A device for separating one or more molecules of interest in a liquid specimen including a monolithic body defining a fractionation column. The column includes an inlet opening at a proximal end of the fractionation column; an outlet opening at a distal, opposite end of the fractionation column; a solid phase chamber positioned between the inlet opening and the outlet opening; a specimen introduction area adjacent a proximal end of the solid phase chamber; an analyte exit area adjacent a distal end of the solid phase chamber; an inlet chamber adjacent the inlet opening that tapers into the specimen introduction area; and an outlet chamber that extends from the analyte exit area to the outlet opening. A metered amount of solid phase packed within the solid phase chamber between a first porous frit and a second porous frit of the solid phase chamber.

Disclosed is a continuous process in which a subset of a number of mutually identical columns, are connected in series. The process liquid, e.g. crude cell culture harvest, is supplied to the most upstream column of the subset. It flows successively through the in series connected columns and leaves the subset through the most downstream and flows into the downstream collection vessel. As soon as the packed bed of the most upstream column is become saturated with product, this column is disconnected from the subset. It is removed from the series connection. A replacement, identical, column is added such that it is connected in series downstream from the most downstream column of the subset. This process is repeated.

Described is a chromatographic column assembly that includes an outer tube comprising a metal, a first conduit disposed within the outer tube, a second conduit disposed within the outer tube, and a first joint located between the first conduit and the second conduit. The outer tube is deformed by a first uniform radial crimp at a longitudinal location along the outer tube that surrounds the first conduit on a first side of the first joint, and a second uniform radial cramp at a longitudinal location along the outer tube that surrounds the second conduit on a second side of the first joint. The first and second uniform radial cramps form a fluid-tight seal between the first conduit and the second conduit and each have a substantially flat base region over which a diameter of the outer tube is reduced for a non-zero longitudinal length.

A chromatography column has a retaining plug permanently fixed to an upstream end of the column and blocks one end of the bore through the column. The plug has a fluid passage therethrough. An upstream end of the passage is preferably but optionally larger in diameter than a downstream end of the passage. An upstream porous member upstream of the retaining plug is held by an upstream end cap and urged toward the plug. Chromatographic media extends from the upstream porous member, through the passage in the retaining plug, to a downstream porous member held by a downstream end cap. The media between the retaining plug and the downstream porous member are under compression to form a bed of packed media.

The present disclosure relates to a filter. The filter includes a porous element, a compression element and a housing. At least a portion of the porous element is coated with an alkylsilyl coating. The compression element is configured to receive the porous element thereby forming an assembly. The housing has an opening formed therein. The opening is configured to receive the assembly. The assembly is retained within the opening when the assembly is received therein.

A static mixer device comprising a housing having a proximal end, a distal end, and an opening extending between the proximal and distal ends. In certain embodiments, a plurality of metal frits is positioned within the opening of the housing, each of the metal frits extending across a cross-sectional dimension of the opening and having interconnected porosity. In other embodiments, one or more mixer elements fabricated using laser additive manufacturing technology and having novel configurations are positioned within the opening of the housing. In yet other embodiments, the housing comprises multiple openings having different diameters from each other, with each opening either extending through the housing with a constant diameter or with one or more of the openings having a varying diameter.

A chromatography column has a column assembly that further includes a column jacket tube and a column liner tube. The column assembly is configured with end fittings that have compression limiting features and are able to withstand very high pressure, such as used in UHPLC, in conjunction with flange portions of the column liner tube that seal the column assembly. The column liner tube may be biocompatible and may have a smooth consistent inner diameter of less than 2 mm, and an inner diameter of 1 mm in particular embodiments. The end fittings for the microtube assembly may ensure durability and reliability by preventing over- or under-tightening.

A slurry chamber is fastened to and held in coaxial alignment with a chromatography column by a V-band. One end of the column has an end cap and frit. A slurry of fluid and chromatographic media is placed in the column and chamber. A piston is forced through the chamber and into the column by a hydraulic ram to expel the fluid and form a media bed in the column. The slurry chamber is removed, and a split end cap is fastened to the column. A threaded recess in the split end cap receives a split, threaded collet. The collet is tightened until it pushes against the piston with the same force as the ram, at which point the ram is released and the column removed for use.

A slurry chamber is fastened to and held in coaxial alignment with a chromatography column by a V-band. One end of the column has an end cap and frit. A slurry of fluid and chromatographic media is placed in the column and chamber. A piston is forced through the chamber and into the column by a hydraulic ram to expel the fluid and form a media bed in the column. The slurry chamber is removed, and a split end cap is fastened to the column. A threaded recess in the split end cap receives a split, threaded collet. The collet is tightened until it pushes against the piston with the same force as the ram, at which point the ram is released and the column removed for use.

A system that incorporates teachings of the subject disclosure may include, for example, a process that includes obtaining a porous medium comprising a porous material having a first shape and an initial porosity profile. The porous medium is engaged with a cavity in a fluidic device, wherein the cavity is in fluid communication with a channel of the fluidic device. The first shape of the porous material can be adjusted to a second shape resulting in the initial porosity profile being adjusted to a target porosity profile. Such adjustment can be accomplished by the engaging of the porous medium with the cavity, by pre-adjusting a shape of the porous media before insertion into the cavity, or by some combination thereof. Other embodiments are disclosed.