The present disclosure relates to methodologies, systems, and apparatus for controlling fluid flow within a chromatography system. The chromatography system includes a mobile phase pump configured to pump a liquid mobile phase through a column and a restrictor positioned downstream of the column and upstream of a detector. The system also includes a valve configured to operate in at least two positions. In a first position, the valve is configured to direct the output of the column to bypass the valve and reach the detector, while in the second position the valve directs the output of the column to waste.

The present invention relates to axial flow chromatography columns, methods for separating one or more analytes in a liquid by the use of such columns, and systems employing such columns. The column comprises a first port and a second port the first port and said second port being at essentially the same level or elevation above the level of the bed space on the chromatography column.

Chromatography and synthesis columns, assemblies, components, and methods of assembly and disassembly are disclosed including a support assembly having lifting mechanisms in each of the legs to raise and lower a frame secured to the column, a swing arm and/or carriage guiding movement of a bottom plate of the column, a bolt-free coupling between the main tube and bottom plate of the column, and upper and lower slurry ports in the main tube of the column.

The present disclosure relates to methodologies, systems, and apparatus for controlling fluid flow within a chromatography system. The chromatography system includes a mobile phase pump configured to pump a liquid mobile phase through a column and a restrictor positioned downstream of the column and upstream of a detector. The system also includes a valve configured to operate in at least two positions. In a first position, the valve is configured to direct the output of the column to bypass the valve and reach the detector, while in the second position the valve directs the output of the column to waste.

A method for packing a chromatography column with chromatography media, comprising the steps of: providing a system comprising a column tube (3,19,21) having a closed first end (5) comprising an inlet/outlet (7), a media inlet (15) adjacent a second end of the column tube and an adaptor (9) positioned inside the column tube initially adjacent the second end of the column tube for sliding and sealing contact with an inner face of the column tube, the column tube and adaptor arranged initially such that they define an internal volume and such that the media inlet is in fluid connection with the internal volume; connecting a media slurry source to the media inlet; at least partially filling the internal volume with media slurry via the media inlet; forcing the adaptor towards the first end of the column tube to reduce the internal volume such that the media inlet is no longer in fluid connection with the reduced internal volume.

A method for packing a chromatography column with chromatography media, comprising the steps of: providing a system comprising a column tube (3,19,21) having a closed first end (5) comprising an inlet/outlet (7), a media inlet (15) adjacent a second end of the column tube and an adaptor (9) positioned inside the column tube initially adjacent the second end of the column tube for sliding and sealing contact with an inner face of the column tube, the column tube and adaptor arranged initially such that they define an internal volume and such that the media inlet is in fluid connection with the internal volume; connecting a media slurry source to the media inlet; at least partially filling the internal volume with media slurry via the media inlet; forcing the adaptor towards the first end of the column tube to reduce the internal volume such that the media inlet is no longer in fluid connection with the reduced internal volume.

A method for separating at least one target compound from a feed solution is provided. The method includes filling a bioprocess package with a chromatography resin. The bioprocess package includes a 2D flexible container comprising an interior compartment, a height having an upper half and a lower half, an inlet and an outlet, the inlet and the outlet being disposed on the same half of the 2D flexible container, the channel-forming feature being configured to maintain a fluid flow path that fluidly connects the interior compartment of the flexible container with the outlet. The method further includes flowing a feed solution into the bioprocess package to contact the chromatography resin such that substantially all of the at least one target compound binds to the chromatography resin, washing the chromatography resin in the bioprocess package, and eluting the chromatography resin.

A connection assembly for a chromatograph is used for connection to a flow path of the chromatograph, and includes a tube, a housing and a ferrule. The housing has an opening into which an end portion of the tube forming the flow path is insertable. The ferrule is formed of resin having chemical resistance and holds the tube in the opening of the housing while surrounding the tube. The ferrule has an inner peripheral surface coming into contact with the tube and an outer peripheral surface coming into contact with the housing. The friction coefficient between the inner peripheral surface of the ferrule and the tube, and the friction coefficient between the outer peripheral surface and the housing are equal to or larger than 0.3.

A gas chromatograph with a removable column holder includes a column and a removable column holder. The column is configured for gas chromatography. The removable column holder houses the column. The removable column holder is configured to be removable from the gas chromatograph device with the column. The removable column holder houses only the column. The removable column holder is configured to allow removing and interchanging of the column within the removable column holder. The removable column holder is configured to protect the column within the column holder from mechanical damage and contamination due to handling. The removable column holder is configured to allow a user to install the column into the gas chromatograph without the need for tools. The removable column holder is also configured to reduce the possibility of error when installing the column.

A gas chromatograph with a positioning system for the inlet liner and the column includes a column and the positioning system for the inlet liner and the column. The column is configured for gas chromatography. Wherein, the positioning system for the inlet liner and the column is configured to position the inlet liner and the column with respect to one another. The positioning system for the inlet liner and the column is configured to repeatably and optimally position the inlet liner and the column with respect to one another. The positioning system for the inlet liner and the column positions the inlet liner in a perpendicular orientation to the column.