A control device of a piston pump unit comprising at least two piston-cylinder units that operate in a phase-shifted manner for the purpose of liquid chromatography and to a piston pump unit is described. The control device corrects fluctuations of the system pressure while switching from one piston cylinder unit to the respective other piston cylinder unit. The fluctuations can occur as a result of the cooling of the liquid medium that is heated in an adiabatic manner during a pre-compression phase in the working piston. The control unit controls the piston speed of at least one piston-cylinder unit during the transition phase depending on at least one characteristic, which is ascertained from chronologically previously detected pressure values, such that variations of the system pressure as a result of the cooling of the adiabatically heated medium are at least partially compensated for.

A control device of a piston pump unit comprising at least two piston-cylinder units that operate in a phase-shifted manner for the purpose of liquid chromatography and to a piston pump unit is described. The control device corrects fluctuations of the system pressure while switching from one piston cylinder unit to the respective other piston cylinder unit. The fluctuations can occur as a result of the cooling of the liquid medium that is heated in an adiabatic manner during a pre-compression phase in the working piston. The control unit controls the piston speed of at least one piston-cylinder unit during the transition phase depending on at least one characteristic, which is ascertained from chronologically previously detected pressure values, such that variations of the system pressure as a result of the cooling of the adiabatically heated medium are at least partially compensated for.

LC techniques are disclosed. The LC technique includes providing a liquid chromatography system having a coated metallic fluid-contacting element, and transporting a fluid to contact the coated metallic fluid contacting element. Conditions for the transporting of the fluid are selected from the group consisting of the temperature of the fluid being greater than 150 degree Celsius, pressure urging the fluid being greater than 60 MPa, the fluid having a protein-containing analyte incompatible with one of titanium and polyether ether ketone, the fluid having a chelating agent incompatible with the one or both of the titanium or the polyether ether ketone.

LC techniques are disclosed. The LC technique includes providing a liquid chromatography system having a coated metallic fluid-contacting element, and transporting a fluid to contact the coated metallic fluid contacting element. Conditions for the transporting of the fluid are selected from the group consisting of the temperature of the fluid being greater than 150 degree Celsius, pressure urging the fluid being greater than 60 MPa, the fluid having a protein-containing analyte incompatible with one of titanium and polyether ether ketone, the fluid having a chelating agent incompatible with the one or both of the titanium or the polyether ether ketone.

A liquid carbon dioxide delivery pump with a pump head including a pump chamber and a refrigerant channel different from a channel passing through the pump chamber, a circulation channel for refrigerant, a refrigerant pump arranged on the circulation channel, the refrigerant pump for causing the refrigerant to circulate through the circulation channel, and a cooling section arranged on the circulation channel, at a position away from the pump head, the cooling section being configured to cool the refrigerant passing through the circulation channel. The liquid carbon dioxide delivery pump further has a temperature sensor for detecting an ambient temperature of the pump head or a temperature of the pump head, and a refrigerant pump control section for adjusting, based on a detection signal of the temperature sensor, a flow rate of the refrigerant pump such that liquid carbon dioxide flowing through the pump head at a specific temperature.

A Chromatography system that includes a microfluidic device configured to provide three-way switching or switching between three or more inputs or outputs. The microfluidic device fluidically coupled to one or more switching valves to provide for selective control of fluid flow in the chromatography system.

Exemplary embodiments of the present disclosure include systems, apparatuses, and methods that are directed to controlling pressure in a pressurized flow system, such as a CO.sub.2-based chromatography system or other pressurized flow systems. Exemplary embodiments of the present disclosure comprise one or more apparatuses, systems or methods for implementing multiple pressure regulators to control pressure. In addition to providing pressure control, apparatuses, systems and methods described herein dampen damaging thermal effects caused by pressure drops of a mobile phase including CO.sub.2.

Exemplary embodiments of the present disclosure include systems, apparatuses, and methods that are directed to controlling pressure in a pressurized flow system, such as a CO.sub.2-based chromatography system or other pressurized flow systems. Exemplary embodiments of the present disclosure comprise one or more apparatuses, systems or methods for implementing multiple pressure regulators to control pressure. In addition to providing pressure control, apparatuses, systems and methods described herein dampen damaging thermal effects caused by pressure drops of a mobile phase including CO.sub.2.

A sample management device which comprises a source flow path in which a fluidic sample can flow, a volume flow adjustment unit configured for adjusting a volume flow of the fluidic sample to be branched off from the source flow path at a fluidic coupling point, and a fluidic valve fluidically coupled with the source flow path and with the volume flow adjustment unit, wherein the fluidic valve is switchable into a branch off state in which the fluidic coupling point is established within the source flow path to branch off an adjustable volume of the fluidic sample from the source flow path via the fluidic coupling point while a flow of the fluidic sample in the source flow path continues.

A sample management device which comprises a source flow path in which a fluidic sample can flow, a volume flow adjustment unit configured for adjusting a volume flow of the fluidic sample to be branched off from the source flow path at a fluidic coupling point, and a fluidic valve fluidically coupled with the source flow path and with the volume flow adjustment unit, wherein the fluidic valve is switchable into a branch off state in which the fluidic coupling point is established within the source flow path to branch off an adjustable volume of the fluidic sample from the source flow path via the fluidic coupling point while a flow of the fluidic sample in the source flow path continues.