A new electrospray emitter capable of cone-jet mode spray for liquid flow-rates from sub-microliter/minute to 10's of microliters/minute is provided. The high efficiency of ionization and vaporization enabled by the disclosed spray emitter provides improved sensitivity for mass spectrometer detection. The disclosed spray emitter sprays consistently and robustly a liquid buffer having a large range of compositions of aqueous and organic solvents, and is not sensitive to the polarity of the electric field used to induce spray. The fluidic channel leading to the spray emitter opening does not have an internal taper rendering the spray emitter clog-resistant.

A crescent PLOT column is disclosed, including a capillary column having an inlet, an outlet, a bore, and an inner surface surrounding the bore and extending between the inlet and the outlet. A layer of particles is localized on a radial portion of the inner surface. The layer of the particles includes a radial thickness decreasing from a center of the radial portion to a periphery of the radial portion, forming a crescent shape in a radial frame of reference. A method for preparing the crescent PLOT column is disclosed, including loading the capillary column with a fluid including a carrier and particles such that the fluid is contained within the capillary column. The capillary column and the fluid contained within the capillary column are subjected to a centrifugal force. The carrier is removed, and a layer of the particles is localized on the radial portion of the inner surface.

A measuring arrangement comprising: a support element having a longitudinal axis, wherein a sensor for ascertaining a process variable of a gaseous or liquid fluid is arranged on the support element; and the sensor, wherein the sensor has a fluid duct, which extends within the sensor. The support element has a fluid duct, and for mechanical connection of the fluid duct with the fluid duct of the sensor a bonding layer, which extends over a portion of a surface of the support element and over a portion of a surface of the sensor. The bonding layer comprises at least one fluorinated polymer. The support element has for connection of the fluid duct of the support element with the fluid duct of the sensor, in each case, at least one connection element, which protrude from the support element perpendicularly to the longitudinal axis and which protrude inwardly into the fluid duct of the sensor. The connection elements are secured at least to a the sensor by means of a bonding layer, and wherein the bonding layer comprises a fluorinated polymer.

A large number of kinds of azo compounds which are representative hazardous substances in fiber products are divided into two groups. The compounds included in the first group are detected by an MRM measurement by a tandem mass spectrometer unit (12) in a measurement section (10) while a two-liquid gradient elution under an acidic condition is performed in a liquid chromatograph unit (11), using an aqueous ammonium acetate solution as mobile phase A, and a mixture of acetonitrile and an aqueous ammonium acetate solution as mobile phase B. On the other hand, the compounds included in the second group are detected by an MRM measurement while a two-liquid gradient elution under a neutral or weakly basic condition is performed using an aqueous ammonium bicarbonate solution as mobile phase A and acetonitrile as mobile phase B. An exhaustive quantitative analysis for major azo compounds can be achieved by performing the two analyses for the same sample. An efficient test with a shortened analysis period can thereby be performed.

The present invention relates to a device for preventing band broadening and remixing of separated fractions, and associated method, comprising a chromatographic column coupled to a flow selector, such as a rotary valve, wherein said flow selector is connected to the distal end of said column such that the sum of post-column swept volume and post-column dead volume is less than 10 L. Preferably, the column is directly plugged into the inlet port of the rotary valve and the sample is fractionated at the outlet port.

A fluid transfer device transfers a fluid from a first fluid channel with a first cross-sectional area into a second fluid channel with a second cross-sectional area, larger than the first cross-sectional area. The fluid transfer device includes a fluid inlet interface at which the fluid is transferable from the first fluid channel into the fluid transfer device; an inlet branch configured to split the fluid from the first fluid channel into multiple inlet branch channels; multiple outlet branches, each of which is configured to split the fluid from the inlet branch channels into respective outlet branch channels; and a fluid outlet interface configured to transfer the fluid in the outlet branch channels into the second fluid channel. The inlet and output branches and branch channels are disposed such that the fluid exits from the fluid outlet interface, distributed in a two-dimensional manner across the second cross-sectional area.

A chromatography column assembly includes a standard column length of at least 100 mm and a plurality of columns connected in series. Each of the plurality of columns add up in length to the standard column length. Further disclosed are liquid chromatography systems including the described chromatography assemblies, and chromatographic methods deploying the described chromatographic assemblies.

The present disclosure relates generally to a chromatography column adaptor for forming a fluidic connection between a chromatography column and a GC component. The present disclosure also relates to fluidic connections for a gas chromatography system.

A chromatography column fitting 30 includes a body member 40 configured to be received at an end of a chromatography column 18, the body member 40 defining an axially extending open bore 42. An insert 44 is received in the bore 42, the insert 44 defining a flow conduit 46 opening out into an operatively inner end 50 of the insert 44 to be in flow communication, in use, with an interior 34 of the chromatography column 18, the operatively inner end 50 defining a bearing surface 52. The bearing surface 52 bears against contents 36, 38 at the end of the chromatography column 18 to minimise dead volume between the flow conduit 46 and the contents 36, 38 of the chromatography column 18.

A flat spiral microfabricated gas chromatography column is formed by etching a spiral channel into a planar substrate and bonding additional substrates to each side thereof. Holes or vias in these substrates form an entrance and an exit to the spiral channel etched in the middle substrate. More than one spiral may be etched into a substrate and more than one entrance and exit may be etched into the top and bottom substrates to provide an entrance and an exit to the spiral channel. The spiral channel may have an etched width that is wider than the substrate is thick. In this fashion, a high aspect ratio column is formed with the channel width defining the long dimension of the high aspect ratio column and the thickness of the substrate defining the short dimension of the high aspect ratio column.