Disclosed is a continuous process in which a subset of a number of mutually identical columns, are connected in series. The process liquid, e.g. crude cell culture harvest, is supplied to the most upstream column of the subset. It flows successively through the in series connected columns and leaves the subset through the most downstream and flows into the downstream collection vessel. As soon as the packed bed of the most upstream column is become saturated with product, this column is disconnected from the subset. It is removed from the series connection. A replacement, identical, column is added such that it is connected in series downstream from the most downstream column of the subset. This process is repeated.

Disclosed herein are chromatography columns with conical or reducing inside dimensions and improved fits for use therewith, along with methods for making the columns and the frits.

A chromatography column assembly includes a standard column length of at least 100 mm and a plurality of columns connected in series. Each of the plurality of columns add up in length to the standard column length. Further disclosed are liquid chromatography systems including the described chromatography assemblies, and chromatographic methods deploying the described chromatographic assemblies.

The present disclosure relates to a method of reducing degradation of sample components in a liquid chromatography system. The method utilizes a masked metal frit to prevent or reduce metal surfaces from becoming catalytically active. The masked metal frit is a metal based frit that includes a coating on its exterior surfaces to mask or prevent contact between the organic solvents (and/or any analyte or other related solvent) and the underlying metal. The coating is a vapor deposited inorganic-organic hybrid coating, such as a vapor deposited C2 coating.

The present disclosure relates to a method of reducing degradation of sample components in a liquid chromatography system. The method utilizes a masked metal frit to prevent or reduce metal surfaces from becoming catalytically active. The masked metal frit is a metal based frit that includes a multilayer coating on its exterior surfaces to mask or prevent contact between the organic solvents (and/or any analyte or other related solvent) and the underlying metal. The multilayer coating is a vapor deposited and can include an outer layer formed of an inorganic-organic hybrid material.

A chromatography column fitting 30 includes a body member 40 configured to be received at an end of a chromatography column 18, the body member 40 defining an axially extending open bore 42. An insert 44 is received in the bore 42, the insert 44 defining a flow conduit 46 opening out into an operatively inner end 50 of the insert 44 to be in flow communication, in use, with an interior 34 of the chromatography column 18, the operatively inner end 50 defining a bearing surface 52. The bearing surface 52 bears against contents 36, 38 at the end of the chromatography column 18 to minimise dead volume between the flow conduit 46 and the contents 36, 38 of the chromatography column 18.

A purification receptacle for the chromatographic purification of a liquid. The purification receptacle comprises a seat and a chromatographic media assembly including a chromatographic membrane to which target components contained in a liquid supplied to the receptacle and which passes through the chromatographic membrane bind, and a retaining member configured to compress the chromatographic membrane against the seat. Also disclosed is a chromatographic purification system, a method of separating target components from a liquid sample containing target and non-target components prior to purifying and eluting steps in a chromatographic system, and a chromatographic purification method to isolate a specific target component from a sample liquid.

In some examples, a filter assembly may include a filter including a first gasket having a first channel adjacent to a first side of the filter and a second gasket having a second channel adjacent to a second side of the filter. The first gasket and the second gasket may include a beveled surface adjacent to the filter. The first channel and the second channel may include a diameter of from about 0.01 mm to about 0.5 mm. A finger tightening system may securely hold the filter without any leaks.

In some examples, a filter assembly may include a filter including a first gasket having a first channel adjacent to a first side of the filter and a second gasket having a second channel adjacent to a second side of the filter. The first gasket and the second gasket may include a beveled surface adjacent to the filter. The first channel and the second channel may include a diameter of from about 0.01 mm to about 0.5 mm. A finger tightening system may securely hold the filter without any leaks.