A method of comprising: introducing a sample volume into an inlet end of a liquid chromatography column, wherein the liquid chromatography column includes a focusing segment proximal to the inlet end of the liquid chromatography column and a separation segment proximal to an elute outlet of the liquid chromatography column; maintaining only the focusing segment at a first temperature as the sample is introduced into the focusing segment; and subsequently heating the focusing segment to a second temperature that is higher than the first temperature after the entire sample volume has been introduced into the focusing segment.

The invention relates to tubing assemblies and methods for forming a fluidic connection to make the same. The assemblies include an outer tube having disposed therein a first inner tube affixed to the outer tube, a second inner tube and a support tube disposed in the second inner tube. The second inner tube is abutted against the first inner tube. A radial crimp is made in the outer tube over the second inner tube a distance x from where the tubes abut; the distance x being such that the end of the second inner tube seals against the end of the first inner tube. Various embodiments provide assemblies suitable for use with high fluidic pressure (e.g., greater than an about 70 MPa, approximately about 10,000 psi) and formation of chromatographic column assemblies comprising a capillary tube predisposed within the first or second inner tube prior to formation of the seal.

Disclosed is a continuous process in which a subset of a number of mutually identical columns, are connected in series. The process liquid, e.g. crude cell culture harvest, is supplied to the most upstream column of the subset. It flows successively through the in series connected columns and leaves the subset through the most downstream and flows into the downstream collection vessel. As soon as the packed bed of the most upstream column is become saturated with product, this column is disconnected from the subset. It is removed from the series connection. A replacement, identical, column is added such that it is connected in series downstream from the most downstream column of the subset. This process is repeated.

The invention discloses a method for packing a plurality of uniform chromatography columns, comprising the steps of: a) providing a plurality of chromatography columns; b) providing a plurality of chromatography resin aliquots; c) packing the chromatography resin aliquots in the chromatography columns to provide a plurality of packed chromatography columns; and d) subjecting the packed chromatography columns to repeated mechanical impacts to provide a plurality of uniform chromatography columns.

A clamp assembly includes a rail configured to receive a first fluidic assembly, and a carriage slidably mounted to the rail and configured to receive a second fluidic assembly. The carriage is operable to establish a first fluid tight seal between the first fluidic assembly and a chromatography column received within the clamp assembly, and to establish a second fluid tight seal between the second fluidic assembly and the chromatography column.

Lowering of a switching recovery rate is suppressed without degrading separating ability in a second analytical column. A multidimensional GC includes a sample injector for injecting a sample, a first analytical column for separating the sample injected from the sample injector into one or more components, the first analytical column communicating with the sample injector, a detector for detecting the components of the sample separated in the first analytical column, a second analytical column provided separately from the first analytical column, a switching device connected to an outlet side of the first analytical column and configured to lead the sample out of the first analytical column to the detector or to the second analytical column, and a temperature adjuster configured to adjust temperature of the switching device to a predetermined temperature independently of a temperature of the first analytical column and a temperature of the second analytical column.

A parallel assembly of chromatography column modules, the assembly having one common assembly inlet and one common assembly outlet, each column module comprising a bed space filled with chromatography medium and each column module comprises integrated fluid conduits which when the column module is connected with other column modules are adapted to connect the bed space of the column module with the assembly inlet and the assembly outlet, wherein the total length and/or volume of the fluid conduit from the assembly inlet to one bed space together with the length and/or volume of the fluid conduit from the same bed space to the assembly outlet is substantially the same for all bed spaces and modules installed in the parallel assembly.

A ferrule container 1 is provided with a ferrule accommodating part 11 and a first column guide part 12. A ferrule is accommodated in the ferrule accommodating part 11. The first column guide part 12 guides a column so as to insert the column into the ferrule accommodated in the ferrule accommodating part 11. Guiding of the column by the first column guide part 12 enables the column to be smoothly and reliably inserted into the ferrule accommodated in the ferrule accommodating part 11. Consequently, unlike a case in which an operator inserts a column while pinching a ferrule with hand, the work of inserting a column into a ferrule can easily be performed.

The clamping device according to the invention for clamping at least two processing devices for a sample preparation is made up essentially of at least three clamping blocks arranged in the axial direction, at least two of which are designed as movable clamping blocks, and a displacement mechanism for displacing the movable clamping blocks between an open position for inserting the processing devices, and a clamping position in which the at least two processing devices are clamped one on top of the other in the axial direction between two clamping blocks in each case. The displacement mechanism is designed for displacing the at least two movable clamping blocks in opposite directions.

The invention discloses a flow control block for a stack of chromatography column modules, as well as a stack of chromatography modules comprising at least one flow control block. The flow control block is in a first position or configuration capable of connecting two chromatography column modules, or a chromatography column module and an endpiece, in parallel and in a second position or configuration it is capable of connecting two chromatography column modules, or a chromatography column module and an endpiece, in series.