A pressure system for liquid chromatography allows multiple fluid lines to be driven in parallel by a single pump. A fluid pressure medium supply provides fluid pressure to the system. The fluid pressure medium supply is connected to a plurality of fluid transfer pipes, each with a flow limiter. The fluid pressure medium supply is configured to provided more flow than the flow limiters combined, and excess flow is either diverted with a pressure limiter or reduced with a pressure adjuster, or both. Further layers of pressure and flow control may be added with additional pressure limiters and/or pressure adjusters and flow limiters connected together. In some cases, multiple lines containing different fluids may be driven by the same pressure source through the use of flexible fluid delivery inner containers, each within a rigid container that is pressurized by the pressure source.

A pressure system for liquid chromatography allows multiple fluid lines to be driven in parallel by a single pump. A fluid pressure medium supply provides fluid pressure to the system. The fluid pressure medium supply is connected to a plurality of fluid transfer pipes, each with a flow limiter. The fluid pressure medium supply is configured to provided more flow than the flow limiters combined, and excess flow is either diverted with a pressure limiter or reduced with a pressure adjuster, or both. Further layers of pressure and flow control may be added with additional pressure limiters and/or pressure adjusters and flow limiters connected together. In some cases, multiple lines containing different fluids may be driven by the same pressure source through the use of flexible fluid delivery inner containers, each within a rigid container that is pressurized by the pressure source.

Systems for use with liquid chromatography for provision of continuous flow or gradient flow in connection with two pumps providing mobile phase to a valve.

Systems for use with liquid chromatography for provision of continuous flow or gradient flow in connection with two pumps providing mobile phase to a valve.

An autosampler includes a retention path for injecting a sample into a mobile phase that flows through an analysis path to a column, the retention path including a needle at a downstream end and holding the sample, an injection path including a port at an upstream end for injecting into the analysis path, the sample injected from the needle into the port, a low-pressure path having an upstream end connected to a pump, and a valve that can switch between a first state in which an upstream analysis path and a downstream analysis path are connected and a downstream end of the low-pressure path and an upstream end of the retention path are connected and a second state in which the upstream analysis path and the upstream end of the retention path are connected and the downstream end of the injection path and the downstream analysis path are connected.

An autosampler includes a retention path for injecting a sample into a mobile phase that flows through an analysis path to a column, the retention path including a needle at a downstream end and holding the sample, an injection path including a port at an upstream end for injecting into the analysis path, the sample injected from the needle into the port, a low-pressure path having an upstream end connected to a pump, and a valve that can switch between a first state in which an upstream analysis path and a downstream analysis path are connected and a downstream end of the low-pressure path and an upstream end of the retention path are connected and a second state in which the upstream analysis path and the upstream end of the retention path are connected and the downstream end of the injection path and the downstream analysis path are connected.

Described are a method and a fluidic network for acquiring and injecting a chromatographic sample into a chromatography system flow. The fluidic network includes a metering pump module, a sample needle, a needle seal and an injection valve. The metering pump module includes a metering pump and a pressure transducer in serial fluidic communication. When the injection valve is in a first valve state, the injection valve is configured to fluidically terminate ports of the metering pump module. When the injection valve is in a second valve state, the injection valve is configured to fluidically couple a fluidic path that includes the metering pump module and sample needle into the system flow of a chromatography system without resulting in a substantial change in the pressure of the system flow.

Described are a method and a fluidic network for acquiring and injecting a chromatographic sample into a chromatography system flow. The fluidic network includes a metering pump module, a sample needle, a needle seal and an injection valve. The metering pump module includes a metering pump and a pressure transducer in serial fluidic communication. When the injection valve is in a first valve state, the injection valve is configured to fluidically terminate ports of the metering pump module. When the injection valve is in a second valve state, the injection valve is configured to fluidically couple a fluidic path that includes the metering pump module and sample needle into the system flow of a chromatography system without resulting in a substantial change in the pressure of the system flow.

A sample injector for use in a fluid separation system for separating compounds of a fluidic sample in a mobile phase, the sample injector comprising a switchable valve, a sample loop in fluid communication with the valve and configured for receiving the fluidic sample, a metering device in fluid communication with the sample loop and configured for introducing a metered amount of the fluidic sample on the sample loop, and a control unit configured for controlling switching of the valve to transfer the sample loop between a low pressure state and a high pressure state via an intermediate state and for controlling the metering device during the intermediate state to at least partially equilibrate a pressure difference in the sample loop between the low pressure state and the high pressure state.

A sample injector for use in a fluid separation system for separating compounds of a fluidic sample in a mobile phase, the sample injector comprising a switchable valve, a sample loop in fluid communication with the valve and configured for receiving the fluidic sample, a metering device in fluid communication with the sample loop and configured for introducing a metered amount of the fluidic sample on the sample loop, and a control unit configured for controlling switching of the valve to transfer the sample loop between a low pressure state and a high pressure state via an intermediate state and for controlling the metering device during the intermediate state to at least partially equilibrate a pressure difference in the sample loop between the low pressure state and the high pressure state.