Preferably, the method comprises evaluating a polymeric micelle pharmaceutical composition, which comprises an amphiphilic block copolymer comprising a hydrophilic block (A) and a hydrophobic block (B), and paclitaxel or docetaxel as a poorly water-soluble drug, by using a compound represented by Chemical Formula 1, wherein the hydrophilic block (A) comprises one or more selected from the group consisting of polyethylene glycol or derivatives thereof, polyvinylpyrrolidone, polyvinyl alcohol, polyacrylamide and combinations thereof; and the hydrophobic block (B) is polylactide.