The present disclosure is directed to methods for characterization of a sample by size exclusion chromatography (SEC), the sample including intact lipid nanoparticles (LNPs). The method generally includes loading the sample on a chromatographic column having an SEC packing material disposed therein, flowing a mobile phase through the SEC packing material to elute the intact LNPs, and detecting the eluted intact LNPs. The mobile phase includes an organic solvent, an aqueous buffer, and a non-ionic surfactant.

Provided is a method for detecting asiaticoside and madecassoside in cosmetics. Asiaticoside and madecassoside are extracted from cosmetics using a mixed extraction solvent of methanol and acetonitrile, and the asiaticoside and the madecassoside are detected by high performance liquid chromatography (HPLC). By controlling chromatographic conditions and selecting a mobile phase with a specific composition, the asiaticoside and the madecassoside in cosmetics can be assayed.

The present disclosure is directed to liquid chromatography columns and methods utilizing a stationary phase sorbent having two or more gradient factors. Each of the two or more gradient factors varies in a progressive manner along a length of the stationary phase sorbent in a direction from an inlet to an outlet of the column (i.e., along a length of the column). As a result of including these new continuous stationary phase gradients, new selectivities allowing for separation and analysis of complex samples including large biomolecules is achievable.

A combustion gas absorption liquid generation device includes a supplier, a combuster that generates a combustion gas by combusting and decomposing a sample, and an absorber that causes an absorption liquid to absorb the combustion gas generated in the combuster. The supplier supplies the sample supplied from a sample supplier to the combuster, and supplies the absorption liquid supplied from an absorption liquid supplier to the absorber.

The invention discloses a bioprocess chromatography column comprising: a) a bed chamber delimited by at least one side wall, a first bed support screen and a second bed support screen; b) a first end wall, secured to or integral with the side wall(s), with a first port fluidically connected via a first distributor to the first bed support screen; c) a second end wall, secured to or integral with the side wall(s), with a second port fluidically connected via a second distributor to the second bed support screen; d) a packing port in a wall; and e) an internal bracing, secured to, or integral with, at least one of the end walls and extending into the bed chamber.